Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece
A significant increase in the frequency of isolation of Salmonella enteritidis has been observed during recent years in Greece, parallelled by an increasing rate of resistance of this organism to antibiotics. A substantial proportion of ampicillin- and doxycycline-resistant isolates exhibited cross-...
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| Veröffentlicht in: | Journal of Clinical Microbiology 1997-06, Vol.35 (6), p.1316-1321 |
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| creator | Tassios, P.T. (University of Athens, Greece.) Markogiannakis, A Vatopoulos, A.C Katsanikou, E Velonakis, E.N Kourea-Kremastinou, J Legakis, N.J |
| description | A significant increase in the frequency of isolation of Salmonella enteritidis has been observed during recent years in Greece, parallelled by an increasing rate of resistance of this organism to antibiotics. A substantial proportion of ampicillin- and doxycycline-resistant isolates exhibited cross-resistance to drugs of other classes, such as sulfonamides and streptomycin. Isolates of human origin were overall less resistant than those of animal or food-feed origin. Indeed, strains associated with animal infections were characterized by the highest rates of resistance to several antibiotics. These phenotypic data were correlated with genotypic information concerning two distinct populations: isolates from all sources that were resistant only to ampicillin, the drug toward which resistance rates were highest, and a control group of sensitive isolates. Ampicillin resistance was due to a 34-MDa conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed two types, A and B, common to both ampicillin-resistant and -sensitive strains, with 80 to 90% of strains being of type A. However, a third type, C, was specific for the sensitive population, representing 17% of those strains. Therefore, although the majority of resistant isolates were genetically related to sensitive ones, there existed a susceptible clone which had not acquired any resistance traits |
| doi_str_mv | 10.1128/jcm.35.6.1316-1321.1997 |
| format | Article |
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(University of Athens, Greece.) ; Markogiannakis, A ; Vatopoulos, A.C ; Katsanikou, E ; Velonakis, E.N ; Kourea-Kremastinou, J ; Legakis, N.J</creator><creatorcontrib>Tassios, P.T. (University of Athens, Greece.) ; Markogiannakis, A ; Vatopoulos, A.C ; Katsanikou, E ; Velonakis, E.N ; Kourea-Kremastinou, J ; Legakis, N.J</creatorcontrib><description>A significant increase in the frequency of isolation of Salmonella enteritidis has been observed during recent years in Greece, parallelled by an increasing rate of resistance of this organism to antibiotics. A substantial proportion of ampicillin- and doxycycline-resistant isolates exhibited cross-resistance to drugs of other classes, such as sulfonamides and streptomycin. Isolates of human origin were overall less resistant than those of animal or food-feed origin. Indeed, strains associated with animal infections were characterized by the highest rates of resistance to several antibiotics. These phenotypic data were correlated with genotypic information concerning two distinct populations: isolates from all sources that were resistant only to ampicillin, the drug toward which resistance rates were highest, and a control group of sensitive isolates. Ampicillin resistance was due to a 34-MDa conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed two types, A and B, common to both ampicillin-resistant and -sensitive strains, with 80 to 90% of strains being of type A. However, a third type, C, was specific for the sensitive population, representing 17% of those strains. Therefore, although the majority of resistant isolates were genetically related to sensitive ones, there existed a susceptible clone which had not acquired any resistance traits</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.35.6.1316-1321.1997</identifier><identifier>PMID: 9163436</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Ampicillin - pharmacology ; Ampicillin Resistance - genetics ; Animals ; Antibacterial agents ; ANTIBIOTICOS ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; ANTIBIOTIQUE ; Bacteriology ; Biological and medical sciences ; Drug Resistance, Microbial - genetics ; EPIDEMIOLOGIA ; EPIDEMIOLOGIE ; Epidemiology ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; GRECE ; GRECIA ; Greece - epidemiology ; Humans ; Medical sciences ; Microbial Sensitivity Tests ; Microbiology ; Molecular