Natural senescence of pea leaves. An activated oxygen-mediated function for peroxisomes
We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activiti...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1997-02, Vol.113 (2), p.411-418 |
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| description | We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activities of the O2.- -producing and H2O2-producing enzymes were enhanced by natural senescence. Senescence also produced an increase in the generation of active oxygen species (O2.- and H2O2) in leaf peroxisomes and in the activities of two glyoxylate-cycle marker enzymes. A new fraction of peroxisomes was detected at an advanced stage of dark-induced senescence. Electron microscopy revealed that this new peroxisomal fraction varied in size and electron density. During senescence, the constitutive Mn-superoxide dismutase (SOD) activity of peroxisomes increased and two new CuZn-SODs were induced, one of which cross-reacted with an antibody against glyoxysomal CuZnSOD. This fact and the presence of glyoxylate-cycle enzymes support the idea that foliar senescence is associated with the transition of peroxisomes into glyoxysomes. Our results indicate that natural senescence causes the same changes in peroxisome-activated oxygen metabolism as dark-induced senescence, and reinforce the hypothesis of an effective role of peroxisomes and their activated oxygen metabolism in this stage of the life cycle. |
| doi_str_mv | 10.1104/pp.113.2.411 |
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An activated oxygen-mediated function for peroxisomes</title><source>JSTOR Archive Collection A-Z Listing</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>PASTORI, G. M ; DEL RIO, L. A</creator><creatorcontrib>PASTORI, G. M ; DEL RIO, L. A ; Estacion Experimental del Zaidin, Granada, Spain ; Eidgenoessische Technische Hochschule Zuerich (Switzerland). Professur Holzwissenschaften</creatorcontrib><description>We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activities of the O2.- -producing and H2O2-producing enzymes were enhanced by natural senescence. Senescence also produced an increase in the generation of active oxygen species (O2.- and H2O2) in leaf peroxisomes and in the activities of two glyoxylate-cycle marker enzymes. A new fraction of peroxisomes was detected at an advanced stage of dark-induced senescence. Electron microscopy revealed that this new peroxisomal fraction varied in size and electron density. During senescence, the constitutive Mn-superoxide dismutase (SOD) activity of peroxisomes increased and two new CuZn-SODs were induced, one of which cross-reacted with an antibody against glyoxysomal CuZnSOD. This fact and the presence of glyoxylate-cycle enzymes support the idea that foliar senescence is associated with the transition of peroxisomes into glyoxysomes. Our results indicate that natural senescence causes the same changes in peroxisome-activated oxygen metabolism as dark-induced senescence, and reinforce the hypothesis of an effective role of peroxisomes and their activated oxygen metabolism in this stage of the life cycle.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.113.2.411</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>actividad enzimatica ; activite enzymatique ; Agronomy. Soil science and plant productions ; avejentamiento ; Biological and medical sciences ; catalasa ; catalase ; Cell Biology and Signal Transduction ; cell structure ; Cellular senescence ; ciclo del glioxilato ; cobre ; copper ; cuivre ; cycle du glyoxylate ; cytoplasmic organelles ; darkness ; developmental stages ; Economic plant physiology ; Enzymes ; enzymic activity ; estructura celular ; etapas de desarrollo ; feuille ; Fundamental and applied biological sciences. Psychology ; glyoxylate cycle ; Glyoxysomes ; Growth and development ; hojas ; isocitrate lyase ; isocitrato liasa ; isoenzimas ; isoenzyme ; isoenzymes ; Leaves ; light ; lumiere ; luz ; metabolism ; metabolisme ; metabolismo ; Mitochondria ; mitochondrie ; mitocondria ; Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence ; obscuridad ; obscurite ; organite cellulaire ; organulos citoplasmicos ; oxidoreductases ; oxidorreductasas ; oxigeno ; oxydoreductase ; Oxygen ; Oxygen metabolism ; oxygene ; Peas ; Peroxisomes ; pisum sativum ; Plant physiology and development ; purificacion ; purification ; senescence ; Senescence and abscission ; stade de developpement ; structure cellulaire ; superoxide dismutase ; Superoxides ; superoxido dismutasa ; superoxyde dismutase ; ultraestructura ; ultrastructure ; Vegetative apparatus, growth and morphogenesis. Senescence ; xanthine oxidase ; xanthine oxydase ; xantina oxidasa</subject><ispartof>Plant physiology (Bethesda), 1997-02, Vol.113 (2), p.411-418</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277548$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277548$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2587597$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>PASTORI, G. M</creatorcontrib><creatorcontrib>DEL RIO, L. A</creatorcontrib><creatorcontrib>Estacion Experimental del Zaidin, Granada, Spain</creatorcontrib><creatorcontrib>Eidgenoessische Technische Hochschule Zuerich (Switzerland). Professur Holzwissenschaften</creatorcontrib><title>Natural senescence of pea leaves. An activated oxygen-mediated function for peroxisomes</title><title>Plant physiology (Bethesda)</title><description>We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activities of the O2.