The relationship between electropermeabilization and growth phase in a suspension of yeast cells, Saccharomyces cerevisiae
We examined the relationship between growth phase and the initial field strength required for electropermeabilization of yeast cells in suspension. As the period of time for the culture was increased, the initial field strength necessary for electropermeabilization of the cells also increased and th...
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| Veröffentlicht in: | Nippon nōgei kagakukaishi 1996, Vol.70 (5), p.563-568 |
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| container_issue | 5 |
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| container_title | Nippon nōgei kagakukaishi |
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| creator | TATEBE, W MURAJI, M FUJII, T |
| description | We examined the relationship between growth phase and the initial field strength required for electropermeabilization of yeast cells in suspension. As the period of time for the culture was increased, the initial field strength necessary for electropermeabilization of the cells also increased and the slope of the graph of the number of perforations against field strength became smaller. These findings suggest a correlation between growth phase and membrane strength for electric pulses. In "decelerated growth phase", there may have been a greater overall increase in membrane strength than in the case of "exponential growth phase". In a further, "resealing" experiment, observations lead us to conclude that differences in the growth phase of Saccharomyces cerevisiae tended not to influence the rate of membrane resealing. |
| doi_str_mv | 10.1271/nogeikagaku1924.70.563 |
| format | Article |
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In a further, "resealing" experiment, observations lead us to conclude that differences in the growth phase of Saccharomyces cerevisiae tended not to influence the rate of membrane resealing.</description><identifier>ISSN: 0002-1407</identifier><identifier>DOI: 10.1271/nogeikagaku1924.70.563</identifier><identifier>CODEN: NNKKAA</identifier><language>jpn</language><publisher>Tokyo: Nippon Nōgei Kagakukai</publisher><subject>cells ; cellule ; celulas ; electroporacion ; electroporation ; growth period ; periode de croissance ; periodo de crecimiento ; saccharomyces cerevisiae</subject><ispartof>Nippon nōgei kagakukaishi, 1996, Vol.70 (5), p.563-568</ispartof><rights>1996 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c294t-8d3df84bd2851025383bc6f1b1c836543133ed9bd5258c127377e769eb0805d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3116312$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>TATEBE, W</creatorcontrib><creatorcontrib>MURAJI, M</creatorcontrib><creatorcontrib>FUJII, T</creatorcontrib><creatorcontrib>Technische Univ. Berlin (Germany). Inst. fuer Oekologie. Fachgebiete Bodenkunde/Standortkunde und Bodenschutz</creatorcontrib><creatorcontrib>Osaka City Univ. (Japan)</creatorcontrib><title>The relationship between electropermeabilization and growth phase in a suspension of yeast cells, Saccharomyces cerevisiae</title><title>Nippon nōgei kagakukaishi</title><description>We examined the relationship between growth phase and the initial field strength required for electropermeabilization of yeast cells in suspension. As the period of time for the culture was increased, the initial field strength necessary for electropermeabilization of the cells also increased and the slope of the graph of the number of perforations against field strength became smaller. These findings suggest a correlation between growth phase and membrane strength for electric pulses. In "decelerated growth phase", there may have been a greater overall increase in membrane strength than in the case of "exponential growth phase". In a further, "resealing" experiment, observations lead us to conclude that differences in the growth phase of Saccharomyces cerevisiae tended not to influence the rate of membrane resealing.