rRNA gene restriction patterns of Streptococcus pyogenes: epidemiological applications and relation to serotypes
The use of rRNA gene restriction fragment length polymorphism analysis (ribotyping) to subtype Streptococcus pyogenes strains was investigated. Sixty-eight S. pyogenes strains, including 17 reference strains and 51 isolates from blood, acute or chronic pharyngitis, and food-borne outbreaks, were cha...
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| Veröffentlicht in: | Journal of Clinical Microbiology 1994-12, Vol.32 (12), p.2953-2958 |
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| creator | Bruneau, S. (Institut Pasteur de Lyon, Lyon, France.) Montclos, H. de Drouet, E Denoyel, G.A |
| description | The use of rRNA gene restriction fragment length polymorphism analysis (ribotyping) to subtype Streptococcus pyogenes strains was investigated. Sixty-eight S. pyogenes strains, including 17 reference strains and 51 isolates from blood, acute or chronic pharyngitis, and food-borne outbreaks, were characterized by determination of both their rDNA restriction fragment length polymorphism profiles and their serotypes (T and M). Total DNA was cleaved with five selected restriction enzymes and then probed with a digoxigenin-labeled stretch of 1,063 bp hybridizing with 16S rRNA genes. Fifteen and nine distinct patterns were generated with SacI and XhoI, respectively, and five patterns were generated with each of the three additional restriction enzymes. With the combination SacI-XhoI, a total of 21 distinct ribotypes were obtained among the 68 isolates. This number was not increased by the results obtained with the other restriction enzymes. All strains tested were typeable. All isolates from each food-borne outbreak belonged to the same ribotype, and all isolates (pre-and posttreatment) from each child with chronic pharyngitis also belonged to the same ribotype, suggesting antibiotic treatment failures. A discriminatory index was calculated for the 47 isolates which were epidemiologically unrelated, using the Hunter-Gaston formula. This index reached 0.955 when the combination SacI-XhoI was used, showing the good discriminatory power of this typing method. Therefore, ribotyping proved to be a molecular method of interest to subtype S. pyogenes. Moreover, there was some correlation between ribotyping and serotyping, as several ribotypes were related to a unique distinct M serotype |
| doi_str_mv | 10.1128/jcm.32.12.2953-2958.1994 |
| format | Article |
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(Institut Pasteur de Lyon, Lyon, France.) ; Montclos, H. de ; Drouet, E ; Denoyel, G.A</creator><creatorcontrib>Bruneau, S. (Institut Pasteur de Lyon, Lyon, France.) ; Montclos, H. de ; Drouet, E ; Denoyel, G.A</creatorcontrib><description>The use of rRNA gene restriction fragment length polymorphism analysis (ribotyping) to subtype Streptococcus pyogenes strains was investigated. Sixty-eight S. pyogenes strains, including 17 reference strains and 51 isolates from blood, acute or chronic pharyngitis, and food-borne outbreaks, were characterized by determination of both their rDNA restriction fragment length polymorphism profiles and their serotypes (T and M). Total DNA was cleaved with five selected restriction enzymes and then probed with a digoxigenin-labeled stretch of 1,063 bp hybridizing with 16S rRNA genes. Fifteen and nine distinct patterns were generated with SacI and XhoI, respectively, and five patterns were generated with each of the three additional restriction enzymes. With the combination SacI-XhoI, a total of 21 distinct ribotypes were obtained among the 68 isolates. This number was not increased by the results obtained with the other restriction enzymes. All strains tested were typeable. All isolates from each food-borne outbreak belonged to the same ribotype, and all isolates (pre-and posttreatment) from each child with chronic pharyngitis also belonged to the same ribotype, suggesting antibiotic treatment failures. A discriminatory index was calculated for the 47 isolates which were epidemiologically unrelated, using the Hunter-Gaston formula. This index reached 0.955 when the combination SacI-XhoI was used, showing the good discriminatory power of this typing method. Therefore, ribotyping proved to be a molecular method of interest to subtype S. pyogenes. Moreover, there was some correlation between ribotyping and serotyping, as several ribotypes were related to a unique distinct M serotype</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.32.12.2953-2958.1994</identifier><identifier>PMID: 7533778</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ADN ; AFFECTION BUCCALE ; ARN RIBOSOMAL ; ARN RIBOSOMIAL ; Bacteriology ; Biological and medical sciences ; Child ; ENFERMEDADES DE LA BOCA ; ENFERMEDADES TRANSM POR ALIMENTOS ; EPIDEMIOLOGIA ; EPIDEMIOLOGIE ; ETATS UNIS ; EUA ; Foodborne Diseases - epidemiology ; Foodborne Diseases - microbiology ; FRANCE ; FRANCIA ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Humans ; IDENTIFICACION ; IDENTIFICATION ; MALADIE TRANSMISSIBLE PAR ALIMENT ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Pharyngitis - epidemiology ; Pharyngitis - microbiology ; POLIMORFISMO ; Polymorphism, Restriction Fragment Length ; POLYMORPHISME ; REPUBLICA CHECA ; REPUBLIQUE TCHEQUE ; RFCS ; RFTS ; RIBOSOMAS ; RIBOSOME ; RNA, Bacterial - genetics ; RNA, Ribosomal - genetics ; SEROTIPOS ; SEROTYPE ; Serotyping ; Streptococcal Infections - epidemiology ; Streptococcal Infections - microbiology ; STREPTOCOCCUS ; Streptococcus pyogenes ; Streptococcus pyogenes - classification ; Streptococcus pyogenes - genetics ; Streptococcus pyogenes - isolation & purification</subject><ispartof>Journal of Clinical Microbiology, 1994-12, Vol.32 (12), p.2953-2958</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4904-8a6c8edb9a5921cc7a317347382e8aaa07b59e22c86ef140b5a825250c1f02b73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC264206/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC264206/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,3175,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3340715$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7533778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bruneau, S. (Institut Pasteur de Lyon, Lyon, France.)</creatorcontrib><creatorcontrib>Montclos, H. de</creatorcontrib><creatorcontrib>Drouet, E</creatorcontrib><creatorcontrib>Denoyel, G.A</creatorcontrib><title>rRNA gene restriction patterns of Streptococcus pyogenes: epidemiological applications and relation to serotypes</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The use of rRNA gene restriction fragment length polymorphism analysis (ribotyping) to subtype Streptococcus pyogenes strains was investigated. Sixty-eight S. pyogenes strains, including 17 reference strains and 51 isolates from blood, acute or chronic pharyngitis, and food-borne outbreaks, were characterized by determination of both their rDNA restriction fragment length polymorphism profiles and their serotypes (T and M). Total DNA was cleaved with five selected restriction enzymes and then probed with a digoxigenin-labeled stretch of 1,063 bp hybridizing with 16S rRNA genes. Fifteen and nine distinct patterns were generated with SacI and XhoI, respectively, and five patterns were generated with each of the three additional restriction enzymes. With the combination SacI-XhoI, a total of 21 distinct ribotypes were obtained among the 68 isolates. This number was not increased by the results obtained with the other restriction enzymes. All strains tested were typeable. All isolates from each food-borne outbreak belonged to the same ribotype, and all isolates (pre-and posttreatment) from each child with chronic pharyngitis also belonged to the same ribotype, suggesting antibiotic treatment failures. A discriminatory index was calculated for the 47 isolates which were epidemiologically unrelated, using the Hunter-Gaston formula. This index reached 0.955 when the combination SacI-XhoI was used, showing the good discriminatory power of this typing method. Therefore, ribotyping proved to be a molecular method of interest to subtype S. pyogenes. Moreover, there was some correlation between ribotyping and serotyping, as several ribotypes were related to a unique distinct M serotype</description><subject>ADN</subject><subject>AFFECTION BUCCALE</subject><subject>ARN RIBOSOMAL</subject><subject>ARN RIBOSOMIAL</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>ENFERMEDADES DE LA BOCA</subject><subject>ENFERMEDADES TRANSM POR ALIMENTOS</subject><subject>EPIDEMIOLOGIA</subject><subject>EPIDEMIOLOGIE</subject><subject>ETATS UNIS</subject><subject>EUA</subject><subject>Foodborne Diseases - epidemiology</subject><subject>Foodborne Diseases - microbiology</subject><subject>FRANCE</subject><subject>FRANCIA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Humans</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>MALADIE TRANSMISSIBLE PAR ALIMENT</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Pharyngitis - epidemiology</subject><subject>Pharyngitis - microbiology</subject><subject>POLIMORFISMO</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>POLYMORPHISME</subject><subject>REPUBLICA CHECA</subject><subject>REPUBLIQUE TCHEQUE</subject><subject>RFCS</subject><subject>RFTS</subject><subject>RIBOSOMAS</subject><subject>RIBOSOME</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal - genetics</subject><subject>SEROTIPOS</subject><subject>SEROTYPE</subject><subject>Serotyping</subject><subject>Streptococcal Infections - epidemiology</subject><subject>Streptococcal Infections - microbiology</subject><subject>STREPTOCOCCUS</subject><subject>Streptococcus pyogenes</subject><subject>Streptococcus pyogenes - classification</subject><subject>Streptococcus pyogenes - genetics</subject><subject>Streptococcus pyogenes - isolation & purification</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV1rFDEUhoModVv9A4IQRHo3Yz4mk0TwohS_oChYC96FM9nMbsrMJE1mlf33ZtxlUW9OEs7zviecFyFMSU0pU2_u7VhzVlNWMy14VYqqqdbNI7SiRKuqbcmPx2hFiBYVpVw-Rec53xNCm0aIM3QmBedSqhWK6duXK7xxk8PJ5Tl5O_sw4Qjz7NKUcejx7ZxcnIMN1u4yjvuw0PktdtGv3ejDEDbewoAhxqFcFn3GMK2L4fDnheeAs0th3keXn6EnPQzZPT-eF-juw_vv15-qm68fP19f3VS20aSpFLRWuXWnQWhGrZXAqeSN5Io5BQBEdkI7xqxqXU8b0glQTDBBLO0J6yS_QO8OvnHXjW5t3TQnGExMfoS0NwG8-bcz-a3ZhJ-GtQ0jbdFfHvUpPOzKaszos3XDAJMLu2xoWTGVlBdQHUCbQs7J9acZlJglLFPCMpwZyswS1lKUWcIq0pd___EkPKZT-q-Pfchlw32Cyfp8wjhviKSiYK8O2NZvtr98cgby-N_UAr04QD0EA5tUfO5utRCMsob_Br9StT8</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>Bruneau, S. 