Erythropoietin Receptor mRNA Expression in Human Endothelial Cells
A previous report demonstrated that endothelial cells have erythropoietin receptors and respond to this hormone with enhanced proliferation. The present study demonstrates the existence of mRNA for erythropoietin receptor in human umbilical vein endothelial cells. We have reverse transcribed mRNA of...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-04, Vol.91 (9), p.3974-3978 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Anagnostou, Athanasius Liu, Ziyao Steiner, Manfred Chin, Kyung Lee, Eun S. Kessimian, Noubar Noguchi, Constance T. |
| description | A previous report demonstrated that endothelial cells have erythropoietin receptors and respond to this hormone with enhanced proliferation. The present study demonstrates the existence of mRNA for erythropoietin receptor in human umbilical vein endothelial cells. We have reverse transcribed mRNA of endothelial cells and then used different PCR primers to amplify erythropoietin receptor target cDNA between exons 5 and 6 as well as 3-5 in addition to an internal standard DNA fragment. Correspondence of size as well as location of restriction endonuclease scission (Ava II) was used in comparing the amplified fragments of human endothelial cell erythropoietin receptor to those of two human erythroleukemia cell lines, OCIM1 and K562. No α- or γ-globin mRNA was detected in endothelial cells but was readily demonstrable in OCIM1 cells. In addition, to determine whether the expression of human erythropoietin receptor on endothelial cells occurs in vivo, sections of umbilical cord and placenta were immunostained with antibodies against the extracellular portion of the receptor; the results showed strong positive staining of the vascular endothelium |
| doi_str_mv | 10.1073/pnas.91.9.3974 |
| format | Article |
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The present study demonstrates the existence of mRNA for erythropoietin receptor in human umbilical vein endothelial cells. We have reverse transcribed mRNA of endothelial cells and then used different PCR primers to amplify erythropoietin receptor target cDNA between exons 5 and 6 as well as 3-5 in addition to an internal standard DNA fragment. Correspondence of size as well as location of restriction endonuclease scission (Ava II) was used in comparing the amplified fragments of human endothelial cell erythropoietin receptor to those of two human erythroleukemia cell lines, OCIM1 and K562. No α- or γ-globin mRNA was detected in endothelial cells but was readily demonstrable in OCIM1 cells. In addition, to determine whether the expression of human erythropoietin receptor on endothelial cells occurs in vivo, sections of umbilical cord and placenta were immunostained with antibodies against the extracellular portion of the receptor; the results showed strong positive staining of the vascular endothelium</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.9.3974</identifier><identifier>PMID: 8171022</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Base Sequence ; Biochemistry ; Biological and medical sciences ; Cell lines ; Cell receptors ; Cell structures and functions ; Complementary DNA ; DNA Primers - chemistry ; Endothelial cells ; Endothelium, Vascular - physiology ; Erythroid cells ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; HeLa cells ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; Human umbilical vein endothelial cells ; Humans ; Immunoenzyme Techniques ; In Vitro Techniques ; K562 cells ; Medical research ; Messenger RNA ; Molecular and cellular biology ; Molecular Sequence Data ; Polymerase chain reaction ; Receptors, Erythropoietin - genetics ; Receptors, Erythropoietin - metabolism ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; Umbilical cord</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-04, Vol.91 (9), p.3974-3978</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Apr 26, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5254-2fc464251132d8d537856ecde1672c38d2dc3ae31e4a1ba4ac90527b592d447a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2364574$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2364574$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4246684$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8171022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anagnostou, Athanasius</creatorcontrib><creatorcontrib>Liu, Ziyao</creatorcontrib><creatorcontrib>Steiner, Manfred</creatorcontrib><creatorcontrib>Chin, Kyung</creatorcontrib><creatorcontrib>Lee, Eun S.</creatorcontrib><creatorcontrib>Kessimian, Noubar</creatorcontrib><creatorcontrib>Noguchi, Constance T.</creatorcontrib><title>Erythropoietin Receptor mRNA Expression in Human Endothelial Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A previous report demonstrated that endothelial cells have erythropoietin receptors and respond to this hormone with enhanced proliferation. The present study demonstrates the existence of mRNA for erythropoietin receptor in human umbilical vein endothelial cells. We have reverse transcribed mRNA of endothelial cells and then used different PCR primers to amplify erythropoietin receptor target cDNA between exons 5 and 6 as well as 3-5 in addition to an internal standard DNA fragment. Correspondence of size as well as location of restriction endonuclease scission (Ava II) was used in comparing the amplified fragments of human endothelial cell erythropoietin receptor to those of two human erythroleukemia cell lines, OCIM1 and K562. No α- or γ-globin mRNA was detected in endothelial cells but was readily demonstrable in OCIM1 cells. In addition, to determine whether the expression of human erythropoietin receptor on endothelial cells occurs in vivo, sections of umbilical cord and placenta were immunostained with antibodies against the extracellular portion of the receptor; the results showed strong positive staining of the vascular