</description><subject>cells</subject><subject>cellule</subject><subject>celulas</subject><subject>electroporacion</subject><subject>electroporation</subject><subject>growth period</subject><subject>periode de croissance</subject><subject>periodo de crecimiento</subject><subject>saccharomyces cerevisiae</subject><issn>0002-1407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNot0M1OwzAQBGAfQKIqfYXKB46keO04To6o4leVQKKcq42zSUzTJLJTqvbpCZTTaEef9jCMzUEsQBq4a7uK3BYr3O4hk_HCiIVO1AWbCCFkBLEwV2wWwtd4Cm2yRKoJO61r4p4aHFzXhtr1PKfhQNRyasgOvuvJ7whz17jTn-HYFrzy3WGoeV9jIO7Gjod96KkNv6Ar-ZEwDNxS04Rb_oHW1ui73dFSGEtP3y44pGt2WWITaPafU_b5-LBePkert6eX5f0qsjKLhygtVFGmcV7IVIOQWqUqt0kJOdhUJTpWoBQVWV5oqVM7DqGMIZNklItU6ALUlN2c__YYLDalx9a6sOm926E_bhRAokCObH5mJXYbrPxIXt8hy4yAOJEiVj-jCGym</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>TATEBE, W</creator><creator>MURAJI, M</creator><creator>FUJII, T</creator><general>Nippon Nōgei Kagakukai</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>1996</creationdate><title>The relationship between electropermeabilization and growth phase in a suspension of yeast cells, Saccharomyces cerevisiae</title><author>TATEBE, W ; MURAJI, M ; FUJII, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c294t-8d3df84bd2851025383bc6f1b1c836543133ed9bd5258c127377e769eb0805d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>1996</creationdate><topic>cells</topic><topic>cellule</topic><topic>celulas</topic><topic>electroporacion</topic><topic>electroporation</topic><topic>growth period</topic><topic>periode de croissance</topic><topic>periodo de crecimiento</topic><topic>saccharomyces cerevisiae</topic><toplevel>online_resources</toplevel><creatorcontrib>TATEBE, W</creatorcontrib><creatorcontrib>MURAJI, M</creatorcontrib><creatorcontrib>FUJII, T</creatorcontrib><creatorcontrib>Technische Univ. Berlin (Germany). Inst. fuer Oekologie. Fachgebiete Bodenkunde/Standortkunde und Bodenschutz</creatorcontrib><creatorcontrib>Osaka City Univ. (Japan)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Nippon nōgei kagakukaishi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TATEBE, W</au><au>MURAJI, M</au><au>FUJII, T</au><aucorp>Technische Univ. Berlin (Germany). Inst. fuer Oekologie. Fachgebiete Bodenkunde/Standortkunde und Bodenschutz</aucorp><aucorp>Osaka City Univ. (Japan)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The relationship between electropermeabilization and growth phase in a suspension of yeast cells, Saccharomyces cerevisiae</atitle><jtitle>Nippon nōgei kagakukaishi</jtitle><date>1996</date><risdate>1996</risdate><volume>70</volume><issue>5</issue><spage>563</spage><epage>568</epage><pages>563-568</pages><issn>0002-1407</issn><coden>NNKKAA</coden><abstract>We examined the relationship between growth phase and the initial field strength required for electropermeabilization of yeast cells in suspension. As the period of time for the culture was increased, the initial field strength necessary for electropermeabilization of the cells also increased and the slope of the graph of the number of perforations against field strength became smaller. These findings suggest a correlation between growth phase and membrane strength for electric pulses. In "decelerated growth phase", there may have been a greater overall increase in membrane strength than in the case of "exponential growth phase". In a further, "resealing" experiment, observations lead us to conclude that differences in the growth phase of Saccharomyces cerevisiae tended not to influence the rate of membrane resealing.</abstract><cop>Tokyo</cop><pub>Nippon Nōgei Kagakukai</pub><doi>10.1271/nogeikagaku1924.70.563</doi><tpages>6</tpages></addata></record> |
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| ispartof | Nippon nōgei kagakukaishi, 1996, Vol.70 (5), p.563-568 |
| issn | 0002-1407 |
| language | jpn |
| recordid | cdi_pascalfrancis_primary_3116312 |
| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | cells cellule celulas electroporacion electroporation growth period periode de croissance periodo de crecimiento saccharomyces cerevisiae |
| title | The relationship between electropermeabilization and growth phase in a suspension of yeast cells, Saccharomyces cerevisiae |
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