Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine
A bacterium that was capable of metabolizing atrazine at very high concentrations ( 1,000 ppm) was isolated from a herbicide spill site. The organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. Detailed taxonomic studies identified the organ...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1995-04, Vol.61 (4), p.1451-1457 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Mandelbaum, R.T. (Volcani Research Center, Bet-Dagan, Israel) Allan, D.L Wackett, L.P |
| description | A bacterium that was capable of metabolizing atrazine at very high concentrations ( 1,000 ppm) was isolated from a herbicide spill site. The organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. Detailed taxonomic studies identified the organism as a Pseudomonas sp., designated ADP, that was dissimilar to currently known species. Pseudomonas sp. strain ADP metabolized atrazine as its sole nitrogen source. Nongrowing suspended cells also metabolized atrazine rapidly; for example, 9 X 10(9) cells per ml degraded 100 ppm of atrazine in 90 min. Atrazine was metabolized to hydroxyatrazine, polar metabolites, and carbon dioxide. When uniformly ring-labeled [14C]atrazine was used, 80% of the radioactivity was liberated as 14CO2. These data indicated the triazine ring was completely mineralized. The isolation and characterization of Pseudomonas sp. strain ADP may contribute to efforts on atrazine bioremediation, particularly in environments containing very high pesticide levels |
| doi_str_mv | 10.1128/AEM.61.4.1451-1457.1995 |
| format | Article |
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(Volcani Research Center, Bet-Dagan, Israel) ; Allan, D.L ; Wackett, L.P</creator><creatorcontrib>Mandelbaum, R.T. (Volcani Research Center, Bet-Dagan, Israel) ; Allan, D.L ; Wackett, L.P</creatorcontrib><description>A bacterium that was capable of metabolizing atrazine at very high concentrations ( 1,000 ppm) was isolated from a herbicide spill site. The organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. Detailed taxonomic studies identified the organism as a Pseudomonas sp., designated ADP, that was dissimilar to currently known species. Pseudomonas sp. strain ADP metabolized atrazine as its sole nitrogen source. Nongrowing suspended cells also metabolized atrazine rapidly; for example, 9 X 10(9) cells per ml degraded 100 ppm of atrazine in 90 min. Atrazine was metabolized to hydroxyatrazine, polar metabolites, and carbon dioxide. When uniformly ring-labeled [14C]atrazine was used, 80% of the radioactivity was liberated as 14CO2. These data indicated the triazine ring was completely mineralized. The isolation and characterization of Pseudomonas sp. strain ADP may contribute to efforts on atrazine bioremediation, particularly in environments containing very high pesticide levels</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.61.4.1451-1457.1995</identifier><identifier>PMID: 16534995</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ATRAZINA ; ATRAZINE ; BACTERIA ; BIODEGRADACION ; BIODEGRADATION ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Classification ; FLORA DEL SUELO ; FLORE DU SOL ; Fundamental and applied biological sciences. Psychology ; Herbicides ; Isolation and description ; MINERALISATION ; MINERALIZACION ; Mission oriented research ; POLLUTION DU SOL ; POLUCION DEL SUELO ; PSEUDOMONAS ; SOL ANTHROPOGENE ; SUELOS ANTROPOGENICOS</subject><ispartof>Applied and Environmental Microbiology, 1995-04, Vol.61 (4), p.1451-1457</ispartof><rights>1995 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Apr 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c600t-ea56e91efffb9cf2a4bc759aa029693602e8ccc6573e5bc21afabf74716fd5ac3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388413/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388413/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3179,3180,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3499198$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16534995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mandelbaum, R.T. (Volcani Research Center, Bet-Dagan, Israel)</creatorcontrib><creatorcontrib>Allan, D.L</creatorcontrib><creatorcontrib>Wackett, L.P</creatorcontrib><title>Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A bacterium that was capable of metabolizing atrazine at very high concentrations ( 1,000 ppm) was isolated from a herbicide spill site. The organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. Detailed taxonomic studies identified the organism as a Pseudomonas sp., designated ADP, that was dissimilar to currently known species. Pseudomonas sp. strain ADP metabolized atrazine as its sole nitrogen source. Nongrowing suspended cells also metabolized atrazine rapidly; for example, 9 X 10(9) cells per ml degraded 100 ppm of atrazine in 90 min. 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(Volcani Research Center, Bet-Dagan, Israel) ; Allan, D.L ; Wackett, L.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-ea56e91efffb9cf2a4bc759aa029693602e8ccc6573e5bc21afabf74716fd5ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ATRAZINA</topic><topic>ATRAZINE</topic><topic>BACTERIA</topic><topic>BIODEGRADACION</topic><topic>BIODEGRADATION</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Classification</topic><topic>FLORA DEL SUELO</topic><topic>FLORE DU SOL</topic><topic>Fundamental and applied biological sciences. 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(Volcani Research Center, Bet-Dagan, Israel)</au><au>Allan, D.L</au><au>Wackett, L.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>61</volume><issue>4</issue><spage>1451</spage><epage>1457</epage><pages>1451-1457</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A bacterium that was capable of metabolizing atrazine at very high concentrations ( 1,000 ppm) was isolated from a herbicide spill site. The organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. Detailed taxonomic studies identified the organism as a Pseudomonas sp., designated ADP, that was dissimilar to currently known species. 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| ispartof | Applied and Environmental Microbiology, 1995-04, Vol.61 (4), p.1451-1457 |
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| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | ATRAZINA ATRAZINE BACTERIA BIODEGRADACION BIODEGRADATION Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Classification FLORA DEL SUELO FLORE DU SOL Fundamental and applied biological sciences. Psychology Herbicides Isolation and description MINERALISATION MINERALIZACION Mission oriented research POLLUTION DU SOL POLUCION DEL SUELO PSEUDOMONAS SOL ANTHROPOGENE SUELOS ANTROPOGENICOS |
| title | Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine |
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