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Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Humans</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>MALADIE TRANSMISSIBLE PAR ALIMENT</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Pharyngitis - epidemiology</topic><topic>Pharyngitis - microbiology</topic><topic>POLIMORFISMO</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>POLYMORPHISME</topic><topic>REPUBLICA CHECA</topic><topic>REPUBLIQUE TCHEQUE</topic><topic>RFCS</topic><topic>RFTS</topic><topic>RIBOSOMAS</topic><topic>RIBOSOME</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal - genetics</topic><topic>SEROTIPOS</topic><topic>SEROTYPE</topic><topic>Serotyping</topic><topic>Streptococcal Infections - epidemiology</topic><topic>Streptococcal Infections - microbiology</topic><topic>STREPTOCOCCUS</topic><topic>Streptococcus pyogenes</topic><topic>Streptococcus pyogenes - classification</topic><topic>Streptococcus pyogenes - genetics</topic><topic>Streptococcus pyogenes - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruneau, S. (Institut Pasteur de Lyon, Lyon, France.)</creatorcontrib><creatorcontrib>Montclos, H. de</creatorcontrib><creatorcontrib>Drouet, E</creatorcontrib><creatorcontrib>Denoyel, G.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruneau, S. (Institut Pasteur de Lyon, Lyon, France.)</au><au>Montclos, H. de</au><au>Drouet, E</au><au>Denoyel, G.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>rRNA gene restriction patterns of Streptococcus pyogenes: epidemiological applications and relation to serotypes</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>32</volume><issue>12</issue><spage>2953</spage><epage>2958</epage><pages>2953-2958</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>The use of rRNA gene restriction fragment length polymorphism analysis (ribotyping) to subtype Streptococcus pyogenes strains was investigated. Sixty-eight S. pyogenes strains, including 17 reference strains and 51 isolates from blood, acute or chronic pharyngitis, and food-borne outbreaks, were characterized by determination of both their rDNA restriction fragment length polymorphism profiles and their serotypes (T and M). Total DNA was cleaved with five selected restriction enzymes and then probed with a digoxigenin-labeled stretch of 1,063 bp hybridizing with 16S rRNA genes. Fifteen and nine distinct patterns were generated with SacI and XhoI, respectively, and five patterns were generated with each of the three additional restriction enzymes. With the combination SacI-XhoI, a total of 21 distinct ribotypes were obtained among the 68 isolates. This number was not increased by the results obtained with the other restriction enzymes. All strains tested were typeable. All isolates from each food-borne outbreak belonged to the same ribotype, and all isolates (pre-and posttreatment) from each child with chronic pharyngitis also belonged to the same ribotype, suggesting antibiotic treatment failures. A discriminatory index was calculated for the 47 isolates which were epidemiologically unrelated, using the Hunter-Gaston formula. This index reached 0.955 when the combination SacI-XhoI was used, showing the good discriminatory power of this typing method. Therefore, ribotyping proved to be a molecular method of interest to subtype S. pyogenes. Moreover, there was some correlation between ribotyping and serotyping, as several ribotypes were related to a unique distinct M serotype</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>7533778</pmid><doi>10.1128/jcm.32.12.2953-2958.1994</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ADN AFFECTION BUCCALE ARN RIBOSOMAL ARN RIBOSOMIAL Bacteriology Biological and medical sciences Child ENFERMEDADES DE LA BOCA ENFERMEDADES TRANSM POR ALIMENTOS EPIDEMIOLOGIA EPIDEMIOLOGIE ETATS UNIS EUA Foodborne Diseases - epidemiology Foodborne Diseases - microbiology FRANCE FRANCIA Fundamental and applied biological sciences. Psychology GENE GENES Humans IDENTIFICACION IDENTIFICATION MALADIE TRANSMISSIBLE PAR ALIMENT Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Pharyngitis - epidemiology Pharyngitis - microbiology POLIMORFISMO Polymorphism, Restriction Fragment Length POLYMORPHISME REPUBLICA CHECA REPUBLIQUE TCHEQUE RFCS RFTS RIBOSOMAS RIBOSOME RNA, Bacterial - genetics RNA, Ribosomal - genetics SEROTIPOS SEROTYPE Serotyping Streptococcal Infections - epidemiology Streptococcal Infections - microbiology STREPTOCOCCUS Streptococcus pyogenes Streptococcus pyogenes - classification Streptococcus pyogenes - genetics Streptococcus pyogenes - isolation & purification |
| title | rRNA gene restriction patterns of Streptococcus pyogenes: epidemiological applications and relation to serotypes |
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