Sequence analysis by cloning of the structural gene of gassericin A, a hydrophobic bacteriocin produced by Lactobacillus gasseri LA39
Gassericin A, a bacteriocin from Lactobacillus gasseri LA39, was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis,unlike the estimated 3,800 found by SDS-PAGE. However, when the pur...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-05, Vol.62 (5), p.887-892 |
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| creator | Kawai, Y. (Tohoku Univ., Sendai (Japan)) Saito, T Suzuki, M Itoh, T |
| description | Gassericin A, a bacteriocin from Lactobacillus gasseri LA39, was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis,unlike the estimated 3,800 found by SDS-PAGE. However, when the purified preparation was treated with lysylendopeptidase, it migrated as a single band to 5,600 with bacteriocin activity on SDS-PAGE. N- and C-terminal amino acids could not be identified. The internal amino acid could be identified after gassericin A was hydrolyzed with lysylendopeptidase. The DNA of the structural gene of gassericin A was sequenced by cloning of the gene from chromosomal DNA with an oligonucleotide probe. The structural gene of gassericin A was found on the chromosomal DNA as an open reading frame encoding a protein composed of 91 amino acids. The amino acid sequence of mature gassericin A was predicted to be 58 residues from the DNA sequence and results of mass analysis. These results suggested that gassericin A has a closed circular structure with N- and C-terminals linked. Gassericin A is a hydrophobic class II bacteriocin; it was 98% identical with acidocin B produced by Lactobacillus acidophilus M46 |
| doi_str_mv | 10.1271/bbb.62.887 |
| format | Article |
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(Tohoku Univ., Sendai (Japan)) ; Saito, T ; Suzuki, M ; Itoh, T</creator><creatorcontrib>Kawai, Y. (Tohoku Univ., Sendai (Japan)) ; Saito, T ; Suzuki, M ; Itoh, T</creatorcontrib><description>Gassericin A, a bacteriocin from Lactobacillus gasseri LA39, was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis,unlike the estimated 3,800 found by SDS-PAGE. However, when the purified preparation was treated with lysylendopeptidase, it migrated as a single band to 5,600 with bacteriocin activity on SDS-PAGE. N- and C-terminal amino acids could not be identified. The internal amino acid could be identified after gassericin A was hydrolyzed with lysylendopeptidase. The DNA of the structural gene of gassericin A was sequenced by cloning of the gene from chromosomal DNA with an oligonucleotide probe. The structural gene of gassericin A was found on the chromosomal DNA as an open reading frame encoding a protein composed of 91 amino acids. The amino acid sequence of mature gassericin A was predicted to be 58 residues from the DNA sequence and results of mass analysis. These results suggested that gassericin A has a closed circular structure with N- and C-terminals linked. Gassericin A is a hydrophobic class II bacteriocin; it was 98% identical with acidocin B produced by Lactobacillus acidophilus M46</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.62.887</identifier><identifier>PMID: 9648219</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>acidocin B ; Amino Acid Sequence ; Animals ; bacteriocin ; BACTERIOCINAS ; BACTERIOCINE ; BACTERIOCINS ; Bacteriocins - chemistry ; Bacteriocins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. Psychology ; gassericin A ; GENE ; Gene Expression Regulation, Bacterial ; GENES ; HIDROFOBICIDAD ; Humans ; HYDROPHOBICITE ; HYDROPHOBICITY ; Infant ; lactic acid bacteria ; LACTOBACILLUS ; Lactobacillus - metabolism ; Lactobacillus gasseri ; Microbiology ; Mission oriented research ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; PEPTIDE ; PEPTIDES ; PEPTIDOS ; Physiology and metabolism ; PURIFICACION ; PURIFICATION ; SECUENCIA NUCLEOTIDICA ; Sequence Analysis, DNA ; SEQUENCE