Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae

A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abp1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The...

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Veröffentlicht in:	Applied and Environmental Microbiology 1996-10, Vol.62 (10), p.3840-3846
Hauptverfasser:	Margolles-Clark, E. (VTT Biotechnology and Food Research, Espoo, Finland.), Tenkanen, M, Nakari-Setala, T, Penttila, M
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence Arabinose - metabolism Base Sequence Biological and medical sciences Biotechnology CLONACION CLONAGE Cloning, Molecular Enzymes EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE Gene Expression Gene Library GENES Genes, Fungal - genetics Genetic engineering Genetic technics Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism HIDROLASAS HIDROLISIS HYDROLASE HYDROLYSE Methods. Procedures. Technologies Microbiology Molecular cloning Molecular Sequence Data SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics SECUENCIA NUCLEOTIDICA Sequence Alignment Sequence Analysis Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Trichoderma - enzymology Trichoderma - genetics TRICHODERMA LONGIBRACHIATUM Trichoderma reesei XILANOS XYLANE Xylans - metabolism Xylose - metabolism Xylosidases - genetics Xylosidases - metabolism Yeast
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