Epidemiology ; Penicillins - pharmacology ; Pharmacology. Drug treatments ; R Factors - analysis ; Restriction Mapping ; SALMONELLA ENTERITIDIS ; Salmonella enteritidis - drug effects ; Salmonella enteritidis - genetics ; Salmonella Infections - epidemiology ; Salmonella Infections - microbiology</subject><ispartof>Journal of Clinical Microbiology, 1997-06, Vol.35 (6), p.1316-1321</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c636t-b81a01e649776fdf66234d0640bf053ade85a6d89d37ff1ad3f827d0aa249eab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229741/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229741/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,3175,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2689063$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9163436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tassios, P.T. (University of Athens, Greece.)</creatorcontrib><creatorcontrib>Markogiannakis, A</creatorcontrib><creatorcontrib>Vatopoulos, A.C</creatorcontrib><creatorcontrib>Katsanikou, E</creatorcontrib><creatorcontrib>Velonakis, E.N</creatorcontrib><creatorcontrib>Kourea-Kremastinou, J</creatorcontrib><creatorcontrib>Legakis, N.J</creatorcontrib><title>Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>A significant increase in the frequency of isolation of Salmonella enteritidis has been observed during recent years in Greece, parallelled by an increasing rate of resistance of this organism to antibiotics. A substantial proportion of ampicillin- and doxycycline-resistant isolates exhibited cross-resistance to drugs of other classes, such as sulfonamides and streptomycin. Isolates of human origin were overall less resistant than those of animal or food-feed origin. Indeed, strains associated with animal infections were characterized by the highest rates of resistance to several antibiotics. These phenotypic data were correlated with genotypic information concerning two distinct populations: isolates from all sources that were resistant only to ampicillin, the drug toward which resistance rates were highest, and a control group of sensitive isolates. Ampicillin resistance was due to a 34-MDa conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed two types, A and B, common to both ampicillin-resistant and -sensitive strains, with 80 to 90% of strains being of type A. However, a third type, C, was specific for the sensitive population, representing 17% of those strains. Therefore, although the majority of resistant isolates were genetically related to sensitive ones, there existed a susceptible clone which had not acquired any resistance traits</description><subject>Ampicillin - pharmacology</subject><subject>Ampicillin Resistance - genetics</subject><subject>Animals</subject><subject>Antibacterial agents</subject><subject>ANTIBIOTICOS</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>ANTIBIOTIQUE</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>EPIDEMIOLOGIA</subject><subject>EPIDEMIOLOGIE</subject><subject>Epidemiology</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GRECE</subject><subject>GRECIA</subject><subject>Greece - epidemiology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Molecular Epidemiology</subject><subject>Penicillins - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>R Factors - analysis</subject><subject>Restriction Mapping</subject><subject>SALMONELLA ENTERITIDIS</subject><subject>Salmonella enteritidis - drug effects</subject><subject>Salmonella enteritidis - genetics</subject><subject>Salmonella Infections - epidemiology</subject><subject>Salmonella Infections - microbiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1u1DAUhS0EKkPhBZAQQarYJdhx4sSLLlBVClIRi1KJnXVjX2c8SuLBTkDz9jia0Yiu7uJ85_4dQt4zWjBWtp92eix4XYiCcSZyxktWMCmbZ2TDqGxzIeiv52RDqaxzxnjzkryKcUcpq6q6viAXkglecbEhu-9-QL0MEDLcO4Oj84PvD5m3GUyz65yfnc4CRhdnmDSuwgMMo59wGCDDacbgZmdczMwS3NRnkDX5AVO_fVK8ydyU3QVEja_JCwtDxDenekkev9z-vPma3_-4-3bz-T7Xgos571oGlKGoZNMIa6wQJa8MFRXtLK05GGxrEKaVhjfWMjDctmVjKEBZSYSOX5LrY9_90o1odNoxwKD2wY0QDsqDU0-VyW1V7_-ospRNxZL_48kf_O8F46xGF_V67oR-iYoJmn7ciAQ2R1AHH2NAe57BqFpTUiklxWsl1JqSWlNSa0rJ-e7_Fc--UyxJvzrpEDUMNqTXu3jGStFKKnjCPhyxreu3f11ABXF8OjQxb4-MBa-gD6nN40NaQdJ0wT_5I7H_</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Tassios, P.T. (University of Athens, Greece.)</creator><creator>Markogiannakis, A</creator><creator>Vatopoulos, A.C</creator><creator>Katsanikou, E</creator><creator>Velonakis, E.N</creator><creator>Kourea-Kremastinou, J</creator><creator>Legakis, N.J</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>19970601</creationdate><title>Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece</title><author>Tassios, P.T. (University of Athens, Greece.) ; Markogiannakis, A ; Vatopoulos, A.C ; Katsanikou, E ; Velonakis, E.N ; Kourea-Kremastinou, J ; Legakis, N.