Regeneration of transgenic papaya plants via somatic embryogenesis induced by Agrobacterium rhizogenes
An Agrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed roots, induced from leaf discs infected with A. rhizogenes. Transformation was monitore...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 1996-04, Vol.32 (2), p.86-90 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.) Vegas-Garcia, A Herrera-Estrella, L |
| description | An Agrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed roots, induced from leaf discs infected with A. rhizogenes. Transformation was monitored by autonomous growth of roots and somatic embryos, resistance to kanamycin, β-glucuronidase activity (GUS), and Southern hybridization analysis. Over one-third of the infected leaf explants produced transformed roots with GUS activity, from which 10% spontaneously produced somatic embryos. Histological analysis of A. rhizogenes-transformed embryos showed that they have an altered symmetry between the shoot apex and the root meristem when compared to somatic embryos induced with hormone treatment from control explants. Transgenic papaya plants containing A. rhizogenes rol genes were more sensitive to auxins, developed wrinkled leaves, and grew slower than nontransformed plants. |
| doi_str_mv | 10.1007/BF02823136 |
| format | Article |
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(Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.) ; Vegas-Garcia, A ; Herrera-Estrella, L</creator><creatorcontrib>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.) ; Vegas-Garcia, A ; Herrera-Estrella, L</creatorcontrib><description>An Agrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed roots, induced from leaf discs infected with A. rhizogenes. Transformation was monitored by autonomous growth of roots and somatic embryos, resistance to kanamycin, β-glucuronidase activity (GUS), and Southern hybridization analysis. Over one-third of the infected leaf explants produced transformed roots with GUS activity, from which 10% spontaneously produced somatic embryos. Histological analysis of A. rhizogenes-transformed embryos showed that they have an altered symmetry between the shoot apex and the root meristem when compared to somatic embryos induced with hormone treatment from control explants. Transgenic papaya plants containing A. rhizogenes rol genes were more sensitive to auxins, developed wrinkled leaves, and grew slower than nontransformed plants.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02823136</identifier><language>eng</language><publisher>Wallingford: Society for In Vitro Biology</publisher><subject>ADN ; Agrobacterium ; AGROBACTERIUM RHIZOGENES ; Agronomy. Soil science and plant productions ; Auxins ; BETA-GLUCURONIDASE ; Biological and medical sciences ; Biotechnology ; Callus ; CARICA PAPAYA ; CULTIVO DE TEJIDOS ; CULTIVO IN VITRO ; CULTURE DE TISSU ; CULTURE IN VITRO ; DNA ; EMBRIONES VEGETALES ; EMBRYON VEGETAL ; Embryos ; EXPLANT ; EXPLANTES ; EXPLANTS ; FEUILLE ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; GENETIC TRANSFORMATION ; Genetic Transformation/Somatic Cell Genetics ; Genetics and breeding of economic plants ; GLICOSIDASAS ; GLYCOSIDASE ; GLYCOSIDASES ; HISTOENZYMOLOGY ; HOJAS ; IN VITRO CULTURE ; IN VITRO REGENERATION ; LEAVES ; Methods. Procedures. Technologies ; MICROPROPAGACION ; MICROPROPAGATION ; Plant breeding: fundamental aspects and methodology ; Plant cells ; PLANT EMBRYOS ; Plant roots ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; REGENERACION IN VITRO ; REGENERATION IN VITRO ; RI PLASMID ; Somatic embryogenesis ; Somatic embryos ; TISSUE CULTURE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Transgenic animals and transgenic plants ; TRANSGENIC PLANTS</subject><ispartof>In vitro cellular & developmental biology. Plant, 1996-04, Vol.32 (2), p.86-90</ispartof><rights>Copyright 1996 Society for In Vitro Biology</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-178652ce2cc31c6dcb470c511f463a8290ab2487120b2a1afe21ef064e5ed8723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20064883$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20064883$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2476096$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.)