Cloning and Nucleotide Sequence of the Gene Responsible for Chlorination of Tetracycline

Two cosmid clones containing distinct types of self-defense gene of a 6-demethylchlortetracycline producer, Streptomyces aureofaciens NRRL3203, were isolated. The gene responsible for chlorination of tetracycline (chl gene) was subcloned from one of the cosmid clones by complementation of a chlorina...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (6), p.1099-1106
Hauptverfasser:	Dairi, Tohru, Nakano, Tetsuo, Aisaka, Kazuo, Katsumata, Ryoichl, Hasegawa, Mamoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Chlorine - metabolism Cloning, Molecular Cosmids Demeclocycline - metabolism DNA, Recombinant Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Complementation Test Genetics Microbiology Molecular Sequence Data Sequence Homology, Amino Acid Streptomyces aureofaciens - genetics Streptomyces aureofaciens - metabolism Tetracycline - metabolism Transcription, Genetic
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