Apoplastic ascorbate does not prevent the oxidation of fluorescent amphiphilic dyes by ambient and elevated concentrations of ozone in leaves

Cut leaves of spinach were infiltrated with solutions containing oxidation-sensitive fluorescent dyes. Excess solution was removed from the intercellular space by centrifugation. Fluorescence of the dyes D494 and D283 started to decrease immediately after the onset of fumigation with ozone at concen...

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