PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples

PCR procedures based on 16S rRNA gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. Fusobacte...

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Veröffentlicht in:	Applied and Environmental Microbiology 1996-04, Vol.62 (4), p.1242-1247
Hauptverfasser:	Wang, R.F. (Food and Drug Administration, Jefferson, AR.), Cao, W.W, Cerniglia, C.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Adult AMPLIFICATION CHAINE POLYMERASE ANAEROBIOSE ANAEROBIOSIS ANIMAL DE LABORATOIRE ANIMALES DE LABORATORIO Animals ARN RIBOSOMAL ARN RIBOSOMIAL Bacteria Bacteria, Anaerobic - genetics Bacteria, Anaerobic - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Bacteroides thetaiotaomicron Bacteroides vulgatus Base Sequence Biological and medical sciences Cats Cellular biology CHAT CHIEN Clostridium clostridiiforme Digestive system DNA Primers - genetics DNA, Bacterial - genetics Dogs Eubacterium limosum Evaluation Studies as Topic FECES Feces - microbiology FLORA MICROBIANA FLORE MICROBIENNE Fundamental and applied biological sciences. Psychology GATO GENE GENERO HUMANO GENES GENRE HUMAIN HECES Humans Infant INTESTIN INTESTINOS MAMIFEROS MAMMIFERE Mice Microbiology MICROORGANISME MICROORGANISMOS Molecular Sequence Data PERRO Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Rabbits Rats REACCION DE CADENAS DE POLIMERASA Ribonucleic acid RIBOSOMAS RIBOSOME RNA RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Sensitivity and Specificity
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