Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas
Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis from Brassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus...
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| Veröffentlicht in: | Planta 1996, Vol.198 (3), p.433-439 |
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| creator | Zhao, J.-P Simmonds, D.H Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology) |
| description | Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis from Brassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction in B. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development. |
| doi_str_mv | 10.1007/BF00620060 |
| format | Article |
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Topas</title><source>Jstor Complete Legacy</source><source>Springer Nature - Complete Springer Journals</source><creator>Zhao, J.-P ; Simmonds, D.H ; Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</creator><creatorcontrib>Zhao, J.-P ; Simmonds, D.H ; Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</creatorcontrib><description>Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis from Brassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction in B. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF00620060</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Anther and ovule culture, utilization for haploid production ; Biological and medical sciences ; Biotechnology ; BRASSICA NAPUS ; CALOR ; Cell walls ; CELLS ; CELLULE ; CELULAS ; CHALEUR ; CHROMOSOME ; CHROMOSOMES ; COLCHICINA ; COLCHICINE ; CROMOSOMAS ; CULTIVO DE EMBRIONES ; CULTURE D'EMBRYON ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; EMBRYO CULTURE ; Embryogenese ; Embryogenesis ; EMBRYONIC DEVELOPMENT ; Embryos ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; HAPLOIDIA ; HAPLOIDIE ; HAPLOIDY ; HEAT ; Heat treatment ; METHODE ; METHODS ; Methods. Procedures. Technologies ; METODOS ; Microspores ; Microsporocytes ; Microtubules ; Mikrospore ; Mikrotubulus ; Pflanzenbau ; Pflanzenentwicklung ; Pflanzenphysiologie ; Pflanzenzuechtung ; Plant cells and fungal cells ; Plant physiology and development ; POLEN ; POLLEN ; Population ; PROTEINAS ; PROTEINE ; PROTEINS ; Shock heating ; TEMPERATURA ; TEMPERATURE ; Tissue cultures, protoplasts ; Vakuole ; Washing ; Zellentwicklung</subject><ispartof>Planta, 1996, Vol.198 (3), p.433-439</ispartof><rights>Springer-Verlag 1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23384327$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23384327$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2981812$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, J.-P</creatorcontrib><creatorcontrib>Simmonds, D.H</creatorcontrib><creatorcontrib>Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</creatorcontrib><title>Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas</title><title>Planta</title><description>Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis from Brassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction in B. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.</description><subject>Anther and ovule culture, utilization for haploid production</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>BRASSICA NAPUS</subject><subject>CALOR</subject><subject>Cell walls</subject><subject>CELLS</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CHALEUR</subject><subject>CHROMOSOME</subject><subject>CHROMOSOMES</subject><subject>COLCHICINA</subject><subject>COLCHICINE</subject><subject>CROMOSOMAS</subject><subject>CULTIVO DE EMBRIONES</subject><subject>CULTURE D'EMBRYON</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>EMBRYO CULTURE</subject><subject>Embryogenese</subject><subject>Embryogenesis</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>Embryos</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HAPLOIDIA</subject><subject>HAPLOIDIE</subject><subject>HAPLOIDY</subject><subject>HEAT</subject><subject>Heat treatment</subject><subject>METHODE</subject><subject>METHODS</subject><subject>Methods. Procedures. Technologies</subject><subject>METODOS</subject><subject>Microspores</subject><subject>Microsporocytes</subject><subject>Microtubules</subject><subject>Mikrospore</subject><subject>Mikrotubulus</subject><subject>Pflanzenbau</subject><subject>Pflanzenentwicklung</subject><subject>Pflanzenphysiologie</subject><subject>Pflanzenzuechtung</subject><subject>Plant cells and fungal cells</subject><subject>Plant physiology and development</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Population</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Shock heating</subject><subject>TEMPERATURA</subject><subject>TEMPERATURE</subject><subject>Tissue cultures, protoplasts</subject><subject>Vakuole</subject><subject>Washing</subject><subject>Zellentwicklung</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpFkM9LwzAUx4MoOKcXj4KQg9fWl6RNk6Ob2xwMvMzzSJN0zdiaknTK_nszJnh4vB_fD98HX4QeCeQEoHqdzAE4TQVXaEQKRjMKhbhGI4A0g2TlLbqLcQeQxKoaoXbZmaMenO-wb7A91OHkt7az0UX844YWa7_XrdOus9h1cbDKnMHWqiHt-OB08LH3wcbzeRJUjE4r3Kn-GPEqx_o7x2vfq3iPbhq1j_bhr4_R13y2nn5kq8_Fcvq2yhpK2ZAJqCtQJW8KUwjgphSyFkpIrinlBqwpSa0ItyWTRVEZw7mgRBDGrdGSyIaN0cvFN_3Uat8E1WkXN31wBxVOGypFwmnCni_YLg4-_MuMiZRalfSni94ov1HbkCzeZ5IvZEqZ_QKKsGnk</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Zhao, J.