Bud break and multiple shoot formation from tissues of mature trees of Pinus caribaea and Pinus kesiya
Proliferation of terminal and axillary buds of 20 yr-old Pinus caribaea and P. kesiya trees was obtained on half strength DCR medium supplemented with$0.5 mg \cdot liter^{-1}$6-benzylaminopurine (BA). These sprouts further elongated with the formation of multiple shoots with the ratio of 1:3, on tra...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 1993-07, Vol.29P (3), p.131-134 |
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| container_title | In vitro cellular & developmental biology. Plant |
| container_volume | 29P |
| creator | Nadgauda, R.S Nagarwala, N.N Parasharami, V.A Mascarenhas, A.F |
| description | Proliferation of terminal and axillary buds of 20 yr-old Pinus caribaea and P. kesiya trees was obtained on half strength DCR medium supplemented with$0.5 mg \cdot liter^{-1}$6-benzylaminopurine (BA). These sprouts further elongated with the formation of multiple shoots with the ratio of 1:3, on transfer to medium in which the$0.25 mg \cdot liter^{-1} BA$of the initiation medium was replaced by$0.25 mg \cdot liter^{-1}$kinetin. Rooting was obtained on the same medium. Plantlets thus formed were transferred to perlite:peat:vermiculite mixture (1:1:1) in polybags (10 x 5 cm) under$80 \pm 5\%$humidity in a polyhouse. Plantlets of P. caribaea and P. kesiya were established with 72.5 and 83.3% survival, respectively. |
| doi_str_mv | 10.1007/BF02632284 |
| format | Article |
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These sprouts further elongated with the formation of multiple shoots with the ratio of 1:3, on transfer to medium in which the$0.25 mg \cdot liter^{-1} BA$of the initiation medium was replaced by$0.25 mg \cdot liter^{-1}$kinetin. Rooting was obtained on the same medium. Plantlets thus formed were transferred to perlite:peat:vermiculite mixture (1:1:1) in polybags (10 x 5 cm) under$80 \pm 5\%$humidity in a polyhouse. Plantlets of P. caribaea and P. kesiya were established with 72.5 and 83.3% survival, respectively.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02632284</identifier><language>eng</language><publisher>Wallingford: Tissue Culture Association, Inc</publisher><subject>6-BENZILADENINA ; 6-BENZYLADENINE ; Agronomy. 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Plant</title><description>Proliferation of terminal and axillary buds of 20 yr-old Pinus caribaea and P. kesiya trees was obtained on half strength DCR medium supplemented with$0.5 mg \cdot liter^{-1}$6-benzylaminopurine (BA). These sprouts further elongated with the formation of multiple shoots with the ratio of 1:3, on transfer to medium in which the$0.25 mg \cdot liter^{-1} BA$of the initiation medium was replaced by$0.25 mg \cdot liter^{-1}$kinetin. Rooting was obtained on the same medium. Plantlets thus formed were transferred to perlite:peat:vermiculite mixture (1:1:1) in polybags (10 x 5 cm) under$80 \pm 5\%$humidity in a polyhouse. Plantlets of P. caribaea and P. kesiya were established with 72.5 and 83.3% survival, respectively.</description><subject>6-BENZILADENINA</subject><subject>6-BENZYLADENINE</subject><subject>Agronomy. Soil science and plant productions</subject><subject>BENZYLADENINE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>BOURGEON</subject><subject>BUDS</subject><subject>Coniferous forests</subject><subject>Conifers</subject><subject>CULTIVO DE TEJIDOS</subject><subject>CULTURE DE TISSU</subject><subject>CULTURE MEDIA</subject><subject>EXPLANT</subject><subject>EXPLANTES</subject><subject>EXPLANTS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IN VITRO REGENERATION</subject><subject>Indian culture</subject><subject>KINETIN</subject><subject>KINETINE</subject><subject>MEDIO DE CULTIVO</subject><subject>MICROPROPAGACION</subject><subject>MICROPROPAGATION</subject><subject>MILIEU DE CULTURE</subject><subject>ORGANOGENESE</subject><subject>ORGANOGENESIS</subject><subject>PINUS CARIBAEA</subject><subject>PINUS KESIYA</subject><subject>Plant cuttings</subject><subject>Plant physiology and development</subject><subject>Plant propagation</subject><subject>Plantlets</subject><subject>QUINETINA</subject><subject>REGENERACION IN VITRO</subject><subject>REGENERATION IN VITRO</subject><subject>REGENERATIVE ABILITY</subject><subject>Rooting</subject><subject>Sprouting</subject><subject>STEMS</subject><subject>TALLO</subject><subject>TIGE</subject><subject>TISSUE CULTURE</subject><subject>Tissue cultures, protoplasts</subject><subject>Trees</subject><subject>YEMA (PLANTA)</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNo9jDtPwzAUhS0EEqWwMCIGD6wBv-LHSCsKSJVAgs7VjWOD2ySu7GTovyciiOmee76jD6FrSu4pIephsSJMcsa0OEEzKlRZMKnN6ZhJKYpSKHmOLnLeEUIooWqG_GKocZUc7DF0NW6Hpg-HxuH8HWOPfUwt9CF22KfY4j7kPLiMo8djPSSH--Sm_z10Q8YWUqjAwa9rqvYuhyNcojMPTXZXf3eONqunz-VLsX57fl0-rgvPGOsLS2pQwnPJSuKVE5UnoK3RlHLrjTKlAKtLAMO5lKbidW1cLR0zpCIlA8bn6G7yHiBbaHyCzoa8PaTQQjpuuSZMKTnObqfZLvcx_WPBDDNaj_hmwh7iFr7SaNh8GEGN0oz_ANKVai0</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Nadgauda, R.S</creator><creator>Nagarwala, N.N</creator><creator>Parasharami, V.A</creator><creator>Mascarenhas, A.F</creator><general>Tissue Culture Association, Inc</general><general>Cambridge University Press</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>19930701</creationdate><title>Bud break and multiple shoot formation from tissues of mature trees of Pinus caribaea and Pinus kesiya</title><author>Nadgauda, R.S ; Nagarwala, N.N ; Parasharami, V.A ; Mascarenhas, A.