Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung
Antenatal administration of triiodo-L-thyronine (T3) to late gestation rats resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no rep...
Gespeichert in:
| Veröffentlicht in: | Experimental lung research 1998, Vol.24 (5), p.641-657 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 657 |
|---|---|
| container_issue | 5 |
| container_start_page | 641 |
| container_title | Experimental lung research |
| container_volume | 24 |
| creator | Ramadurai, Sujatha M. Nielsen, Heber C. Chen, Youwei Hatzis, Dimitrios Sosenko, Llene R. S. |
| description | Antenatal administration of triiodo-L-thyronine (T3) to late gestation rats resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no reported studies of the simultaneous in vivo developmental influence of T3 on both pulmonary AOE and SP gene expression. We hypothesized that antenatal T3 treatment would cause differential regulation of surfactant phospholipid, SP, and AOE genes in the late gestation fetal rat. Timed pregnant rats received intramuscular injections of either T3 (7 mg/kg) or placebo on days 19 and 20 of gestation and fetuses were delivered on day 21. Fetal lung SP-A, SP-B, SP-C, and AOE mRNA levels were studied by Northern analysis. AOE mRNA levels were further quantitated by solution hybridization. Total lung phospholipids (TPL) and disaturated phosphatidylcholine (DSPC) content were quantitated by a phosphorus assay. T3 significantly increased TPL and DSPC content, and significantly decreased the expression of SP-A, SP-C, CuZnSOD, and catalase genes. Because of a crucial interplay of these factors for normal lung function at the time of birth, the molecular mechanisms by which these apparently opposing changes are accomplished warrant further investigation |
| doi_str_mv | 10.3109/01902149809099585 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_2393767</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69972942</sourcerecordid><originalsourceid>FETCH-LOGICAL-c430t-233b859ff95b6aef490f9b8e69b38a129d2c6d03bdb2151b51d7e97224ef1eed3</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EKtPCA7BA8gJ1RcA_-bNgMypTWmkEqBS2kRNfE1eJHWyn7fA6vCgezbRShdSVbZ3vHF_7IPSKknecEvGeUEEYzUVNBBGiqIsnaEELRjOSC_EULbZ6loD6OToM4YoQwoq6PEAHoqoEr_IF-vvJaA0ebDRywKu072LAxuKf5tphp_Flv_HOKHzm_OgsYGdx7AGvbicPIZh0TND32WvZRWkj_ta7MPVuMJNRbx8I3kVIwePFlyWWVuFlutPdGrUVV_bPZoSdlphTiGmaCxnxera_XqBnWg4BXu7XI_TjdHV5cpatv34-P1musy7nJGaM87YuhNaiaEsJOhdEi7aGUrS8lpQJxbpSEd6qltGCtgVVFYiKsRw0BVD8CB3vcifvfs8QYjOa0MEwSAtuDk0pEi1ylkC6AzvvQvCgm8mbUfpNQ0mzLab5r5jkeb0Pn9sR1L1j30TS3-x1GTo5aC9tZ8I9xniiyiphH3eYsTo1Im-cH1QT5WZw_s7DH5viwwN7D3KIfSc9NFdu9jZ97yNv-AenzLza</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69972942</pqid></control><display><type>article</type><title>Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung</title><source>MEDLINE</source><source>Taylor & Francis:Master (3349 titles)</source><creator>Ramadurai, Sujatha M. ; Nielsen, Heber C. ; Chen, Youwei ; Hatzis, Dimitrios ; Sosenko, Llene R. S.</creator><creatorcontrib>Ramadurai, Sujatha M. ; Nielsen, Heber C. ; Chen, Youwei ; Hatzis, Dimitrios ; Sosenko, Llene R. S.</creatorcontrib><description>Antenatal administration of triiodo-L-thyronine (T3) to late gestation rats resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no reported studies of the simultaneous in vivo developmental influence of T3 on both pulmonary AOE and SP gene expression. We hypothesized that antenatal T3 treatment would cause differential regulation of surfactant phospholipid, SP, and AOE genes in the late gestation fetal rat. Timed pregnant rats received intramuscular injections of either T3 (7 mg/kg) or placebo on days 19 and 20 of gestation and fetuses were delivered on day 21. Fetal lung SP-A, SP-B, SP-C, and AOE mRNA levels were studied by Northern analysis. AOE mRNA levels were further quantitated by solution hybridization. Total lung phospholipids (TPL) and disaturated phosphatidylcholine (DSPC) content were quantitated by a phosphorus assay. T3 significantly increased TPL and DSPC content, and significantly decreased the expression of SP-A, SP-C, CuZnSOD, and catalase genes. Because of a crucial interplay of these factors for normal lung function at the time of birth, the molecular mechanisms by which these apparently opposing changes are accomplished warrant further investigation</description><identifier>ISSN: 0190-2148</identifier><identifier>EISSN: 1521-0499</identifier><identifier>DOI: 10.3109/01902149809099585</identifier><identifier>PMID: 9779374</identifier><identifier>CODEN: EXLRDA</identifier><language>eng</language><publisher>Philadelphia, PA: Informa UK Ltd</publisher><subject>Air breathing ; Animals ; antioxidant enzymes (ADE) ; Biological and medical sciences ; Blotting, Northern ; Catalase - genetics ; Catalase - metabolism ; DNA Primers - chemistry ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Glutathione Peroxidase - genetics ; Glutathione Peroxidase - metabolism ; Lung - drug effects ; Lung - embryology ; Lung - metabolism ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Phosphatidylcholines - metabolism ; Phospholipids - genetics ; Phospholipids - metabolism ; Polymerase Chain Reaction ; Pregnancy ; Pulmonary Surfactants - genetics ; Pulmonary Surfactants - metabolism ; Rats ; Rats, Sprague-Dawley ; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics ; Ribosomal Proteins - metabolism ; RNA, Messenger - metabolism ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; surfactant protein genes (SP) ; tri-iodothyronine (T ; Triiodothyronine - pharmacology ; Vertebrates: respiratory system</subject><ispartof>Experimental lung research, 1998, Vol.24 (5), p.641-657</ispartof><rights>1998 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-233b859ff95b6aef490f9b8e69b38a129d2c6d03bdb2151b51d7e97224ef1eed3</citedby><cites>FETCH-LOGICAL-c430t-233b859ff95b6aef490f9b8e69b38a129d2c6d03bdb2151b51d7e97224ef1eed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/01902149809099585$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/01902149809099585$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2393767$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9779374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramadurai, Sujatha M.</creatorcontrib><creatorcontrib>Nielsen, Heber C.</creatorcontrib><creatorcontrib>Chen, Youwei</creatorcontrib><creatorcontrib>Hatzis, Dimitrios</creatorcontrib><creatorcontrib>Sosenko, Llene R. S.</creatorcontrib><title>Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung</title><title>Experimental lung research</title><addtitle>Exp Lung Res</addtitle><description>Antenatal administration of triiodo-L-thyronine (T3) to late gestation rats resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no reported studies of the simultaneous in vivo developmental influence of T3 on both pulmonary AOE and SP gene expression. We hypothesized that antenatal T3 treatment would cause differential regulation of surfactant phospholipid, SP, and AOE genes in the late gestation fetal rat. Timed pregnant rats received intramuscular injections of either T3 (7 mg/kg) or placebo on days 19 and 20 of gestation and fetuses were delivered on day 21. Fetal lung SP-A, SP-B, SP-C, and AOE mRNA levels were studied by Northern analysis. AOE mRNA levels were further quantitated by solution hybridization. Total lung phospholipids (TPL) and disaturated phosphatidylcholine (DSPC) content were quantitated by a phosphorus assay. T3 significantly increased TPL and DSPC content, and significantly decreased the expression of SP-A, SP-C, CuZnSOD, and catalase genes. Because of a crucial interplay of these factors for normal lung function at the time of birth, the molecular mechanisms by which these apparently opposing changes are accomplished warrant further investigation</description><subject>Air breathing</subject><subject>Animals</subject><subject>antioxidant enzymes (ADE)</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Catalase - genetics</subject><subject>Catalase - metabolism</subject><subject>DNA Primers - chemistry</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Glutathione Peroxidase - genetics</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Lung - drug effects</subject><subject>Lung - embryology</subject><subject>Lung - metabolism</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phospholipids - genetics</subject><subject>Phospholipids - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Pulmonary Surfactants - genetics</subject><subject>Pulmonary Surfactants - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</subject><subject>Ribosomal Proteins - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>surfactant protein genes (SP)</subject><subject>tri-iodothyronine (T</subject><subject>Triiodothyronine - pharmacology</subject><subject>Vertebrates: respiratory system</subject><issn>0190-2148</issn><issn>1521-0499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EKtPCA7BA8gJ1RcA_-bNgMypTWmkEqBS2kRNfE1eJHWyn7fA6vCgezbRShdSVbZ3vHF_7IPSKknecEvGeUEEYzUVNBBGiqIsnaEELRjOSC_EULbZ6loD6OToM4YoQwoq6PEAHoqoEr_IF-vvJaA0ebDRywKu072LAxuKf5tphp_Flv_HOKHzm_OgsYGdx7AGvbicPIZh0TND32WvZRWkj_ta7MPVuMJNRbx8I3kVIwePFlyWWVuFlutPdGrUVV_bPZoSdlphTiGmaCxnxera_XqBnWg4BXu7XI_TjdHV5cpatv34-P1musy7nJGaM87YuhNaiaEsJOhdEi7aGUrS8lpQJxbpSEd6qltGCtgVVFYiKsRw0BVD8CB3vcifvfs8QYjOa0MEwSAtuDk0pEi1ylkC6AzvvQvCgm8mbUfpNQ0mzLab5r5jkeb0Pn9sR1L1j30TS3-x1GTo5aC9tZ8I9xniiyiphH3eYsTo1Im-cH1QT5WZw_s7DH5viwwN7D3KIfSc9NFdu9jZ97yNv-AenzLza</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Ramadurai, Sujatha M.</creator><creator>Nielsen, Heber C.</creator><creator>Chen, Youwei</creator><creator>Hatzis, Dimitrios</creator><creator>Sosenko, Llene R. S.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung</title><author>Ramadurai, Sujatha M. ; Nielsen, Heber C. ; Chen, Youwei ; Hatzis, Dimitrios ; Sosenko, Llene R. