Practical application of immunofluorescence for the detection of bacterial contaminants during vinification
This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with...
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Veröffentlicht in: | Journal international des sciences de la vigne et du vin 1997-03, Vol.31 (1), p.11-22 |
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creator | BOUIX, M BUSSON, C CHARPENTIER, M LEVEAU, J. Y DUTEURTRE, B |
description | This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with Leuconostoc, Pediococcus and Lactobacillus concentrations as small as 102 cells/ml. Implementation of this technique will assist winemakers in controlling malolactic fermentations and in preventing lactic acid bacterial spoilage. |
doi_str_mv | 10.20870/oeno-one.1997.31.1.1089 |
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Y ; DUTEURTRE, B</creator><creatorcontrib>BOUIX, M ; BUSSON, C ; CHARPENTIER, M ; LEVEAU, J. Y ; DUTEURTRE, B</creatorcontrib><description>This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with Leuconostoc, Pediococcus and Lactobacillus concentrations as small as 102 cells/ml. Implementation of this technique will assist winemakers in controlling malolactic fermentations and in preventing lactic acid bacterial spoilage.</description><identifier>ISSN: 1151-0285</identifier><identifier>ISSN: 2494-1271</identifier><identifier>EISSN: 2494-1271</identifier><identifier>DOI: 10.20870/oeno-one.1997.31.1.1089</identifier><language>eng</language><publisher>Bordeaux: Vigne et vins</publisher><subject>bacterial contamination ; Biological and medical sciences ; detection ; Fermented food industries ; fluorescence ; fluorescent antibody technique ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; immunofluorescence ; inoculum ; lactic acid ; lactic acid bacteria ; Lactobacillus ; Leuconostoc ; Methods of analysis, processing and quality control, regulation, standards ; must ; Pediococcus ; spoilage ; wine ; winemaking ; Wines and vinegars</subject><ispartof>Journal international des sciences de la vigne et du vin, 1997-03, Vol.31 (1), p.11-22</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2624597$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>BOUIX, M</creatorcontrib><creatorcontrib>BUSSON, C</creatorcontrib><creatorcontrib>CHARPENTIER, M</creatorcontrib><creatorcontrib>LEVEAU, J. Y</creatorcontrib><creatorcontrib>DUTEURTRE, B</creatorcontrib><title>Practical application of immunofluorescence for the detection of bacterial contaminants during vinification</title><title>Journal international des sciences de la vigne et du vin</title><description>This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with Leuconostoc, Pediococcus and Lactobacillus concentrations as small as 102 cells/ml. Implementation of this technique will assist winemakers in controlling malolactic fermentations and in preventing lactic acid bacterial spoilage.</description><subject>bacterial contamination</subject><subject>Biological and medical sciences</subject><subject>detection</subject><subject>Fermented food industries</subject><subject>fluorescence</subject><subject>fluorescent antibody technique</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>immunofluorescence</subject><subject>inoculum</subject><subject>lactic acid</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus</subject><subject>Leuconostoc</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>must</subject><subject>Pediococcus</subject><subject>spoilage</subject><subject>wine</subject><subject>winemaking</subject><subject>Wines and vinegars</subject><issn>1151-0285</issn><issn>2494-1271</issn><issn>2494-1271</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNo9kUuPFSEQhTtGE2_G-Q8sXLjpK8WjgaWZ-JhkEl3omtBQjIzdcIVuE_-9zNzrpBaQyjmnUvUNAwF6ZFQr-r5gLmPJeARj1JHDsRfV5sVwYMKIEZiCl8MBQMJImZavh-vWHiilAEzCRA_Dr2_V-S15txB3Oi39s6WSSYkkreueS1z2UrF5zB5JLJVsP5EE3ND_183djzX1AF_y5taUXd4aCXtN-Z78STnFS-qb4VV0S8Pry3s1_Pj08fvNl_Hu6-fbmw93o2dGmjEo7YOB4GUUTGk2y0lG5TBoPSs2M0Ol9EJOiAKomyl44yfGJZ9C4EEafjXcnnNDcQ_2VNPq6l9bXLJPjVLvrat95wUtRB6DMW4GLYQSXFMWlfHMBw0KQ-xZ785Zp1p-79g2u6Z-jWVxGcveLJv4JDQ1GrpUn6W-ltYqxufRQO0TLvuIy3Zc9hGX5WB7dVzd-vYyxbWOIlaXfWrPfjYxIY3i_wBg8JmK</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>BOUIX, M</creator><creator>BUSSON, C</creator><creator>CHARPENTIER, M</creator><creator>LEVEAU, J. Y</creator><creator>DUTEURTRE, B</creator><general>Vigne et vins</general><general>International Viticulture and Enology Society</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>DOA</scope></search><sort><creationdate>19970301</creationdate><title>Practical application of immunofluorescence for the detection of bacterial contaminants during vinification</title><author>BOUIX, M ; BUSSON, C ; CHARPENTIER, M ; LEVEAU, J. Y ; DUTEURTRE, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2959-d78cd91dc5f42782b565f7aed88b72b29055c456ee410ab01c9c623536dd3d593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>bacterial contamination</topic><topic>Biological and medical sciences</topic><topic>detection</topic><topic>Fermented food industries</topic><topic>fluorescence</topic><topic>fluorescent antibody technique</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>immunofluorescence</topic><topic>inoculum</topic><topic>lactic acid</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus</topic><topic>Leuconostoc</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>must</topic><topic>Pediococcus</topic><topic>spoilage</topic><topic>wine</topic><topic>winemaking</topic><topic>Wines and vinegars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BOUIX, M</creatorcontrib><creatorcontrib>BUSSON, C</creatorcontrib><creatorcontrib>CHARPENTIER, M</creatorcontrib><creatorcontrib>LEVEAU, J. 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Y</au><au>DUTEURTRE, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Practical application of immunofluorescence for the detection of bacterial contaminants during vinification</atitle><jtitle>Journal international des sciences de la vigne et du vin</jtitle><date>1997-03-01</date><risdate>1997</risdate><volume>31</volume><issue>1</issue><spage>11</spage><epage>22</epage><pages>11-22</pages><issn>1151-0285</issn><issn>2494-1271</issn><eissn>2494-1271</eissn><abstract>This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with Leuconostoc, Pediococcus and Lactobacillus concentrations as small as 102 cells/ml. Implementation of this technique will assist winemakers in controlling malolactic fermentations and in preventing lactic acid bacterial spoilage.</abstract><cop>Bordeaux</cop><pub>Vigne et vins</pub><doi>10.20870/oeno-one.1997.31.1.1089</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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source | Alma/SFX Local Collection |
subjects | bacterial contamination Biological and medical sciences detection Fermented food industries fluorescence fluorescent antibody technique Food industries Fundamental and applied biological sciences. Psychology General aspects immunofluorescence inoculum lactic acid lactic acid bacteria Lactobacillus Leuconostoc Methods of analysis, processing and quality control, regulation, standards must Pediococcus spoilage wine winemaking Wines and vinegars |
title | Practical application of immunofluorescence for the detection of bacterial contaminants during vinification |
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