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f222t-c0da74f36250f7e4bf0a8c98113cf97954ac85aa933669b3dd9ed6e290b052a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>6-BENZILADENINA</topic><topic>6-BENZYLADENINE</topic><topic>Agronomy. Soil science and plant productions</topic><topic>BENZYLADENINE</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>BOURGEON</topic><topic>BUDS</topic><topic>Coniferous forests</topic><topic>Conifers</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTURE DE TISSU</topic><topic>CULTURE MEDIA</topic><topic>EXPLANT</topic><topic>EXPLANTES</topic><topic>EXPLANTS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IN VITRO REGENERATION</topic><topic>Indian culture</topic><topic>KINETIN</topic><topic>KINETINE</topic><topic>MEDIO DE CULTIVO</topic><topic>MICROPROPAGACION</topic><topic>MICROPROPAGATION</topic><topic>MILIEU DE CULTURE</topic><topic>ORGANOGENESE</topic><topic>ORGANOGENESIS</topic><topic>PINUS CARIBAEA</topic><topic>PINUS KESIYA</topic><topic>Plant cuttings</topic><topic>Plant physiology and development</topic><topic>Plant propagation</topic><topic>Plantlets</topic><topic>QUINETINA</topic><topic>REGENERACION IN VITRO</topic><topic>REGENERATION IN VITRO</topic><topic>REGENERATIVE ABILITY</topic><topic>Rooting</topic><topic>Sprouting</topic><topic>STEMS</topic><topic>TALLO</topic><topic>TIGE</topic><topic>TISSUE CULTURE</topic><topic>Tissue cultures, protoplasts</topic><topic>Trees</topic><topic>YEMA (PLANTA)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nadgauda, R.S</creatorcontrib><creatorcontrib>Nagarwala, N.N</creatorcontrib><creatorcontrib>Parasharami, V.A</creatorcontrib><creatorcontrib>Mascarenhas, A.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nadgauda, R.S</au><au>Nagarwala, N.N</au><au>Parasharami, V.A</au><au>Mascarenhas, A.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bud break and multiple shoot formation from tissues of mature trees of Pinus caribaea and Pinus kesiya</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>1993-07-01</date><risdate>1993</risdate><volume>29P</volume><issue>3</issue><spage>131</spage><epage>134</epage><pages>131-134</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Proliferation of terminal and axillary buds of 20 yr-old Pinus caribaea and P. kesiya trees was obtained on half strength DCR medium supplemented with$0.5 mg \cdot liter^{-1}$6-benzylaminopurine (BA). These sprouts further elongated with the formation of multiple shoots with the ratio of 1:3, on transfer to medium in which the$0.25 mg \cdot liter^{-1} BA$of the initiation medium was replaced by$0.25 mg \cdot liter^{-1}$kinetin. Rooting was obtained on the same medium. Plantlets thus formed were transferred to perlite:peat:vermiculite mixture (1:1:1) in polybags (10 x 5 cm) under$80 \pm 5\%$humidity in a polyhouse. Plantlets of P. caribaea and P. kesiya were established with 72.5 and 83.3% survival, respectively.</abstract><cop>Wallingford</cop><pub>Tissue Culture Association, Inc</pub><doi>10.1007/BF02632284</doi><tpages>4</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 1993-07, Vol.29P (3), p.131-134 |
| issn | 1054-5476 1475-2689 |
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| source | Jstor Complete Legacy; Springer Nature - Complete Springer Journals |
| subjects | 6-BENZILADENINA 6-BENZYLADENINE Agronomy. Soil science and plant productions BENZYLADENINE Biological and medical sciences Biotechnology BOURGEON BUDS Coniferous forests Conifers CULTIVO DE TEJIDOS CULTURE DE TISSU CULTURE MEDIA EXPLANT EXPLANTES EXPLANTS Fundamental and applied biological sciences. Psychology IN VITRO REGENERATION Indian culture KINETIN KINETINE MEDIO DE CULTIVO MICROPROPAGACION MICROPROPAGATION MILIEU DE CULTURE ORGANOGENESE ORGANOGENESIS PINUS CARIBAEA PINUS KESIYA Plant cuttings Plant physiology and development Plant propagation Plantlets QUINETINA REGENERACION IN VITRO REGENERATION IN VITRO REGENERATIVE ABILITY Rooting Sprouting STEMS TALLO TIGE TISSUE CULTURE Tissue cultures, protoplasts Trees YEMA (PLANTA) |
| title | Bud break and multiple shoot formation from tissues of mature trees of Pinus caribaea and Pinus kesiya |
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