NUCLEOTIDIQUE</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998-05, Vol.62 (5), p.887-892</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c564t-ec398436e3171c94d2943b8b4154eebcb4fe20a8e168764f99e9010601a0ee423</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2348892$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9648219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawai, Y. (Tohoku Univ., Sendai (Japan))</creatorcontrib><creatorcontrib>Saito, T</creatorcontrib><creatorcontrib>Suzuki, M</creatorcontrib><creatorcontrib>Itoh, T</creatorcontrib><title>Sequence analysis by cloning of the structural gene of gassericin A, a hydrophobic bacteriocin produced by Lactobacillus gasseri LA39</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Gassericin A, a bacteriocin from Lactobacillus gasseri LA39, was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis,unlike the estimated 3,800 found by SDS-PAGE. However, when the purified preparation was treated with lysylendopeptidase, it migrated as a single band to 5,600 with bacteriocin activity on SDS-PAGE. N- and C-terminal amino acids could not be identified. The internal amino acid could be identified after gassericin A was hydrolyzed with lysylendopeptidase. The DNA of the structural gene of gassericin A was sequenced by cloning of the gene from chromosomal DNA with an oligonucleotide probe. The structural gene of gassericin A was found on the chromosomal DNA as an open reading frame encoding a protein composed of 91 amino acids. The amino acid sequence of mature gassericin A was predicted to be 58 residues from the DNA sequence and results of mass analysis. These results suggested that gassericin A has a closed circular structure with N- and C-terminals linked. Gassericin A is a hydrophobic class II bacteriocin; it was 98% identical with acidocin B produced by Lactobacillus acidophilus M46</description><subject>acidocin B</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>bacteriocin</subject><subject>BACTERIOCINAS</subject><subject>BACTERIOCINE</subject><subject>BACTERIOCINS</subject><subject>Bacteriocins - chemistry</subject><subject>Bacteriocins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gassericin A</subject><subject>GENE</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>GENES</subject><subject>HIDROFOBICIDAD</subject><subject>Humans</subject><subject>HYDROPHOBICITE</subject><subject>HYDROPHOBICITY</subject><subject>Infant</subject><subject>lactic acid bacteria</subject><subject>LACTOBACILLUS</subject><subject>Lactobacillus - metabolism</subject><subject>Lactobacillus gasseri</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>PEPTIDOS</subject><subject>Physiology and metabolism</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Analysis, DNA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkGPFCEQhYnRrOPqxbsJiRsPG3uEhqbhONmoq5lEE_VMgK6eYcM0I3TH9A_wf0tnZvfgQU8k9T4eVfVA6CUla1q39J21di3qtZTtI7SijLeVULx9jFZEUVFJ3tCn6FnOd4SUQkMv0IUSXNZUrdDvb_BzgsEBNoMJc_YZ2xm7EAc_7HDs8bgHnMc0uXFKJuAdDLCUdyZnSN75AW_eYoP3c5ficR-td9gaNxYtLuIxxW5y0C2u21KPRfQhTPneAW83TD1HT3oTMrw4n5fox4f3329uq-2Xj59uNtvKNYKPFTimJGcCGG2pU7yrFWdWWk4bDmCd5T3UxEigQraC90qBIpQIQg0B4DW7RG9OvqWtMnYe9cFnByGYAeKUdatUS5kk_wVp2V-xpQV8_Rd4F6dUVlkYzhWvhaKyUNcnyqWYc4JeH5M_mDRrSvQSoS4RalHrEmGBX50tJ3uA7gE9Z1b0q7NusjOhT2ZwPj9gNeNSqmXW5oT5oY_pYH7FFDo9mjnEdH-H_ev53kRtdqlgn79SpdTyewhhfwC9gL51</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Kawai, Y. (Tohoku Univ., Sendai (Japan))</creator><creator>Saito, T</creator><creator>Suzuki, M</creator><creator>Itoh, T</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Sequence analysis by cloning of the structural gene of gassericin A, a hydrophobic bacteriocin produced by Lactobacillus gasseri LA39</title><author>Kawai, Y. (Tohoku Univ., Sendai (Japan)) ; Saito, T ; Suzuki, M ; Itoh, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c564t-ec398436e3171c94d2943b8b4154eebcb4fe20a8e168764f99e9010601a0ee423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>acidocin