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c636t-b81a01e649776fdf66234d0640bf053ade85a6d89d37ff1ad3f827d0aa249eab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Ampicillin - pharmacology</topic><topic>Ampicillin Resistance - genetics</topic><topic>Animals</topic><topic>Antibacterial agents</topic><topic>ANTIBIOTICOS</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>ANTIBIOTIQUE</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>EPIDEMIOLOGIA</topic><topic>EPIDEMIOLOGIE</topic><topic>Epidemiology</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GRECE</topic><topic>GRECIA</topic><topic>Greece - epidemiology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Molecular Epidemiology</topic><topic>Penicillins - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>R Factors - analysis</topic><topic>Restriction Mapping</topic><topic>SALMONELLA ENTERITIDIS</topic><topic>Salmonella enteritidis - drug effects</topic><topic>Salmonella enteritidis - genetics</topic><topic>Salmonella Infections - epidemiology</topic><topic>Salmonella Infections - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tassios, P.T. (University of Athens, Greece.)</creatorcontrib><creatorcontrib>Markogiannakis, A</creatorcontrib><creatorcontrib>Vatopoulos, A.C</creatorcontrib><creatorcontrib>Katsanikou, E</creatorcontrib><creatorcontrib>Velonakis, E.N</creatorcontrib><creatorcontrib>Kourea-Kremastinou, J</creatorcontrib><creatorcontrib>Legakis, N.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tassios, P.T. (University of Athens, Greece.)</au><au>Markogiannakis, A</au><au>Vatopoulos, A.C</au><au>Katsanikou, E</au><au>Velonakis, E.N</au><au>Kourea-Kremastinou, J</au><au>Legakis, N.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>35</volume><issue>6</issue><spage>1316</spage><epage>1321</epage><pages>1316-1321</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>A significant increase in the frequency of isolation of Salmonella enteritidis has been observed during recent years in Greece, parallelled by an increasing rate of resistance of this organism to antibiotics. A substantial proportion of ampicillin- and doxycycline-resistant isolates exhibited cross-resistance to drugs of other classes, such as sulfonamides and streptomycin. Isolates of human origin were overall less resistant than those of animal or food-feed origin. Indeed, strains associated with animal infections were characterized by the highest rates of resistance to several antibiotics. These phenotypic data were correlated with genotypic information concerning two distinct populations: isolates from all sources that were resistant only to ampicillin, the drug toward which resistance rates were highest, and a control group of sensitive isolates. Ampicillin resistance was due to a 34-MDa conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed two types, A and B, common to both ampicillin-resistant and -sensitive strains, with 80 to 90% of strains being of type A. However, a third type, C, was specific for the sensitive population, representing 17% of those strains. Therefore, although the majority of resistant isolates were genetically related to sensitive ones, there existed a susceptible clone which had not acquired any resistance traits</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9163436</pmid><doi>10.1128/jcm.35.6.1316-1321.1997</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Ampicillin - pharmacology Ampicillin Resistance - genetics Animals Antibacterial agents ANTIBIOTICOS Antibiotics. Antiinfectious agents. Antiparasitic agents ANTIBIOTIQUE Bacteriology Biological and medical sciences Drug Resistance, Microbial - genetics EPIDEMIOLOGIA EPIDEMIOLOGIE Epidemiology Food Microbiology Fundamental and applied biological sciences. Psychology GRECE GRECIA Greece - epidemiology Humans Medical sciences Microbial Sensitivity Tests Microbiology Molecular Epidemiology Penicillins - pharmacology Pharmacology. Drug treatments R Factors - analysis Restriction Mapping SALMONELLA ENTERITIDIS Salmonella enteritidis - drug effects Salmonella enteritidis - genetics Salmonella Infections - epidemiology Salmonella Infections - microbiology |
| title | Molecular epidemiology of antibiotic resistance of Salmonella enteritidis during a 7-year period in Greece |
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