endothelium</description><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell lines</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Complementary DNA</subject><subject>DNA Primers - chemistry</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - physiology</subject><subject>Erythroid cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>HeLa cells</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>Human umbilical vein endothelial cells</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>In Vitro Techniques</subject><subject>K562 cells</subject><subject>Medical research</subject><subject>Messenger RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Polymerase chain reaction</subject><subject>Receptors, Erythropoietin - genetics</subject><subject>Receptors, Erythropoietin - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>Umbilical cord</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9rFDEcxYModVu9elIYpPQ2Y35nAr3UZbVCUSh6DtlMxs2SScZkRtr_3iy7LqsHPeXwPt-XvO8LAK8QbBAU5N0YdG4kamRDpKBPwAJBiWpOJXwKFhBiUbcU0-fgPOcthFCyFp6BsxYJBDFegPer9DhtUhyjs5ML1b01dpxiqob7zzfV6mFMNmcXQ1W023nQoVqFLk4b65321dJ6n1-AZ7322b48nBfg24fV1-Vtfffl46flzV1tGGa0xr2hnGKGEMFd2zEiWsat6SziAhvSdrgzRFuCLNVorak2EjIs1kzijlKhyQW43vuO83qwnbFhStqrMblBp0cVtVN_KsFt1Pf4U1EiICvjV4fxFH_MNk9qcNmUADrYOGclOOWMQvlfEPGWwbK-Ar79C9zGOYWyA4UhIiUeaQvU7CGTYs7J9scHI6h2Dapdg0oiJdWuwTLw5jTmET9UVvTLg66z0b5POhiXj1gpm_P21GZn_1s9vebqX7rqZ-8n-zAV8PUe3ObyL44kJpyy4vMLAbXEVw</recordid><startdate>19940426</startdate><enddate>19940426</enddate><creator>Anagnostou, Athanasius</creator><creator>Liu, Ziyao</creator><creator>Steiner, Manfred</creator><creator>Chin, Kyung</creator><creator>Lee, Eun S.</creator><creator>Kessimian, Noubar</creator><creator>Noguchi, Constance T.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940426</creationdate><title>Erythropoietin Receptor mRNA Expression in Human Endothelial Cells</title><author>Anagnostou, Athanasius ; Liu, Ziyao ; Steiner, Manfred ; Chin, Kyung ; Lee, Eun S. ; Kessimian, Noubar ; Noguchi, Constance T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5254-2fc464251132d8d537856ecde1672c38d2dc3ae31e4a1ba4ac90527b592d447a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell lines</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Complementary DNA</topic><topic>DNA Primers - chemistry</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - physiology</topic><topic>Erythroid cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>HeLa cells</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>Human umbilical vein endothelial cells</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>In Vitro Techniques</topic><topic>K562 cells</topic><topic>Medical research</topic><topic>Messenger RNA</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Polymerase chain reaction</topic><topic>Receptors, Erythropoietin - genetics</topic><topic>Receptors, Erythropoietin - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anagnostou, Athanasius</creatorcontrib><creatorcontrib>Liu, Ziyao</creatorcontrib><creatorcontrib>Steiner, Manfred</creatorcontrib><creatorcontrib>Chin, Kyung</creatorcontrib><creatorcontrib>Lee, Eun S.</creatorcontrib><creatorcontrib>Kessimian, Noubar</creatorcontrib><creatorcontrib>Noguchi, Constance T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anagnostou, Athanasius</au><au>Liu, Ziyao</au><au>Steiner, Manfred</au><au>Chin, Kyung</au><au>Lee, Eun S.</au><au>Kessimian, Noubar</au><au>Noguchi, Constance T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Erythropoietin Receptor mRNA Expression in Human Endothelial Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-04-26</date><risdate>1994</risdate><volume>91</volume><issue>9</issue><spage>3974</spage><epage>3978</epage><pages>3974-3978</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A previous report demonstrated that endothelial cells have erythropoietin receptors and respond to this hormone with enhanced proliferation. The present study demonstrates the existence of mRNA for erythropoietin receptor in human umbilical vein endothelial cells. We have reverse transcribed mRNA of endothelial cells and then used different PCR primers to amplify erythropoietin receptor target cDNA between exons 5 and 6 as well as 3-5 in addition to an internal standard DNA fragment. Correspondence of size as well as location of restriction endonuclease scission (Ava II) was used in comparing the amplified fragments of human endothelial cell erythropoietin receptor to those of two human erythroleukemia cell lines, OCIM1 and K562. No α- or γ-globin mRNA was detected in endothelial cells but was readily demonstrable in OCIM1 cells. In addition, to determine whether the expression of human erythropoietin receptor on endothelial cells occurs in vivo, sections of umbilical cord and placenta were immunostained with antibodies against the extracellular portion of the receptor; the results showed strong positive staining of the vascular endothelium</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8171022</pmid><doi>10.1073/pnas.91.9.3974</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-04, Vol.91 (9), p.3974-3978 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Biochemistry Biological and medical sciences Cell lines Cell receptors Cell structures and functions Complementary DNA DNA Primers - chemistry Endothelial cells Endothelium, Vascular - physiology Erythroid cells Fundamental and applied biological sciences. Psychology Gene Expression HeLa cells Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors Human umbilical vein endothelial cells Humans Immunoenzyme Techniques In Vitro Techniques K562 cells Medical research Messenger RNA Molecular and cellular biology Molecular Sequence Data Polymerase chain reaction Receptors, Erythropoietin - genetics Receptors, Erythropoietin - metabolism Ribonucleic acid RNA RNA, Messenger - genetics Umbilical cord |
| title | Erythropoietin Receptor mRNA Expression in Human Endothelial Cells |
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