Class I beta-1,3-glucanases in the endosperm of tobacco during germination
Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum L. cv Havana 425 seeds. Treatment with 10(-5) M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and r...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1995-11, Vol.109 (3), p.751-759 |
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| creator | Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.) Frundt, C Vogeli-Lange, R Meins, F. Jr |
| description | Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum L. cv Havana 425 seeds. Treatment with 10(-5) M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and resulted in the formation of a novel structure consisting of the enlarging radicle with a sheath of greatly elongated endosperm tissue. Therefore, ABA appears to act primarily by delaying endosperm rupture and radicle emergence. Measurements of beta-1,3-glucanase activity, antigen content, and mRNA accumulation together with reporter gene experiments showed that induction of class I beta-1,3-glucanase genes begins just prior to the onset of endosperm rupture but after the completion of seed-coat rupture. This induction was localized exclusively in the micropylar region of the endosperm, where the radicle will penetrate. ABA treatment markedly inhibited the rate of beta-1,3-glucanase accumulation but did not delay the onset of induction. Independent of the ABA concentration used, onset of endosperm rupture was correlated with the same beta-1,3-glucanase content/seed. These results suggest that ABA-sensitive class I beta-1,3-glucanases promote radicle penetration of the endosperm, which is a key limiting step in tobacco seed germination |
| doi_str_mv | 10.1104/pp.109.3.751 |
| format | Article |
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(Friedrich-Miescher Institute, Basel, Switzerland.) ; Frundt, C ; Vogeli-Lange, R ; Meins, F. Jr</creator><creatorcontrib>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.) ; Frundt, C ; Vogeli-Lange, R ; Meins, F. Jr</creatorcontrib><description>Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum L. cv Havana 425 seeds. Treatment with 10(-5) M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and resulted in the formation of a novel structure consisting of the enlarging radicle with a sheath of greatly elongated endosperm tissue. Therefore, ABA appears to act primarily by delaying endosperm rupture and radicle emergence. Measurements of beta-1,3-glucanase activity, antigen content, and mRNA accumulation together with reporter gene experiments showed that induction of class I beta-1,3-glucanase genes begins just prior to the onset of endosperm rupture but after the completion of seed-coat rupture. This induction was localized exclusively in the micropylar region of the endosperm, where the radicle will penetrate. ABA treatment markedly inhibited the rate of beta-1,3-glucanase accumulation but did not delay the onset of induction. Independent of the ABA concentration used, onset of endosperm rupture was correlated with the same beta-1,3-glucanase content/seed. These results suggest that ABA-sensitive class I beta-1,3-glucanases promote radicle penetration of the endosperm, which is a key limiting step in tobacco seed germination</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.109.3.751</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ABA ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ANATOMIA DE LA PLANTA ; ANATOMIE VEGETALE ; ARN MENSAJERO ; ARN MESSAGER ; Biological and medical sciences ; CHIMIE ; Development and Growth Regulation ; Embryos ; EMERGENCIA ; Endosperm ; ENDOSPERMA ; ENDOSPERME ; Enzymes ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; Gene expression regulation ; GENES ; GERMINACION ; GERMINATION ; Germination and dormancy ; GLICOSIDASAS ; GLYCOSIDASE ; GRAINE ; LEVEE ; Messenger RNA ; NICOTIANA TABACUM ; Plant physiology and development ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; PROTEINAS ; PROTEINE ; QUIMICA ; RADICULA ; RADICULE ; SECUENCIA NUCLEOTIDICA ; Seed germination ; SEMILLA ; SEQUENCE NUCLEOTIDIQUE ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; TESTA ; Traumatic ruptures</subject><ispartof>Plant physiology (Bethesda), 1995-11, Vol.109 (3), p.751-759</ispartof><rights>Copyright 1995 American Society of Plant Physiologists</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4276864$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4276864$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2914807$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.)</creatorcontrib><creatorcontrib>Frundt, C</creatorcontrib><creatorcontrib>Vogeli-Lange, R</creatorcontrib><creatorcontrib>Meins, F. Jr</creatorcontrib><title>Class I beta-1,3-glucanases in the endosperm of tobacco during germination</title><title>Plant physiology (Bethesda)</title><description>Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum L. cv Havana 425 seeds. Treatment with 10(-5) M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and resulted in the formation of a novel structure consisting of the enlarging radicle with a sheath of greatly elongated endosperm tissue. Therefore, ABA appears to act primarily by delaying endosperm rupture and radicle emergence. Measurements of beta-1,3-glucanase activity, antigen content, and mRNA accumulation together with reporter gene experiments showed that induction of class I beta-1,3-glucanase genes begins just prior to the onset of endosperm rupture but after the completion of seed-coat rupture. This induction was localized exclusively in the micropylar region of the endosperm, where the radicle will penetrate. ABA treatment markedly inhibited the rate of beta-1,3-glucanase accumulation but did not delay the onset of induction. Independent of the ABA concentration used, onset of endosperm rupture was correlated with the same beta-1,3-glucanase content/seed. These results suggest that ABA-sensitive class I beta-1,3-glucanases promote radicle penetration of the endosperm, which is a key limiting step in tobacco seed germination</description><subject>ABA</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ANATOMIA DE LA PLANTA</subject><subject>ANATOMIE