Probing the function of Drosophila melanogaster accessory glands by directed cell ablation
The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flie...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-09, Vol.90 (17), p.8093-8097 |
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| creator | Kalb, J.M DiBenedetto, A.J Wolfner, M.F |
| description | The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells. |
| doi_str_mv | 10.1073/pnas.90.17.8093 |
| format | Article |
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Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.17.8093</identifier><identifier>PMID: 8367469</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>ANIMAL TRANSGENIQUE ; ANIMALES TRANSGENICOS ; Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; COMPORTAMIENTO SEXUAL ; COMPORTEMENT SEXUEL ; Corynebacterium diphtheriae ; Crosses, Genetic ; Diphtheria Toxin - biosynthesis ; Drosophila ; DROSOPHILA MELANOGASTER ; Drosophila melanogaster - cytology ; Drosophila melanogaster - genetics ; Drosophila melanogaster - physiology ; Female ; Female animals ; FEMELLE ; FONCTION PHYSIOLOGIQUE ; FUNCION FISIOLOGICA ; Fundamental and applied biological sciences. Psychology ; Genetics ; GENIE GENETIQUE ; GLANDE ANIMALE ; GLANDULAS ANIMALES ; HEMBRA ; INGENIERIA GENETICA ; Insect genetics ; Insecta ; Insects ; Invertebrates ; MACHO ; MALE ; Male animals ; Mating behavior ; Medical research ; Ova ; Oviposition ; Physiology. Development ; Promoter Regions, Genetic ; Protein Biosynthesis ; REPRODUCCION ; REPRODUCTION ; Respiratory diseases ; Restriction Mapping ; Sebaceous Glands - cytology ; Sebaceous Glands - physiology ; SECRECION ; SECRETION ; Spermatozoa ; Spermatozoa - physiology ; Testis - physiology ; TOXINAS ; TOXINE ; Toxins</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-09, Vol.90 (17), p.8093-8097</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Sep 1, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c634t-ed52ebb635c38fc54b24f5edea1903adfa2e0693b9d3c269a6d8340a08dfede13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/17.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2362971$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2362971$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27931,27932,53798,53800,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3764025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8367469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalb, J.M</creatorcontrib><creatorcontrib>DiBenedetto, A.J</creatorcontrib><creatorcontrib>Wolfner, M.F</creatorcontrib><title>Probing the function of Drosophila melanogaster accessory glands by directed cell ablation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells.</description><subject>ANIMAL TRANSGENIQUE</subject><subject>ANIMALES TRANSGENICOS</subject><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>COMPORTAMIENTO SEXUAL</subject><subject>COMPORTEMENT SEXUEL</subject><subject>Corynebacterium diphtheriae</subject><subject>Crosses, Genetic</subject><subject>Diphtheria Toxin - biosynthesis</subject><subject>Drosophila</subject><subject>DROSOPHILA MELANOGASTER</subject><subject>Drosophila melanogaster - cytology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - physiology</subject><subject>Female</subject><subject>Female animals</subject><subject>FEMELLE</subject><subject>FONCTION PHYSIOLOGIQUE</subject><subject>FUNCION FISIOLOGICA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>GENIE GENETIQUE</subject><subject>GLANDE ANIMALE</subject><subject>GLANDULAS ANIMALES</subject><subject>HEMBRA</subject><subject>INGENIERIA GENETICA</subject><subject>Insect genetics</subject><subject>Insecta</subject><subject>Insects</subject><subject>Invertebrates</subject><subject>MACHO</subject><subject>MALE</subject><subject>Male animals</subject><subject>Mating behavior</subject><subject>Medical research</subject><subject>Ova</subject><subject>Oviposition</subject><subject>Physiology. Development</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Biosynthesis</subject><subject>REPRODUCCION</subject><subject>REPRODUCTION</subject><subject>Respiratory diseases</subject><subject>Restriction Mapping</subject><subject>Sebaceous Glands - cytology</subject><subject>Sebaceous Glands - physiology</subject><subject>SECRECION</subject><subject>SECRETION</subject><subject>Spermatozoa</subject><subject>Spermatozoa - physiology</subject><subject>Testis - physiology</subject><subject>TOXINAS</subject><subject>TOXINE</subject><subject>Toxins</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhhtR1nH1LIhKENFTz-ar0x3wIusnLCjoXryE6nTS00MmmU26xfn3ppl2cD14CpX3qaq3qoriMcFrgmt2sfeQ1jIH9brBkt0pVgRLUgou8d1ihTGty4ZTfr94kNIWYyyrBp8VZw0TNRdyVfz4GkM7-B6NG4Ps5PU4BI-CRe9iSGG_GRygnXHgQw9pNBGB1ialEA-oz79dQu0BdUM0ejQd0sY5BK2DucrD4p4Fl8yj5T0vrj-8_375qbz68vHz5durUgvGx9J0FTVtK1ilWWN1xVvKbWU6A0RiBp0FarCQrJUd01RIEF3DOAbcdDZThJ0Xb45191O7M502fozg1D4OO4gHFWBQtxU_bFQffipeU8lz-qslPYabyaRR7YY0DwLehCkpIkT2VtEMvvgH3IYp-jyaopgwQStRZejiCOm8vxSNPfkgWM0XU_PFlMxBreaL5Yxnf9s_8cuJsv5y0SFpcDaC10M6YawWHNO58fMFm-v_UW_1ef1fQNnJudH8GjP59Ehu0xjiCaV5RFnP-35ylC0EBX3MZq6_5UUS3FTsN116yvg</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Kalb, J.M</creator><creator>DiBenedetto, A.J</creator><creator>Wolfner, M.F</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19930901</creationdate><title>Probing the function of Drosophila melanogaster accessory glands by directed cell ablation</title><author>Kalb, J.M ; DiBenedetto, A.J ; Wolfner, M.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c634t-ed52ebb635c38fc54b24f5edea1903adfa2e0693b9d3c269a6d8340a08dfede13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ANIMAL TRANSGENIQUE</topic><topic>ANIMALES TRANSGENICOS</topic><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>COMPORTAMIENTO SEXUAL</topic><topic>COMPORTEMENT SEXUEL</topic><topic>Corynebacterium diphtheriae</topic><topic>Crosses, Genetic</topic><topic>Diphtheria Toxin - biosynthesis</topic><topic>Drosophila</topic><topic>DROSOPHILA MELANOGASTER</topic><topic>Drosophila melanogaster - cytology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - physiology</topic><topic>Female</topic><topic>Female animals</topic><topic>FEMELLE</topic><topic>FONCTION PHYSIOLOGIQUE</topic><topic>FUNCION FISIOLOGICA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>GENIE GENETIQUE</topic><topic>GLANDE ANIMALE</topic><topic>GLANDULAS ANIMALES</topic><topic>HEMBRA</topic><topic>INGENIERIA GENETICA</topic><topic>Insect genetics</topic><topic>Insecta</topic><topic>Insects</topic><topic>Invertebrates</topic><topic>MACHO</topic><topic>MALE</topic><topic>Male animals</topic><topic>Mating behavior</topic><topic>Medical research</topic><topic>Ova</topic><topic>Oviposition</topic><topic>Physiology. Development</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Biosynthesis</topic><topic>REPRODUCCION</topic><topic>REPRODUCTION</topic><topic>Respiratory diseases</topic><topic>Restriction Mapping</topic><topic>Sebaceous Glands - cytology</topic><topic>Sebaceous Glands - physiology</topic><topic>SECRECION</topic><topic>SECRETION</topic><topic>Spermatozoa</topic><topic>Spermatozoa - physiology</topic><topic>Testis - physiology</topic><topic>TOXINAS</topic><topic>TOXINE</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalb, J.M</creatorcontrib><creatorcontrib>DiBenedetto, A.J</creatorcontrib><creatorcontrib>Wolfner, M.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalb, J.M</au><au>DiBenedetto, A.J</au><au>Wolfner, M.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probing the function of Drosophila melanogaster accessory glands by directed cell ablation</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>90</volume><issue>17</issue><spage>8093</spage><epage>8097</epage><pages>8093-8097</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8367469</pmid><doi>10.1073/pnas.90.17.8093</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1993-09, Vol.90 (17), p.8093-8097 |
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| subjects | ANIMAL TRANSGENIQUE ANIMALES TRANSGENICOS Animals Biochemistry. Physiology. Immunology Biological and medical sciences COMPORTAMIENTO SEXUAL COMPORTEMENT SEXUEL Corynebacterium diphtheriae Crosses, Genetic Diphtheria Toxin - biosynthesis Drosophila DROSOPHILA MELANOGASTER Drosophila melanogaster - cytology Drosophila melanogaster - genetics Drosophila melanogaster - physiology Female Female animals FEMELLE FONCTION PHYSIOLOGIQUE FUNCION FISIOLOGICA Fundamental and applied biological sciences. Psychology Genetics GENIE GENETIQUE GLANDE ANIMALE GLANDULAS ANIMALES HEMBRA INGENIERIA GENETICA Insect genetics Insecta Insects Invertebrates MACHO MALE Male animals Mating behavior Medical research Ova Oviposition Physiology. Development Promoter Regions, Genetic Protein Biosynthesis REPRODUCCION REPRODUCTION Respiratory diseases Restriction Mapping Sebaceous Glands - cytology Sebaceous Glands - physiology SECRECION SECRETION Spermatozoa Spermatozoa - physiology Testis - physiology TOXINAS TOXINE Toxins |
| title | Probing the function of Drosophila melanogaster accessory glands by directed cell ablation |
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