Improvement of the maturation and germination of horse chestnut somatic embryos
Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 1997, Vol.48 (1), p.23-29 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Capuana, M Debergh, P.C |
| description | Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-seated Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 micromolar) alone or plus PEG (50 g l-1). |
| doi_str_mv | 10.1023/A:1005890826431 |
| format | Article |
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Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-seated Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 micromolar) alone or plus PEG (50 g l-1).</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1023/A:1005890826431</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>2,4-D ; abscisic acid ; Aesculus hippocastanum ; benzyladenine ; Biological and medical sciences ; Biotechnology ; charcoal ; culture media ; desiccation ; dose response ; embryo (plant) ; embryo culture ; embryogenesis ; Establishment of new cell lines, improvement of cultural methods, mass culture ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; germination ; growth ; in vitro culture ; indole butyric acid ; mannitol ; maturation ; methodology ; Methods. Procedures. Technologies ; micropropagation ; murashige and skoog medium ; Plant cells and fungal cells ; polyethylene glycol ; shoots ; stamens ; viability</subject><ispartof>Plant cell, tissue and organ culture, 1997, Vol.48 (1), p.23-29</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c252t-6a085fd552c6160ed20b63880e6982233686e99c2839e97e0affe48ffc34e0d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2743927$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Capuana, M</creatorcontrib><creatorcontrib>Debergh, P.C</creatorcontrib><title>Improvement of the maturation and germination of horse chestnut somatic embryos</title><title>Plant cell, tissue and organ culture</title><description>Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-seated Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 micromolar) alone or plus PEG (50 g l-1).</description><subject>2,4-D</subject><subject>abscisic acid</subject><subject>Aesculus hippocastanum</subject><subject>benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>charcoal</subject><subject>culture media</subject><subject>desiccation</subject><subject>dose response</subject><subject>embryo (plant)</subject><subject>embryo culture</subject><subject>embryogenesis</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>germination</subject><subject>growth</subject><subject>in vitro culture</subject><subject>indole butyric acid</subject><subject>mannitol</subject><subject>maturation</subject><subject>methodology</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>murashige and skoog medium</subject><subject>Plant cells and fungal cells</subject><subject>polyethylene glycol</subject><subject>shoots</subject><subject>stamens</subject><subject>viability</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNotzUtLAzEUBeAgCtbq2qVZuB29eSfuSvFRKHShXZc0c9OOdCYlSQX_vQN1dTjwcQ4h9wyeGHDxPHthAMo6sFxLwS7IhCkjGgVSXpIJMG0abZW5JjelfAOAFpJNyGrRH3P6wR6HSlOkdY-09_WUfe3SQP3Q0h3mvhvOfRT7lAvSsMdSh1OlJY28CxT7bf5N5ZZcRX8oePefU7J-e_2afzTL1ftiPls2gSteG-3BqtgqxYNmGrDlsNXCWkDtLOdCaKvRucCtcOgMgo8RpY0xCInQMjElj-fdoy_BH2L2Q-jK5pi73uffDTdSOG5G9nBm0aeN3-WRrD85MAHcmvGPiz8f81qt</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Capuana, M</creator><creator>Debergh, P.C</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>1997</creationdate><title>Improvement of the maturation and germination of horse chestnut somatic embryos</title><author>Capuana, M ; Debergh, P.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c252t-6a085fd552c6160ed20b63880e6982233686e99c2839e97e0affe48ffc34e0d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>2,4-D</topic><topic>abscisic acid</topic><topic>Aesculus hippocastanum</topic><topic>benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>charcoal</topic><topic>culture media</topic><topic>desiccation</topic><topic>dose response</topic><topic>embryo (plant)</topic><topic>embryo culture</topic><topic>embryogenesis</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass culture</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>germination</topic><topic>growth</topic><topic>in vitro culture</topic><topic>indole butyric acid</topic><topic>mannitol</topic><topic>maturation</topic><topic>methodology</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>murashige and skoog medium</topic><topic>Plant cells and fungal cells</topic><topic>polyethylene glycol</topic><topic>shoots</topic><topic>stamens</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capuana, M</creatorcontrib><creatorcontrib>Debergh, P.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capuana, M</au><au>Debergh, P.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of the maturation and germination of horse chestnut somatic embryos</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><date>1997</date><risdate>1997</risdate><volume>48</volume><issue>1</issue><spage>23</spage><epage>29</epage><pages>23-29</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-seated Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 micromolar) alone or plus PEG (50 g l-1).</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1023/A:1005890826431</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 1997, Vol.48 (1), p.23-29 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | 2,4-D abscisic acid Aesculus hippocastanum benzyladenine Biological and medical sciences Biotechnology charcoal culture media desiccation dose response embryo (plant) embryo culture embryogenesis Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology germination growth in vitro culture indole butyric acid mannitol maturation methodology Methods. Procedures. Technologies micropropagation murashige and skoog medium Plant cells and fungal cells polyethylene glycol shoots stamens viability |
| title | Improvement of the maturation and germination of horse chestnut somatic embryos |
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