B cell development in mice with a defective λ5 gene

The surrogate light chain encoded by the two pre‐B cell‐specific genes VpreB and λ5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the λ 5gene results in a depletion of B220+ CD43− IgM− pre‐B cells in bone marrow, and in a delayed appearance b...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:European journal of immunology 1993-06, Vol.23 (6), p.1284-1288
Hauptverfasser: Rolink, Antonius, Karasuyama, Hajime, Grawunder, Ulf, Haasner, Dirk, Kudo, Akira, MeLchers, Fritz
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1288
container_issue 6
container_start_page 1284
container_title European journal of immunology
container_volume 23
creator Rolink, Antonius
Karasuyama, Hajime
Grawunder, Ulf
Haasner, Dirk
Kudo, Akira
MeLchers, Fritz
description The surrogate light chain encoded by the two pre‐B cell‐specific genes VpreB and λ5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the λ 5gene results in a depletion of B220+ CD43− IgM− pre‐B cells in bone marrow, and in a delayed appearance both of CD5+ as well as CD5− surface immunoglobulin (slg)+ B cells in the periphery. In this report we show that DHJH‐rearranged B220− and B220+, CD43+, c‐kit+, sIgM− pro‐ and pre‐B‐I cells with long‐term capacity to proliferate in vitro on stromal cells in the presence of interleukin‐7 are present in normal numbers in the bone marrow of λ 5T/λ.5T mice at various ages. They express normal levels of VpreB mRNA but, in contrast to normal pre‐B‐I cells, do not express surrogate light chain on their surface. Pre‐B‐I cells from fetal liver and bone marrow of λ 5T/λ 5T mice differentiate with normal kinetics and in normal numbers to sIg+, mitogen‐reactive B cells. These results suggest that the delayed generation of sIg+ B cells in the peripheral, mature compartments of CD5+ and CD5− cells could be accounted for by the daily production of approximately 5 × 105 sIg+ B cells from the pre‐B‐I cell pool in the absence of a normal pool of pre‐B‐II cells.
doi_str_mv 10.1002/eji.1830230614
format Article
fullrecord <record><control><sourceid>wiley_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_4812628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EJI1830230614</sourcerecordid><originalsourceid>FETCH-LOGICAL-p1474-85c08f590cc94d87187e025302a1c5b513200d83366d145567c9dfc6648f97e53</originalsourceid><addsrcrecordid>eNpFkE9Lw0AUxBdRsFavnvfgNfW9_Zfdo5ZaKwUveg7r5kW3bGJIQks_m9_Bz2SkYk_DMMMw_Bi7RpghgLilTZyhlSAkGFQnbIJaYKZQ4SmbAKDKhLNwzi76fgMAzmg3YeqeB0qJl7Sl9NnW1Aw8NryOgfguDh_cj1FFYYhb4t9fmr9TQ5fsrPKpp6s_nbLXh8XL_DFbPy9X87t11qLKVWZ1AFtpByE4VdocbU4g9PjQY9BvGqUAKK2UxpSotDZ5cGUVjFG2cjlpOWU3h93W98GnqvNNiH3RdrH23b5QFoURdqy5Q20XE-3_Y4Til0sxcimOXIrF0-ro5A_SS1ZN</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>B cell development in mice with a defective λ5 gene</title><source>Wiley-Blackwell Journals</source><creator>Rolink, Antonius ; Karasuyama, Hajime ; Grawunder, Ulf ; Haasner, Dirk ; Kudo, Akira ; MeLchers, Fritz</creator><creatorcontrib>Rolink, Antonius ; Karasuyama, Hajime ; Grawunder, Ulf ; Haasner, Dirk ; Kudo, Akira ; MeLchers, Fritz</creatorcontrib><description>The surrogate light chain encoded by the two pre‐B cell‐specific genes VpreB and λ5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the λ 5gene results in a depletion of B220+ CD43− IgM− pre‐B cells in bone marrow, and in a delayed appearance both of CD5+ as well as CD5− surface immunoglobulin (slg)+ B cells in the periphery. In this report we show that DHJH‐rearranged B220− and B220+, CD43+, c‐kit+, sIgM− pro‐ and pre‐B‐I cells with long‐term capacity to proliferate in vitro on stromal cells in the presence of interleukin‐7 are present in normal numbers in the bone marrow of λ 5T/λ.5T mice at various