- -producing and H2O2-producing enzymes were enhanced by natural senescence. Senescence also produced an increase in the generation of active oxygen species (O2.- and H2O2) in leaf peroxisomes and in the activities of two glyoxylate-cycle marker enzymes. A new fraction of peroxisomes was detected at an advanced stage of dark-induced senescence. Electron microscopy revealed that this new peroxisomal fraction varied in size and electron density. During senescence, the constitutive Mn-superoxide dismutase (SOD) activity of peroxisomes increased and two new CuZn-SODs were induced, one of which cross-reacted with an antibody against glyoxysomal CuZnSOD. This fact and the presence of glyoxylate-cycle enzymes support the idea that foliar senescence is associated with the transition of peroxisomes into glyoxysomes. Our results indicate that natural senescence causes the same changes in peroxisome-activated oxygen metabolism as dark-induced senescence, and reinforce the hypothesis of an effective role of peroxisomes and their activated oxygen metabolism in this stage of the life cycle.</description><subject>actividad enzimatica</subject><subject>activite enzymatique</subject><subject>Agronomy. Soil science and plant productions</subject><subject>avejentamiento</subject><subject>Biological and medical sciences</subject><subject>catalasa</subject><subject>catalase</subject><subject>Cell Biology and Signal Transduction</subject><subject>cell structure</subject><subject>Cellular senescence</subject><subject>ciclo del glioxilato</subject><subject>cobre</subject><subject>copper</subject><subject>cuivre</subject><subject>cycle du glyoxylate</subject><subject>cytoplasmic organelles</subject><subject>darkness</subject><subject>developmental stages</subject><subject>Economic plant physiology</subject><subject>Enzymes</subject><subject>enzymic activity</subject><subject>estructura celular</subject><subject>etapas de desarrollo</subject><subject>feuille</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glyoxylate cycle</subject><subject>Glyoxysomes</subject><subject>Growth and development</subject><subject>hojas</subject><subject>isocitrate lyase</subject><subject>isocitrato liasa</subject><subject>isoenzimas</subject><subject>isoenzyme</subject><subject>isoenzymes</subject><subject>Leaves</subject><subject>light</subject><subject>lumiere</subject><subject>luz</subject><subject>metabolism</subject><subject>metabolisme</subject><subject>metabolismo</subject><subject>Mitochondria</subject><subject>mitochondrie</subject><subject>mitocondria</subject><subject>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</subject><subject>obscuridad</subject><subject>obscurite</subject><subject>organite cellulaire</subject><subject>organulos citoplasmicos</subject><subject>oxidoreductases</subject><subject>oxidorreductasas</subject><subject>oxigeno</subject><subject>oxydoreductase</subject><subject>Oxygen</subject><subject>Oxygen metabolism</subject><subject>oxygene</subject><subject>Peas</subject><subject>Peroxisomes</subject><subject>pisum sativum</subject><subject>Plant physiology and development</subject><subject>purificacion</subject><subject>purification</subject><subject>senescence</subject><subject>Senescence and abscission</subject><subject>stade de developpement</subject><subject>structure cellulaire</subject><subject>superoxide dismutase</subject><subject>Superoxides</subject><subject>superoxido dismutasa</subject><subject>superoxyde dismutase</subject><subject>ultraestructura</subject><subject>ultrastructure</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><subject>xanthine oxidase</subject><subject>xanthine oxydase</subject><subject>xantina oxidasa</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNo9kDtPwzAUhS0EEqWwMSLkgTXBduzaGauKl1TBABVjdXGvq1SpHdlp1f57DEFM9_Gdc4ZDyDVnJedM3nddnlUpSsn5CRlxVYlCKGlOyYixvDNj6nNykdKGMcYrLkfk8xX6XYSWJvSYLHqLNDjaIdAWYY-ppFNPwfbNHnpc0XA4rtEXW1w1v7fb-cyCpy7E7Irh0KSwxXRJzhy0Ca_-5pgsHh8-Zs_F_O3pZTadF06ISV-AqaFCp60FaVjNtV6piUKTn1YwZMAcsxNdYf3ljHIOs95KZ40U2SBUNSZ3Q24HyULrInjbpGUXmy3E41Ioo1Wts-xmkG1SH-I_lkLr3E_GtwN2EJawjjlh8c7rWv-0VGXBN73mZwo</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>PASTORI, G. M</creator><creator>DEL RIO, L. A</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>19970201</creationdate><title>Natural senescence of pea leaves. An activated oxygen-mediated function for peroxisomes</title><author>PASTORI, G. M ; DEL RIO, L. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f226t-a89a3ef7cca4809177d565e8a3ec20e0a0f0c673e9bf85ffe89ac4fc842a48253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>actividad enzimatica</topic><topic>activite enzymatique</topic><topic>Agronomy. Soil science and plant productions</topic><topic>avejentamiento</topic><topic>Biological and medical sciences</topic><topic>catalasa</topic><topic>catalase</topic><topic>Cell Biology and Signal Transduction</topic><topic>cell structure</topic><topic>Cellular senescence</topic><topic>ciclo del glioxilato</topic><topic>cobre</topic><topic>copper</topic><topic>cuivre</topic><topic>cycle du glyoxylate</topic><topic>cytoplasmic organelles</topic><topic>darkness</topic><topic>developmental stages</topic><topic>Economic plant physiology</topic><topic>Enzymes</topic><topic>enzymic activity</topic><topic>estructura