</creatorcontrib><creatorcontrib>Vegas-Garcia, A</creatorcontrib><creatorcontrib>Herrera-Estrella, L</creatorcontrib><title>Regeneration of transgenic papaya plants via somatic embryogenesis induced by Agrobacterium rhizogenes</title><title>In vitro cellular & developmental biology. Plant</title><description>An Agrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed roots, induced from leaf discs infected with A. rhizogenes. Transformation was monitored by autonomous growth of roots and somatic embryos, resistance to kanamycin, β-glucuronidase activity (GUS), and Southern hybridization analysis. Over one-third of the infected leaf explants produced transformed roots with GUS activity, from which 10% spontaneously produced somatic embryos. Histological analysis of A. rhizogenes-transformed embryos showed that they have an altered symmetry between the shoot apex and the root meristem when compared to somatic embryos induced with hormone treatment from control explants. Transgenic papaya plants containing A. rhizogenes rol genes were more sensitive to auxins, developed wrinkled leaves, and grew slower than nontransformed plants.</description><subject>ADN</subject><subject>Agrobacterium</subject><subject>AGROBACTERIUM RHIZOGENES</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Auxins</subject><subject>BETA-GLUCURONIDASE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Callus</subject><subject>CARICA PAPAYA</subject><subject>CULTIVO DE TEJIDOS</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE DE TISSU</subject><subject>CULTURE IN VITRO</subject><subject>DNA</subject><subject>EMBRIONES VEGETALES</subject><subject>EMBRYON VEGETAL</subject><subject>Embryos</subject><subject>EXPLANT</subject><subject>EXPLANTES</subject><subject>EXPLANTS</subject><subject>FEUILLE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genetic Transformation/Somatic Cell Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>GLICOSIDASAS</subject><subject>GLYCOSIDASE</subject><subject>GLYCOSIDASES</subject><subject>HISTOENZYMOLOGY</subject><subject>HOJAS</subject><subject>IN VITRO CULTURE</subject><subject>IN VITRO REGENERATION</subject><subject>LEAVES</subject><subject>Methods. Procedures. Technologies</subject><subject>MICROPROPAGACION</subject><subject>MICROPROPAGATION</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells</subject><subject>PLANT EMBRYOS</subject><subject>Plant roots</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>REGENERACION IN VITRO</subject><subject>REGENERATION IN VITRO</subject><subject>RI PLASMID</subject><subject>Somatic embryogenesis</subject><subject>Somatic embryos</subject><subject>TISSUE CULTURE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Transgenic animals and transgenic plants</subject><subject>TRANSGENIC PLANTS</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEQhoMoWKsXj4KQg9fVfCd7rGJVKAhqz2U2m9SU7gfJVlh_vZEVPM0w7zMPzCB0ScktJUTf3S8JM4xTro7QjAotC6ZMeZx7IkUhhVan6CylHSGEEqpnyL-5rWtdhCF0Le48HiK0KY-CxT30MALu99AOCX8FwKlrMmixa6o4dr-LKSQc2vpgXY2rES-2savADi6GQ4PjZ_ieqHN04mGf3MVfnaP18vHj4blYvT69PCxWhWVKDAXVRklmHbOWU6tqWwlNrKTUC8XBsJJAxYTRlJGKAQXvGHWeKOGkq41mfI5uJm8PycLe52NsSJs-hgbiuGH5AaRUGbuesF0auvgfk6wyhuf8aso9dBvYxqxYv5eKlkxy_gNXamtd</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.)</creator><creator>Vegas-Garcia, A</creator><creator>Herrera-Estrella, L</creator><general>Society for In Vitro Biology</general><general>Cambridge University Press</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>19960401</creationdate><title>Regeneration of transgenic papaya plants via somatic embryogenesis induced by Agrobacterium rhizogenes</title><author>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.) ; Vegas-Garcia, A ; Herrera-Estrella, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-178652ce2cc31c6dcb470c511f463a8290ab2487120b2a1afe21ef064e5ed8723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADN</topic><topic>Agrobacterium</topic><topic>AGROBACTERIUM RHIZOGENES</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Auxins</topic><topic>BETA-GLUCURONIDASE</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Callus</topic><topic>CARICA PAPAYA</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE DE TISSU</topic><topic>CULTURE IN VITRO</topic><topic>DNA</topic><topic>EMBRIONES VEGETALES</topic><topic>EMBRYON