ages. They express normal levels of VpreB mRNA but, in contrast to normal pre‐B‐I cells, do not express surrogate light chain on their surface. Pre‐B‐I cells from fetal liver and bone marrow of λ 5T/λ 5T mice differentiate with normal kinetics and in normal numbers to sIg+, mitogen‐reactive B cells. These results suggest that the delayed generation of sIg+ B cells in the peripheral, mature compartments of CD5+ and CD5− cells could be accounted for by the daily production of approximately 5 × 105 sIg+ B cells from the pre‐B‐I cell pool in the absence of a normal pool of pre‐B‐II cells.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.1830230614</identifier><identifier>CODEN: EJIMAF</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>B cell development ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; Knock‐out mice ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; δs gene</subject><ispartof>European journal of immunology, 1993-06, Vol.23 (6), p.1284-1288</ispartof><rights>Copyright © 1993 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Feji.1830230614$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Feji.1830230614$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=4812628$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Rolink, Antonius</creatorcontrib><creatorcontrib>Karasuyama, Hajime</creatorcontrib><creatorcontrib>Grawunder, Ulf</creatorcontrib><creatorcontrib>Haasner, Dirk</creatorcontrib><creatorcontrib>Kudo, Akira</creatorcontrib><creatorcontrib>MeLchers, Fritz</creatorcontrib><title>B cell development in mice with a defective λ5 gene</title><title>European journal of immunology</title><description>The surrogate light chain encoded by the two pre‐B cell‐specific genes VpreB and λ5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the λ 5gene results in a depletion of B220+ CD43− IgM− pre‐B cells in bone marrow, and in a delayed appearance both of CD5+ as well as CD5− surface immunoglobulin (slg)+ B cells in the periphery. In this report we show that DHJH‐rearranged B220− and B220+, CD43+, c‐kit+, sIgM− pro‐ and pre‐B‐I cells with long‐term capacity to proliferate in vitro on stromal cells in the presence of interleukin‐7 are present in normal numbers in the bone marrow of λ 5T/λ.5T mice at various ages. They express normal levels of VpreB mRNA but, in contrast to normal pre‐B‐I cells, do not express surrogate light chain on their surface. Pre‐B‐I cells from fetal liver and bone marrow of λ 5T/λ 5T mice differentiate with normal kinetics and in normal numbers to sIg+, mitogen‐reactive B cells. These results suggest that the delayed generation of sIg+ B cells in the peripheral, mature compartments of CD5+ and CD5− cells could be accounted for by the daily production of approximately 5 × 105 sIg+ B cells from the pre‐B‐I cell pool in the absence of a normal pool of pre‐B‐II cells.</description><subject>B cell development</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Knock‐out mice</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>δs gene</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNpFkE9Lw0AUxBdRsFavnvfgNfW9_Zfdo5ZaKwUveg7r5kW3bGJIQks_m9_Bz2SkYk_DMMMw_Bi7RpghgLilTZyhlSAkGFQnbIJaYKZQ4SmbAKDKhLNwzi76fgMAzmg3YeqeB0qJl7Sl9NnW1Aw8NryOgfguDh_cj1FFYYhb4t9fmr9TQ5fsrPKpp6s_nbLXh8XL_DFbPy9X87t11qLKVWZ1AFtpByE4VdocbU4g9PjQY9BvGqUAKK2UxpSotDZ5cGUVjFG2cjlpOWU3h93W98GnqvNNiH3RdrH23b5QFoURdqy5Q20XE-3_Y4Til0sxcimOXIrF0-ro5A_SS1ZN</recordid><startdate>199306</startdate><enddate>199306</enddate><creator>Rolink, Antonius</creator><creator>Karasuyama, Hajime</creator><creator>Grawunder, Ulf</creator><creator>Haasner, Dirk</creator><creator>Kudo, Akira</creator><creator>MeLchers, Fritz</creator><general>WILEY‐VCH Verlag