Detection of Herpes Simplex and Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis from Clinical Materials
A simple method using field-inversion gel electrophoresis (FIGE) was applied to detect herpes simplex virus (HSV) and varicella-zoster virus (VZV) genomes in clinical specimens. The whole genomes of these viruses could be detected in small vesicle tissues by the FIGE method regardless of their clini...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1992, Vol.36(11), pp.1217-1221 |
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| container_issue | 11 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
| container_volume | 36 |
| creator | Arao, Yujiro Yoshida, Mariko Sata, Tetsutaro Nakatsukasa, Akihiko Miyoshi, Kaoru Yamada, Masao Uno, Fumio Kurata, Takeshi Nii, Shiro |
| description | A simple method using field-inversion gel electrophoresis (FIGE) was applied to detect herpes simplex virus (HSV) and varicella-zoster virus (VZV) genomes in clinical specimens. The whole genomes of these viruses could be detected in small vesicle tissues by the FIGE method regardless of their clinical stages of skin lesions. And the sensitivity of the FIGE method was equivalent to that of an immunofluorescent (IF) method. These data indicated usefulness of the FIGE method to detect the whole genomes of HSV and VZV in clinical specimens. |
| doi_str_mv | 10.1111/j.1348-0421.1992.tb02125.x |
| format | Article |
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The whole genomes of these viruses could be detected in small vesicle tissues by the FIGE method regardless of their clinical stages of skin lesions. And the sensitivity of the FIGE method was equivalent to that of an immunofluorescent (IF) method. These data indicated usefulness of the FIGE method to detect the whole genomes of HSV and VZV in clinical specimens.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1992.tb02125.x</identifier><identifier>PMID: 1337135</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>HOBOKEN: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; DNA, Viral - analysis ; Electrophoresis, Agar Gel - methods ; Fundamental and applied biological sciences. Psychology ; Herpes Simplex - diagnosis ; Herpes simplex virus ; Herpes Zoster - diagnosis ; Herpesvirus 3, Human - genetics ; Herpesvirus 3, Human - isolation & purification ; Humans ; Immunology ; Life Sciences & Biomedicine ; Microbiology ; Science & Technology ; Sensitivity and Specificity ; Simplexvirus - genetics ; Simplexvirus - isolation & purification ; Techniques used in virology ; Varicella-zoster virus ; Virology</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1992, Vol.36(11), pp.1217-1221</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992KF19800011</woscitedreferencesoriginalsourcerecordid><cites>FETCH-LOGICAL-c6355-d86e6d1ac933806718ee5ae5767dc049382d77a94a1ac9e64f9b25e1f0d782e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27197,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4555960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1337135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arao, Yujiro</creatorcontrib><creatorcontrib>Yoshida, Mariko</creatorcontrib><creatorcontrib>Sata, Tetsutaro</creatorcontrib><creatorcontrib>Nakatsukasa, Akihiko</creatorcontrib><creatorcontrib>Miyoshi, Kaoru</creatorcontrib><creatorcontrib>Yamada, Masao</creatorcontrib><creatorcontrib>Uno, Fumio</creatorcontrib><creatorcontrib>Kurata, Takeshi</creatorcontrib><creatorcontrib>Nii, Shiro</creatorcontrib><title>Detection of Herpes Simplex and Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis from Clinical Materials</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>MICROBIOL IMMUNOL</addtitle><addtitle>Microbiology and Immunology</addtitle><description>A simple method using field-inversion gel electrophoresis (FIGE) was applied to detect herpes simplex virus (HSV) and varicella-zoster virus (VZV) genomes in clinical specimens. The whole genomes of these viruses could be detected in small vesicle tissues by the FIGE method regardless of their clinical stages of skin lesions. And the sensitivity of the FIGE method was equivalent to that of an immunofluorescent (IF) method. These data indicated usefulness of the FIGE method to detect the whole genomes of HSV and VZV in clinical specimens.