-P</creator><creator>Simmonds, D.H</creator><creator>Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>1996</creationdate><title>Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas</title><author>Zhao, J.-P ; Simmonds, D.H ; Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f223t-80b70a56f4d4806d589b8a896c226d0ed51ba16e539447dd668218136edc919f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Anther and ovule culture, utilization for haploid production</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>BRASSICA NAPUS</topic><topic>CALOR</topic><topic>Cell walls</topic><topic>CELLS</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CHALEUR</topic><topic>CHROMOSOME</topic><topic>CHROMOSOMES</topic><topic>COLCHICINA</topic><topic>COLCHICINE</topic><topic>CROMOSOMAS</topic><topic>CULTIVO DE EMBRIONES</topic><topic>CULTURE D'EMBRYON</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>EMBRYO CULTURE</topic><topic>Embryogenese</topic><topic>Embryogenesis</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>Embryos</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HAPLOIDIA</topic><topic>HAPLOIDIE</topic><topic>HAPLOIDY</topic><topic>HEAT</topic><topic>Heat treatment</topic><topic>METHODE</topic><topic>METHODS</topic><topic>Methods. Procedures. Technologies</topic><topic>METODOS</topic><topic>Microspores</topic><topic>Microsporocytes</topic><topic>Microtubules</topic><topic>Mikrospore</topic><topic>Mikrotubulus</topic><topic>Pflanzenbau</topic><topic>Pflanzenentwicklung</topic><topic>Pflanzenphysiologie</topic><topic>Pflanzenzuechtung</topic><topic>Plant cells and fungal cells</topic><topic>Plant physiology and development</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>Population</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Shock heating</topic><topic>TEMPERATURA</topic><topic>TEMPERATURE</topic><topic>Tissue cultures, protoplasts</topic><topic>Vakuole</topic><topic>Washing</topic><topic>Zellentwicklung</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, J.-P</creatorcontrib><creatorcontrib>Simmonds, D.H</creatorcontrib><creatorcontrib>Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, J.-P</au><au>Simmonds, D.H</au><au>Newcomb, W. (Queen's Univ., Kingston, Ontario (Canada). Dept. of Biology)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas</atitle><jtitle>Planta</jtitle><date>1996</date><risdate>1996</risdate><volume>198</volume><issue>3</issue><spage>433</spage><epage>439</epage><pages>433-439</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis from Brassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction in B. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><doi>10.1007/BF00620060</doi><tpages>7</tpages></addata></record> |
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| ispartof | Planta, 1996, Vol.198 (3), p.433-439 |
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| source | Jstor Complete Legacy; Springer Nature - Complete Springer Journals |
| subjects | Anther and ovule culture, utilization for haploid production Biological and medical sciences Biotechnology BRASSICA NAPUS CALOR Cell walls CELLS CELLULE CELULAS CHALEUR CHROMOSOME CHROMOSOMES COLCHICINA COLCHICINE CROMOSOMAS CULTIVO DE EMBRIONES CULTURE D'EMBRYON DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE EMBRYO CULTURE Embryogenese Embryogenesis EMBRYONIC DEVELOPMENT Embryos Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology HAPLOIDIA HAPLOIDIE HAPLOIDY HEAT Heat treatment METHODE METHODS Methods. Procedures. Technologies METODOS Microspores Microsporocytes Microtubules Mikrospore Mikrotubulus Pflanzenbau Pflanzenentwicklung Pflanzenphysiologie Pflanzenzuechtung Plant cells and fungal cells Plant physiology and development POLEN POLLEN Population PROTEINAS PROTEINE PROTEINS Shock heating TEMPERATURA TEMPERATURE Tissue cultures, protoplasts Vakuole Washing Zellentwicklung |
| title | Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas |
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