VEGETALE</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Biological and medical sciences</subject><subject>CHIMIE</subject><subject>Development and Growth Regulation</subject><subject>Embryos</subject><subject>EMERGENCIA</subject><subject>Endosperm</subject><subject>ENDOSPERMA</subject><subject>ENDOSPERME</subject><subject>Enzymes</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Gene expression regulation</subject><subject>GENES</subject><subject>GERMINACION</subject><subject>GERMINATION</subject><subject>Germination and dormancy</subject><subject>GLICOSIDASAS</subject><subject>GLYCOSIDASE</subject><subject>GRAINE</subject><subject>LEVEE</subject><subject>Messenger RNA</subject><subject>NICOTIANA TABACUM</subject><subject>Plant physiology and development</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>QUIMICA</subject><subject>RADICULA</subject><subject>RADICULE</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Seed germination</subject><subject>SEMILLA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>TESTA</subject><subject>Traumatic ruptures</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNo9jb1PwzAUxC0EEqWwMSEGD4wk-DOxR1TxUVSJATpXz3YcUqV2ZIeB_x5LRUz3dL-7ewhdU1JTSsTDNNWU6JrXraQnaEElZxWTQp2iBSHlJkrpc3SR854QQjkVC_S2GiFnvMamm6Gi97zqx28LAXKX8RDw_NXhLriYpy4dcPR4jgasjdh9pyH0uC_2EGAeYrhEZx7G3F396RJtn58-V6_V5v1lvXrcVJ4xNleNNJJyoYRomBNaSu2UEbSIBKMFax0BBl570hAmbcsbYbgzptRMq5zlS3R33J0gWxh9gmCHvJvScID0s2OaCkXaErs9xvZ5jukflweNakTBN0fsIe6gT2Vh-6FbylrO-C9O0F8A</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.)</creator><creator>Frundt, C</creator><creator>Vogeli-Lange, R</creator><creator>Meins, F. Jr</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>19951101</creationdate><title>Class I beta-1,3-glucanases in the endosperm of tobacco during germination</title><author>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.) ; Frundt, C ; Vogeli-Lange, R ; Meins, F. Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f222t-65b513484462d49559d8b4159d5ab9427d0a2af9f06025c7364b3dbb5b5b78dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ABA</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ANATOMIA DE LA PLANTA</topic><topic>ANATOMIE VEGETALE</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Biological and medical sciences</topic><topic>CHIMIE</topic><topic>Development and Growth Regulation</topic><topic>Embryos</topic><topic>EMERGENCIA</topic><topic>Endosperm</topic><topic>ENDOSPERMA</topic><topic>ENDOSPERME</topic><topic>Enzymes</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Gene expression regulation</topic><topic>GENES</topic><topic>GERMINACION</topic><topic>GERMINATION</topic><topic>Germination and dormancy</topic><topic>GLICOSIDASAS</topic><topic>GLYCOSIDASE</topic><topic>GRAINE</topic><topic>LEVEE</topic><topic>Messenger RNA</topic><topic>NICOTIANA TABACUM</topic><topic>Plant physiology and development</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>QUIMICA</topic><topic>RADICULA</topic><topic>RADICULE</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Seed germination</topic><topic>SEMILLA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>TESTA</topic><topic>Traumatic ruptures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.)</creatorcontrib><creatorcontrib>Frundt, C</creatorcontrib><creatorcontrib>Vogeli-Lange, R</creatorcontrib><creatorcontrib>Meins, F. Jr</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leubner-Metzger, G. (Friedrich-Miescher Institute, Basel, Switzerland.)</au><au>Frundt, C</au><au>Vogeli-Lange, R</au><au>Meins, F. Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Class I beta-1,3-glucanases in the endosperm of tobacco during germination</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>1995-11-01</date><risdate>1995</risdate><volume>109</volume><issue>3</issue><spage>751</spage><epage>759</epage><pages>751-759</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum L. cv Havana 425 seeds. Treatment with 10(-5) M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and resulted in the formation of a novel structure consisting of the enlarging radicle with a sheath of greatly elongated endosperm tissue. Therefore, ABA appears to act primarily by delaying endosperm rupture and radicle emergence. Measurements of beta-1,3-glucanase activity, antigen content, and mRNA accumulation together with reporter gene experiments showed that induction of class I beta-1,3-glucanase genes begins just prior to the onset of endosperm rupture but after the completion of seed-coat rupture. This induction was localized exclusively in the micropylar region of the endosperm, where the radicle will penetrate. ABA treatment markedly inhibited the rate of beta-1,3-glucanase accumulation but did not delay the onset of induction. Independent of the ABA concentration used, onset of endosperm rupture was correlated with the same beta-1,3-glucanase content/seed. These results suggest that ABA-sensitive class I beta-1,3-glucanases promote radicle penetration of the endosperm, which is a key limiting step in tobacco seed germination</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><doi>10.1104/pp.109.3.751</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1995-11, Vol.109 (3), p.751-759 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_2914807 |
| source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ABA ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ANATOMIA DE LA PLANTA ANATOMIE VEGETALE ARN MENSAJERO ARN MESSAGER Biological and medical sciences CHIMIE Development and Growth Regulation Embryos EMERGENCIA Endosperm ENDOSPERMA ENDOSPERME Enzymes EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE Gene expression regulation GENES GERMINACION GERMINATION Germination and dormancy GLICOSIDASAS GLYCOSIDASE GRAINE LEVEE Messenger RNA NICOTIANA TABACUM Plant physiology and development PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants PROTEINAS PROTEINE QUIMICA RADICULA RADICULE SECUENCIA NUCLEOTIDICA Seed germination SEMILLA SEQUENCE NUCLEOTIDIQUE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS TESTA Traumatic ruptures |
| title | Class I beta-1,3-glucanases in the endosperm of tobacco during germination |
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