</description><subject>Biological and medical sciences</subject><subject>DNA, Viral - analysis</subject><subject>Electrophoresis, Agar Gel - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpes Simplex - diagnosis</subject><subject>Herpes simplex virus</subject><subject>Herpes Zoster - diagnosis</subject><subject>Herpesvirus 3, Human - genetics</subject><subject>Herpesvirus 3, Human - isolation & purification</subject><subject>Humans</subject><subject>Immunology</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiology</subject><subject>Science & Technology</subject><subject>Sensitivity and Specificity</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - isolation & purification</subject><subject>Techniques used in virology</subject><subject>Varicella-zoster virus</subject><subject>Virology</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqVkluP0zAQRiMEWpaFn4BkIYSQUIodx5fwRJXdXsS2gIBF4sVynQnrkibFTqH99zibqMsTlzzEkebMGTnfRNETgkckPC_XI0JTGeM0ISOSZcmoXeGEJGy0vxOdHkt3o1NMJYsZx_h-9MD7NcaJSGR6Ep0QSgWh7DQ6nEMLprVNjZoSzcBtwaMPdrOtYI90XaAr7ayBqtLxl8a34NCVdTuPzpdjtDqgiYWqiOf1D3C-c0yhQhdVELpme9048Naj0jUblFe2tkZXaKGDxOrKP4zuleGAR8N5Fn2aXHzMZ_Hl2-k8H1_GhlPG4kJy4AXRJqNUYi6IBGAamOCiMDjNqEwKIXSW6o4BnpbZKmFASlwImUBKz6JnvXfrmu878K3aWH9zoxqanVeCpikTVPwVJDylBIsOfP5nMM0STgiW3fBXPWpc472DUm2d3Wh3UASrLku1Vl1gqgtMdVmqIUu1D82Phzm71QaK29Y-vFB_OtS1D7-2dLo21h-xlDGWcRywFz32E1ZN6Y2F2sCRGndD30xIJjHGhARa_jud21Z3u5M3u7oNra-HVlvB4T9uqhbzxc1nUMx6xdq3-uvtXO1aaypQm7CSlmRCKMqDdXiThIgjYq61U1AHVdyrbFjb_W-mb4qHuJn6vJwq-X6Kl4tcqnf0FxM_BNM</recordid><startdate>19920101</startdate><enddate>19920101</enddate><creator>Arao, Yujiro</creator><creator>Yoshida, Mariko</creator><creator>Sata, Tetsutaro</creator><creator>Nakatsukasa, Akihiko</creator><creator>Miyoshi, Kaoru</creator><creator>Yamada, Masao</creator><creator>Uno, Fumio</creator><creator>Kurata, Takeshi</creator><creator>Nii, Shiro</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Wiley</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920101</creationdate><title>Detection of Herpes Simplex and Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis from Clinical Materials</title><author>Arao, Yujiro ; Yoshida, Mariko ; Sata, Tetsutaro ; Nakatsukasa, Akihiko ; Miyoshi, Kaoru ; Yamada, Masao ; Uno, Fumio ; Kurata, Takeshi ; Nii, Shiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6355-d86e6d1ac933806718ee5ae5767dc049382d77a94a1ac9e64f9b25e1f0d782e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>DNA, Viral - analysis</topic><topic>Electrophoresis, Agar Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpes Simplex - diagnosis</topic><topic>Herpes simplex virus</topic><topic>Herpes Zoster - diagnosis</topic><topic>Herpesvirus 3, Human - genetics</topic><topic>Herpesvirus 3, Human - isolation & purification</topic><topic>Humans</topic><topic>Immunology</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiology</topic><topic>Science & Technology</topic><topic>Sensitivity and Specificity</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - isolation & purification</topic><topic>Techniques used in virology</topic><topic>Varicella-zoster virus</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arao, Yujiro</creatorcontrib><creatorcontrib>Yoshida, Mariko</creatorcontrib><creatorcontrib>Sata, Tetsutaro</creatorcontrib><creatorcontrib>Nakatsukasa, Akihiko</creatorcontrib><creatorcontrib>Miyoshi, Kaoru</creatorcontrib><creatorcontrib>Yamada, Masao</creatorcontrib><creatorcontrib>Uno, Fumio</creatorcontrib><creatorcontrib>Kurata, Takeshi</creatorcontrib><creatorcontrib>Nii, Shiro</creatorcontrib><collection>Istex</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arao, Yujiro</au><au>Yoshida, Mariko</au><au>Sata, Tetsutaro</au><au>Nakatsukasa, Akihiko</au><au>Miyoshi, Kaoru</au><au>Yamada, Masao</au><au>Uno, Fumio</au><au>Kurata, Takeshi</au><au>Nii, Shiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Herpes Simplex and Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis from Clinical Materials</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><stitle>MICROBIOL IMMUNOL</stitle><addtitle>Microbiology and Immunology</addtitle><date>1992-01-01</date><risdate>1992</risdate><volume>36</volume><issue>11</issue><spage>1217</spage><epage>1221</epage><pages>1217-1221</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>A simple method using field-inversion gel electrophoresis (FIGE) was applied to detect herpes simplex virus (HSV) and varicella-zoster virus (VZV) genomes in clinical specimens. 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| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1992, Vol.36(11), pp.1217-1221 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_4555960 |
| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; Alma/SFX Local Collection |
| subjects | Biological and medical sciences DNA, Viral - analysis Electrophoresis, Agar Gel - methods Fundamental and applied biological sciences. Psychology Herpes Simplex - diagnosis Herpes simplex virus Herpes Zoster - diagnosis Herpesvirus 3, Human - genetics Herpesvirus 3, Human - isolation & purification Humans Immunology Life Sciences & Biomedicine Microbiology Science & Technology Sensitivity and Specificity Simplexvirus - genetics Simplexvirus - isolation & purification Techniques used in virology Varicella-zoster virus Virology |
| title | Detection of Herpes Simplex and Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis from Clinical Materials |
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