VEGETAL</topic><topic>Embryos</topic><topic>EXPLANT</topic><topic>EXPLANTES</topic><topic>EXPLANTS</topic><topic>FEUILLE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genetic Transformation/Somatic Cell Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>GLICOSIDASAS</topic><topic>GLYCOSIDASE</topic><topic>GLYCOSIDASES</topic><topic>HISTOENZYMOLOGY</topic><topic>HOJAS</topic><topic>IN VITRO CULTURE</topic><topic>IN VITRO REGENERATION</topic><topic>LEAVES</topic><topic>Methods. Procedures. Technologies</topic><topic>MICROPROPAGACION</topic><topic>MICROPROPAGATION</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant cells</topic><topic>PLANT EMBRYOS</topic><topic>Plant roots</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>REGENERACION IN VITRO</topic><topic>REGENERATION IN VITRO</topic><topic>RI PLASMID</topic><topic>Somatic embryogenesis</topic><topic>Somatic embryos</topic><topic>TISSUE CULTURE</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Transgenic animals and transgenic plants</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.)</creatorcontrib><creatorcontrib>Vegas-Garcia, A</creatorcontrib><creatorcontrib>Herrera-Estrella, L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cabrera-Ponce, J.L. (Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Mexico.)</au><au>Vegas-Garcia, A</au><au>Herrera-Estrella, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regeneration of transgenic papaya plants via somatic embryogenesis induced by Agrobacterium rhizogenes</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>1996-04-01</date><risdate>1996</risdate><volume>32</volume><issue>2</issue><spage>86</spage><epage>90</epage><pages>86-90</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>An Agrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed roots, induced from leaf discs infected with A. rhizogenes. Transformation was monitored by autonomous growth of roots and somatic embryos, resistance to kanamycin, β-glucuronidase activity (GUS), and Southern hybridization analysis. Over one-third of the infected leaf explants produced transformed roots with GUS activity, from which 10% spontaneously produced somatic embryos. Histological analysis of A. rhizogenes-transformed embryos showed that they have an altered symmetry between the shoot apex and the root meristem when compared to somatic embryos induced with hormone treatment from control explants. Transgenic papaya plants containing A. rhizogenes rol genes were more sensitive to auxins, developed wrinkled leaves, and grew slower than nontransformed plants.</abstract><cop>Wallingford</cop><pub>Society for In Vitro Biology</pub><doi>10.1007/BF02823136</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 1054-5476 |
| ispartof | In vitro cellular & developmental biology. Plant, 1996-04, Vol.32 (2), p.86-90 |
| issn | 1054-5476 1475-2689 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_2476096 |
| source | JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | ADN Agrobacterium AGROBACTERIUM RHIZOGENES Agronomy. Soil science and plant productions Auxins BETA-GLUCURONIDASE Biological and medical sciences Biotechnology Callus CARICA PAPAYA CULTIVO DE TEJIDOS CULTIVO IN VITRO CULTURE DE TISSU CULTURE IN VITRO DNA EMBRIONES VEGETALES EMBRYON VEGETAL Embryos EXPLANT EXPLANTES EXPLANTS FEUILLE Fundamental and applied biological sciences. Psychology Genetic engineering Genetic engineering applications Genetic technics GENETIC TRANSFORMATION Genetic Transformation/Somatic Cell Genetics Genetics and breeding of economic plants GLICOSIDASAS GLYCOSIDASE GLYCOSIDASES HISTOENZYMOLOGY HOJAS IN VITRO CULTURE IN VITRO REGENERATION LEAVES Methods. Procedures. Technologies MICROPROPAGACION MICROPROPAGATION Plant breeding: fundamental aspects and methodology Plant cells PLANT EMBRYOS Plant roots PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants PLASMIDE PLASMIDIOS PLASMIDS REGENERACION IN VITRO REGENERATION IN VITRO RI PLASMID Somatic embryogenesis Somatic embryos TISSUE CULTURE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenic animals and transgenic plants TRANSGENIC PLANTS |
| title | Regeneration of transgenic papaya plants via somatic embryogenesis induced by Agrobacterium rhizogenes |
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