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-233b859ff95b6aef490f9b8e69b38a129d2c6d03bdb2151b51d7e97224ef1eed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Air breathing</topic><topic>Animals</topic><topic>antioxidant enzymes (ADE)</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Catalase - genetics</topic><topic>Catalase - metabolism</topic><topic>DNA Primers - chemistry</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Glutathione Peroxidase - genetics</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Lung - drug effects</topic><topic>Lung - embryology</topic><topic>Lung - metabolism</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phospholipids - genetics</topic><topic>Phospholipids - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Pulmonary Surfactants - genetics</topic><topic>Pulmonary Surfactants - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</topic><topic>Ribosomal Proteins - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>surfactant protein genes (SP)</topic><topic>tri-iodothyronine (T</topic><topic>Triiodothyronine - pharmacology</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramadurai, Sujatha M.</creatorcontrib><creatorcontrib>Nielsen, Heber C.</creatorcontrib><creatorcontrib>Chen, Youwei</creatorcontrib><creatorcontrib>Hatzis, Dimitrios</creatorcontrib><creatorcontrib>Sosenko, Llene R. S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental lung research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramadurai, Sujatha M.</au><au>Nielsen, Heber C.</au><au>Chen, Youwei</au><au>Hatzis, Dimitrios</au><au>Sosenko, Llene R. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung</atitle><jtitle>Experimental lung research</jtitle><addtitle>Exp Lung Res</addtitle><date>1998</date><risdate>1998</risdate><volume>24</volume><issue>5</issue><spage>641</spage><epage>657</epage><pages>641-657</pages><issn>0190-2148</issn><eissn>1521-0499</eissn><coden>EXLRDA</coden><abstract>Antenatal administration of triiodo-L-thyronine (T3) to late gestation rats resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no reported studies of the simultaneous in vivo developmental influence of T3 on both pulmonary AOE and SP gene expression. We hypothesized that antenatal T3 treatment would cause differential regulation of surfactant phospholipid, SP, and AOE genes in the late gestation fetal rat. Timed pregnant rats received intramuscular injections of either T3 (7 mg/kg) or placebo on days 19 and 20 of gestation and fetuses were delivered on day 21. Fetal lung SP-A, SP-B, SP-C, and AOE mRNA levels were studied by Northern analysis. AOE mRNA levels were further quantitated by solution hybridization. Total lung phospholipids (TPL) and disaturated phosphatidylcholine (DSPC) content were quantitated by a phosphorus assay. T3 significantly increased TPL and DSPC content, and significantly decreased the expression of SP-A, SP-C, CuZnSOD, and catalase genes. Because of a crucial interplay of these factors for normal lung function at the time of birth, the molecular mechanisms by which these apparently opposing changes are accomplished warrant further investigation</abstract><cop>Philadelphia, PA</cop><pub>Informa UK Ltd</pub><pmid>9779374</pmid><doi>10.3109/01902149809099585</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0190-2148 |
| ispartof | Experimental lung research, 1998, Vol.24 (5), p.641-657 |
| issn | 0190-2148 1521-0499 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_2393767 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles) |
| subjects | Air breathing Animals antioxidant enzymes (ADE) Biological and medical sciences Blotting, Northern Catalase - genetics Catalase - metabolism DNA Primers - chemistry Female Fundamental and applied biological sciences. Psychology Gene Expression Glutathione Peroxidase - genetics Glutathione Peroxidase - metabolism Lung - drug effects Lung - embryology Lung - metabolism Oxidoreductases - genetics Oxidoreductases - metabolism Phosphatidylcholines - metabolism Phospholipids - genetics Phospholipids - metabolism Polymerase Chain Reaction Pregnancy Pulmonary Surfactants - genetics Pulmonary Surfactants - metabolism Rats Rats, Sprague-Dawley Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics Ribosomal Proteins - metabolism RNA, Messenger - metabolism Superoxide Dismutase - genetics Superoxide Dismutase - metabolism surfactant protein genes (SP) tri-iodothyronine (T Triiodothyronine - pharmacology Vertebrates: respiratory system |
| title | Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T21%3A11%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20Effects%20in%20Vivo%20of%20Thyroid%20Hormone%20on%20the%20Expression%20of%20Surfactant%20Phospholipid,%20Surfactant%20Protein%20mRNA%20and%20Antioxidant%20Enzyme%20mRNA%20in%20Fetal%20Rat%20Lung&rft.jtitle=Experimental%20lung%20research&rft.au=Ramadurai,%20Sujatha%20M.&rft.date=1998&rft.volume=24&rft.issue=5&rft.spage=641&rft.epage=657&rft.pages=641-657&rft.issn=0190-2148&rft.eissn=1521-0499&rft.coden=EXLRDA&rft_id=info:doi/10.3109/01902149809099585&rft_dat=%3Cproquest_pasca%3E69972942%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69972942&rft_id=info:pmid/9779374&rfr_iscdi=true |