Toxic Effects and Detection of Oxygen Free Radicals on Cultured Articular Chondrocytes Generated by Menadione

The aim of this work was to study the proliferation pathological perturbations of cultured chondrocytes in response to menadione, an oxygen free radicals producing drug. Rabbit articular chondrocytes in monolayer culture were treated with 10−5, 1.5.M−5 and 2.10−5M of menadione during three days. A d...

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Veröffentlicht in:Free radical research 1992, Vol.17 (4), p.279-289
Hauptverfasser: Michel, Christine, Vincent, Francoise, Duval, Christine, Poelman, Marie-Christine, Adolphe, Monique
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container_end_page 289
container_issue 4
container_start_page 279
container_title Free radical research
container_volume 17
creator Michel, Christine
Vincent, Francoise
Duval, Christine
Poelman, Marie-Christine
Adolphe, Monique
description The aim of this work was to study the proliferation pathological perturbations of cultured chondrocytes in response to menadione, an oxygen free radicals producing drug. Rabbit articular chondrocytes in monolayer culture were treated with 10−5, 1.5.M−5 and 2.10−5M of menadione during three days. A dose dependent decrease of the proliferative capacity was observed. Flow cytometry analysis revealed a perturbation of the cell cycle progression consisting in an accumulation of cells in the S and G2 + M phases. This growth perturbation was due to oxygen radicals production since a treatment with catalase suppressed these toxic effects. Furthermore, to identify oxygen derived radicals in the cellular suspension of cultures treated with menadione, we used a technique of spin-trapping coupled with electron spin resonance (ESR). The ESR signal corresponding to the DMPO hydroxyl radical adduct (DMPO-OH) has been detected. The spectra observation indicated the actual production of hydroxyl radical. However, superoxide anions have not been identified; this fact can be explained by the low reactivity of these anions with DMPO and by the decomposition of signal DMPO-OOH to DMPO-OH.
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Rabbit articular chondrocytes in monolayer culture were treated with 10−5, 1.5.M−5 and 2.10−5M of menadione during three days. A dose dependent decrease of the proliferative capacity was observed. Flow cytometry analysis revealed a perturbation of the cell cycle progression consisting in an accumulation of cells in the S and G2 + M phases. This growth perturbation was due to oxygen radicals production since a treatment with catalase suppressed these toxic effects. Furthermore, to identify oxygen derived radicals in the cellular suspension of cultures treated with menadione, we used a technique of spin-trapping coupled with electron spin resonance (ESR). The ESR signal corresponding to the DMPO hydroxyl radical adduct (DMPO-OH) has been detected. The spectra observation indicated the actual production of hydroxyl radical. 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Rabbit articular chondrocytes in monolayer culture were treated with 10−5, 1.5.M−5 and 2.10−5M of menadione during three days. A dose dependent decrease of the proliferative capacity was observed. Flow cytometry analysis revealed a perturbation of the cell cycle progression consisting in an accumulation of cells in the S and G2 + M phases. This growth perturbation was due to oxygen radicals production since a treatment with catalase suppressed these toxic effects. Furthermore, to identify oxygen derived radicals in the cellular suspension of cultures treated with menadione, we used a technique of spin-trapping coupled with electron spin resonance (ESR). The ESR signal corresponding to the DMPO hydroxyl radical adduct (DMPO-OH) has been detected. The spectra observation indicated the actual production of hydroxyl radical. However, superoxide anions have not been identified; this fact can be explained by the low reactivity of these anions with DMPO and by the decomposition of signal DMPO-OOH to DMPO-OH.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - drug effects</subject><subject>catalase</subject><subject>Catalase - pharmacology</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>cultured chondrocytes</subject><subject>Effects of physical and chemical agents</subject><subject>electron spin resonance</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Flow Cytometry</subject><subject>free radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Menadione</subject><subject>Molecular and cellular biology</subject><subject>Rabbits</subject><subject>Reactive Oxygen Species - analysis</subject><subject>Vitamin E - pharmacology</subject><subject>Vitamin K - toxicity</subject><issn>1071-5762</issn><issn>8755-0199</issn><issn>1029-2470</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMo3h_AhZCF22ouTTJFNzJeYWRAdF3S9MSpdBJJUrRvb2S8IIKrHPi_75DzI3RAyTGnpDqhRFGhZMVIRVQlGFlD25SwqmClIusfs6JFBtgW2onxmRDKS6E20SblXJScbKPlg3_rDL60FkyKWLsWX0DKc-cd9hbP38YncPgqAOB73XZG9xHnaDr0aQjQ4vOQOjP0OuDpwrs2eDMmiPgaHASdMtCM-A5cVr2DPbRh8wLY_3x30ePV5cP0ppjNr2-n57PCCCZSIbUSZatkIw0wwWBSVcBLLiulFZUUgDVKlyWhmpNJKxWxlMlGcCKssBMq-C6iq70m-BgD2PoldEsdxpqS-qO5-k9z2TlcOS9Ds4T2x1hVlfOjz1zH3IIN2pkufmOl5FyqScbOVljnrA9L_epD39ZJj70PXw7_7xenv_QF6D4tjA5QP_shuNzaPze8A0IvmlQ</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Michel, Christine</creator><creator>Vincent, Francoise</creator><creator>Duval, Christine</creator><creator>Poelman, Marie-Christine</creator><creator>Adolphe, Monique</creator><general>Informa UK Ltd</general><general>Taylor &amp; Francis</general><general>Harwood</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1992</creationdate><title>Toxic Effects and Detection of Oxygen Free Radicals on Cultured Articular Chondrocytes Generated by Menadione</title><author>Michel, Christine ; Vincent, Francoise ; Duval, Christine ; Poelman, Marie-Christine ; Adolphe, Monique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-6a754d76b6ce252e899e343697a7161ee2b7a4401a308d670f126b5305f5f8153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cartilage, Articular - cytology</topic><topic>Cartilage, Articular - drug effects</topic><topic>catalase</topic><topic>Catalase - pharmacology</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>cultured chondrocytes</topic><topic>Effects of physical and chemical agents</topic><topic>electron spin resonance</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Flow Cytometry</topic><topic>free radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Menadione</topic><topic>Molecular and cellular biology</topic><topic>Rabbits</topic><topic>Reactive Oxygen Species - analysis</topic><topic>Vitamin E - pharmacology</topic><topic>Vitamin K - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michel, Christine</creatorcontrib><creatorcontrib>Vincent, Francoise</creatorcontrib><creatorcontrib>Duval, Christine</creatorcontrib><creatorcontrib>Poelman, Marie-Christine</creatorcontrib><creatorcontrib>Adolphe, Monique</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michel, Christine</au><au>Vincent, Francoise</au><au>Duval, Christine</au><au>Poelman, Marie-Christine</au><au>Adolphe, Monique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxic Effects and Detection of Oxygen Free Radicals on Cultured Articular Chondrocytes Generated by Menadione</atitle><jtitle>Free radical research</jtitle><addtitle>Free Radic Res Commun</addtitle><date>1992</date><risdate>1992</risdate><volume>17</volume><issue>4</issue><spage>279</spage><epage>289</epage><pages>279-289</pages><issn>1071-5762</issn><issn>8755-0199</issn><eissn>1029-2470</eissn><abstract>The aim of this work was to study the proliferation pathological perturbations of cultured chondrocytes in response to menadione, an oxygen free radicals producing drug. Rabbit articular chondrocytes in monolayer culture were treated with 10−5, 1.5.M−5 and 2.10−5M of menadione during three days. A dose dependent decrease of the proliferative capacity was observed. Flow cytometry analysis revealed a perturbation of the cell cycle progression consisting in an accumulation of cells in the S and G2 + M phases. This growth perturbation was due to oxygen radicals production since a treatment with catalase suppressed these toxic effects. Furthermore, to identify oxygen derived radicals in the cellular suspension of cultures treated with menadione, we used a technique of spin-trapping coupled with electron spin resonance (ESR). The ESR signal corresponding to the DMPO hydroxyl radical adduct (DMPO-OH) has been detected. The spectra observation indicated the actual production of hydroxyl radical. However, superoxide anions have not been identified; this fact can be explained by the low reactivity of these anions with DMPO and by the decomposition of signal DMPO-OOH to DMPO-OH.</abstract><cop>Chur</cop><cop>Reading</cop><cop>Paris</cop><pub>Informa UK Ltd</pub><pmid>1335430</pmid><doi>10.3109/10715769209079520</doi><tpages>11</tpages></addata></record>
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source MEDLINE; Taylor & Francis:Master (3349 titles)
subjects Animals
Biological and medical sciences
Cartilage, Articular - cytology
Cartilage, Articular - drug effects
catalase
Catalase - pharmacology
Cell Cycle - drug effects
Cell Division - drug effects
Cell physiology
Cells, Cultured
cultured chondrocytes
Effects of physical and chemical agents
electron spin resonance
Electron Spin Resonance Spectroscopy
Flow Cytometry
free radicals
Fundamental and applied biological sciences. Psychology
Menadione
Molecular and cellular biology
Rabbits
Reactive Oxygen Species - analysis
Vitamin E - pharmacology
Vitamin K - toxicity
title Toxic Effects and Detection of Oxygen Free Radicals on Cultured Articular Chondrocytes Generated by Menadione
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