celular</topic><topic>etapas de desarrollo</topic><topic>feuille</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glyoxylate cycle</topic><topic>Glyoxysomes</topic><topic>Growth and development</topic><topic>hojas</topic><topic>isocitrate lyase</topic><topic>isocitrato liasa</topic><topic>isoenzimas</topic><topic>isoenzyme</topic><topic>isoenzymes</topic><topic>Leaves</topic><topic>light</topic><topic>lumiere</topic><topic>luz</topic><topic>metabolism</topic><topic>metabolisme</topic><topic>metabolismo</topic><topic>Mitochondria</topic><topic>mitochondrie</topic><topic>mitocondria</topic><topic>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</topic><topic>obscuridad</topic><topic>obscurite</topic><topic>organite cellulaire</topic><topic>organulos citoplasmicos</topic><topic>oxidoreductases</topic><topic>oxidorreductasas</topic><topic>oxigeno</topic><topic>oxydoreductase</topic><topic>Oxygen</topic><topic>Oxygen metabolism</topic><topic>oxygene</topic><topic>Peas</topic><topic>Peroxisomes</topic><topic>pisum sativum</topic><topic>Plant physiology and development</topic><topic>purificacion</topic><topic>purification</topic><topic>senescence</topic><topic>Senescence and abscission</topic><topic>stade de developpement</topic><topic>structure cellulaire</topic><topic>superoxide dismutase</topic><topic>Superoxides</topic><topic>superoxido dismutasa</topic><topic>superoxyde dismutase</topic><topic>ultraestructura</topic><topic>ultrastructure</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><topic>xanthine oxidase</topic><topic>xanthine oxydase</topic><topic>xantina oxidasa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PASTORI, G. M</creatorcontrib><creatorcontrib>DEL RIO, L. A</creatorcontrib><creatorcontrib>Estacion Experimental del Zaidin, Granada, Spain</creatorcontrib><creatorcontrib>Eidgenoessische Technische Hochschule Zuerich (Switzerland). Professur Holzwissenschaften</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PASTORI, G. M</au><au>DEL RIO, L. A</au><aucorp>Estacion Experimental del Zaidin, Granada, Spain</aucorp><aucorp>Eidgenoessische Technische Hochschule Zuerich (Switzerland). Professur Holzwissenschaften</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural senescence of pea leaves. An activated oxygen-mediated function for peroxisomes</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>1997-02-01</date><risdate>1997</risdate><volume>113</volume><issue>2</issue><spage>411</spage><epage>418</epage><pages>411-418</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activities of the O2.- -producing and H2O2-producing enzymes were enhanced by natural senescence. Senescence also produced an increase in the generation of active oxygen species (O2.- and H2O2) in leaf peroxisomes and in the activities of two glyoxylate-cycle marker enzymes. A new fraction of peroxisomes was detected at an advanced stage of dark-induced senescence. Electron microscopy revealed that this new peroxisomal fraction varied in size and electron density. During senescence, the constitutive Mn-superoxide dismutase (SOD) activity of peroxisomes increased and two new CuZn-SODs were induced, one of which cross-reacted with an antibody against glyoxysomal CuZnSOD. This fact and the presence of glyoxylate-cycle enzymes support the idea that foliar senescence is associated with the transition of peroxisomes into glyoxysomes. Our results indicate that natural senescence causes the same changes in peroxisome-activated oxygen metabolism as dark-induced senescence, and reinforce the hypothesis of an effective role of peroxisomes and their activated oxygen metabolism in this stage of the life cycle.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><doi>10.1104/pp.113.2.411</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1997-02, Vol.113 (2), p.411-418 |
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| source | JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | actividad enzimatica activite enzymatique Agronomy. Soil science and plant productions avejentamiento Biological and medical sciences catalasa catalase Cell Biology and Signal Transduction cell structure Cellular senescence ciclo del glioxilato cobre copper cuivre cycle du glyoxylate cytoplasmic organelles darkness developmental stages Economic plant physiology Enzymes enzymic activity estructura celular etapas de desarrollo feuille Fundamental and applied biological sciences. Psychology glyoxylate cycle Glyoxysomes Growth and development hojas isocitrate lyase isocitrato liasa isoenzimas isoenzyme isoenzymes Leaves light lumiere luz metabolism metabolisme metabolismo Mitochondria mitochondrie mitocondria Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence obscuridad obscurite organite cellulaire organulos citoplasmicos oxidoreductases oxidorreductasas oxigeno oxydoreductase Oxygen Oxygen metabolism oxygene Peas Peroxisomes pisum sativum Plant physiology and development purificacion purification senescence Senescence and abscission stade de developpement structure cellulaire superoxide dismutase Superoxides superoxido dismutasa superoxyde dismutase ultraestructura ultrastructure Vegetative apparatus, growth and morphogenesis. Senescence xanthine oxidase xanthine oxydase xantina oxidasa |
| title | Natural senescence of pea leaves. An activated oxygen-mediated function for peroxisomes |
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