B</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>bacteriocin</topic><topic>BACTERIOCINAS</topic><topic>BACTERIOCINE</topic><topic>BACTERIOCINS</topic><topic>Bacteriocins - chemistry</topic><topic>Bacteriocins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gassericin A</topic><topic>GENE</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>GENES</topic><topic>HIDROFOBICIDAD</topic><topic>Humans</topic><topic>HYDROPHOBICITE</topic><topic>HYDROPHOBICITY</topic><topic>Infant</topic><topic>lactic acid bacteria</topic><topic>LACTOBACILLUS</topic><topic>Lactobacillus - metabolism</topic><topic>Lactobacillus gasseri</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>PEPTIDOS</topic><topic>Physiology and metabolism</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Analysis, DNA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawai, Y. (Tohoku Univ., Sendai (Japan))</creatorcontrib><creatorcontrib>Saito, T</creatorcontrib><creatorcontrib>Suzuki, M</creatorcontrib><creatorcontrib>Itoh, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawai, Y. (Tohoku Univ., Sendai (Japan))</au><au>Saito, T</au><au>Suzuki, M</au><au>Itoh, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence analysis by cloning of the structural gene of gassericin A, a hydrophobic bacteriocin produced by Lactobacillus gasseri LA39</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>62</volume><issue>5</issue><spage>887</spage><epage>892</epage><pages>887-892</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Gassericin A, a bacteriocin from Lactobacillus gasseri LA39, was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis,unlike the estimated 3,800 found by SDS-PAGE. However, when the purified preparation was treated with lysylendopeptidase, it migrated as a single band to 5,600 with bacteriocin activity on SDS-PAGE. N- and C-terminal amino acids could not be identified. The internal amino acid could be identified after gassericin A was hydrolyzed with lysylendopeptidase. The DNA of the structural gene of gassericin A was sequenced by cloning of the gene from chromosomal DNA with an oligonucleotide probe. The structural gene of gassericin A was found on the chromosomal DNA as an open reading frame encoding a protein composed of 91 amino acids. The amino acid sequence of mature gassericin A was predicted to be 58 residues from the DNA sequence and results of mass analysis. These results suggested that gassericin A has a closed circular structure with N- and C-terminals linked. Gassericin A is a hydrophobic class II bacteriocin; it was 98% identical with acidocin B produced by Lactobacillus acidophilus M46</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>9648219</pmid><doi>10.1271/bbb.62.887</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1998-05, Vol.62 (5), p.887-892 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_2348892 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | acidocin B Amino Acid Sequence Animals bacteriocin BACTERIOCINAS BACTERIOCINE BACTERIOCINS Bacteriocins - chemistry Bacteriocins - genetics Bacteriology Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CLONACION CLONAGE CLONING Cloning, Molecular DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology gassericin A GENE Gene Expression Regulation, Bacterial GENES HIDROFOBICIDAD Humans HYDROPHOBICITE HYDROPHOBICITY Infant lactic acid bacteria LACTOBACILLUS Lactobacillus - metabolism Lactobacillus gasseri Microbiology Mission oriented research Molecular Sequence Data NUCLEOTIDE SEQUENCE Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains PEPTIDE PEPTIDES PEPTIDOS Physiology and metabolism PURIFICACION PURIFICATION SECUENCIA NUCLEOTIDICA Sequence Analysis, DNA SEQUENCE NUCLEOTIDIQUE |
| title | Sequence analysis by cloning of the structural gene of gassericin A, a hydrophobic bacteriocin produced by Lactobacillus gasseri LA39 |
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