GmbH</general><general>Wiley-VCH</general><scope>IQODW</scope></search><sort><creationdate>199306</creationdate><title>B cell development in mice with a defective λ5 gene</title><author>Rolink, Antonius ; Karasuyama, Hajime ; Grawunder, Ulf ; Haasner, Dirk ; Kudo, Akira ; MeLchers, Fritz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1474-85c08f590cc94d87187e025302a1c5b513200d83366d145567c9dfc6648f97e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>B cell development</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Knock‐out mice</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>δs gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rolink, Antonius</creatorcontrib><creatorcontrib>Karasuyama, Hajime</creatorcontrib><creatorcontrib>Grawunder, Ulf</creatorcontrib><creatorcontrib>Haasner, Dirk</creatorcontrib><creatorcontrib>Kudo, Akira</creatorcontrib><creatorcontrib>MeLchers, Fritz</creatorcontrib><collection>Pascal-Francis</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rolink, Antonius</au><au>Karasuyama, Hajime</au><au>Grawunder, Ulf</au><au>Haasner, Dirk</au><au>Kudo, Akira</au><au>MeLchers, Fritz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>B cell development in mice with a defective λ5 gene</atitle><jtitle>European journal of immunology</jtitle><date>1993-06</date><risdate>1993</risdate><volume>23</volume><issue>6</issue><spage>1284</spage><epage>1288</epage><pages>1284-1288</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><coden>EJIMAF</coden><abstract>The surrogate light chain encoded by the two pre‐B cell‐specific genes VpreB and λ5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the λ 5gene results in a depletion of B220+ CD43− IgM− pre‐B cells in bone marrow, and in a delayed appearance both of CD5+ as well as CD5− surface immunoglobulin (slg)+ B cells in the periphery. In this report we show that DHJH‐rearranged B220− and B220+, CD43+, c‐kit+, sIgM− pro‐ and pre‐B‐I cells with long‐term capacity to proliferate in vitro on stromal cells in the presence of interleukin‐7 are present in normal numbers in the bone marrow of λ 5T/λ.5T mice at various ages. They express normal levels of VpreB mRNA but, in contrast to normal pre‐B‐I cells, do not express surrogate light chain on their surface. Pre‐B‐I cells from fetal liver and bone marrow of λ 5T/λ 5T mice differentiate with normal kinetics and in normal numbers to sIg+, mitogen‐reactive B cells. These results suggest that the delayed generation of sIg+ B cells in the peripheral, mature compartments of CD5+ and CD5− cells could be accounted for by the daily production of approximately 5 × 105 sIg+ B cells from the pre‐B‐I cell pool in the absence of a normal pool of pre‐B‐II cells.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><doi>10.1002/eji.1830230614</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0014-2980
ispartof European journal of immunology, 1993-06, Vol.23 (6), p.1284-1288
issn 0014-2980
1521-4141
language eng
recordid cdi_pascalfrancis_primary_4812628
source Wiley-Blackwell Journals
subjects B cell development
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Knock‐out mice
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
δs gene
title B cell development in mice with a defective λ5 gene
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A36%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=B%20cell%20development%20in%20mice%20with%20a%20defective%20%CE%BB5%20gene&rft.jtitle=European%20journal%20of%20immunology&rft.au=Rolink,%20Antonius&rft.date=1993-06&rft.volume=23&rft.issue=6&rft.spage=1284&rft.epage=1288&rft.pages=1284-1288&rft.issn=0014-2980&rft.eissn=1521-4141&rft.coden=EJIMAF&rft_id=info:doi/10.1002/eji.1830230614&rft_dat=%3Cwiley_pasca%3EEJI1830230614%3C/wiley_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true