Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38
Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was const...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (5), p.992-999 |
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| creator | Washio, K., Hokkaido Univ., Sapporo (Japan) Lim. S.P Roongsawang, N Morikawa, M |
| description | Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology. |
| doi_str_mv | 10.1271/bbb.90860 |
| format | Article |
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MIS38</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>Open Access Titles of Japan</source><source>Free Full-Text Journals in Chemistry</source><creator>Washio, K., Hokkaido Univ., Sapporo (Japan) ; Lim. S.P ; Roongsawang, N ; Morikawa, M</creator><creatorcontrib>Washio, K., Hokkaido Univ., Sapporo (Japan) ; Lim. S.P ; Roongsawang, N ; Morikawa, M</creatorcontrib><description>Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.90860</identifier><identifier>PMID: 20460722</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>(p)ppGpp ; arthrofactin ; Biological and medical sciences ; BIOLOGICAL CONTAMINATION ; CONSTRUCCION ; CONSTRUCTION ; CONTAMINACION BIOLOGICA ; CONTAMINATION BIOLOGIQUE ; DNA Transposable Elements - genetics ; Epistasis, Genetic - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial - genetics ; Guanosine Tetraphosphate - biosynthesis ; HIDROLASAS ; http://www.fao.org/aos/agrovoc#c_28318 ; http://www.fao.org/aos/agrovoc#c_35060 ; http://www.fao.org/aos/agrovoc#c_3729 ; http://www.fao.org/aos/agrovoc#c_37466 ; http://www.fao.org/aos/agrovoc#c_4320 ; http://www.fao.org/aos/agrovoc#c_49903 ; http://www.fao.org/aos/agrovoc#c_5013 ; http://www.fao.org/aos/agrovoc#c_6200 ; http://www.fao.org/aos/agrovoc#c_6304 ; http://www.fao.org/aos/agrovoc#c_7529 ; HYDROLASE ; HYDROLASES ; LIGASAS ; LIGASE ; LIGASES ; Lipopeptides - biosynthesis ; Lipopeptides - deficiency ; MECANISMOS DE ACCION ; MODE D'ACTION ; MODE OF ACTION ; MORFOLOGIA ; MORPHOLOGIE ; MORPHOLOGY ; Mutagenesis ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; nonribosomal peptide synthetase ; Peptides, Cyclic - biosynthesis ; Peptides, Cyclic - deficiency ; PRODUCCION ; PRODUCTION ; PSEUDOMONAS ; Pseudomonas - genetics ; Pseudomonas - metabolism ; Pseudomonas sp. MIS38 ; spoT ; SURFACTANT ; SURFACTANTES ; SURFACTANTS</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2010, Vol.74 (5), p.992-999</ispartof><rights>2010 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c634t-c228d05e2b4e31ccf8fd41a9415c854b26446f51260b5cce671950a05fa1e7973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23147147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20460722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Washio, K., Hokkaido Univ., Sapporo (Japan)</creatorcontrib><creatorcontrib>Lim. S.P</creatorcontrib><creatorcontrib>Roongsawang, N</creatorcontrib><creatorcontrib>Morikawa, M</creatorcontrib><title>Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology.</description><subject>(p)ppGpp</subject><subject>arthrofactin</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL CONTAMINATION</subject><subject>CONSTRUCCION</subject><subject>CONSTRUCTION</subject><subject>CONTAMINACION BIOLOGICA</subject><subject>CONTAMINATION BIOLOGIQUE</subject><subject>DNA Transposable Elements - genetics</subject><subject>Epistasis, Genetic - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial - genetics</subject><subject>Guanosine Tetraphosphate - biosynthesis</subject><subject>HIDROLASAS</subject><subject>http://www.fao.org/aos/agrovoc#c_28318</subject><subject>http://www.fao.org/aos/agrovoc#c_35060</subject><subject>http://www.fao.org/aos/agrovoc#c_3729</subject><subject>http://www.fao.org/aos/agrovoc#c_37466</subject><subject>http://www.fao.org/aos/agrovoc#c_4320</subject><subject>http://www.fao.org/aos/agrovoc#c_49903</subject><subject>http://www.fao.org/aos/agrovoc#c_5013</subject><subject>http://www.fao.org/aos/agrovoc#c_6200</subject><subject>http://www.fao.org/aos/agrovoc#c_6304</subject><subject>http://www.fao.org/aos/agrovoc#c_7529</subject><subject>HYDROLASE</subject><subject>HYDROLASES</subject><subject>LIGASAS</subject><subject>LIGASE</subject><subject>LIGASES</subject><subject>Lipopeptides - biosynthesis</subject><subject>Lipopeptides - deficiency</subject><subject>MECANISMOS DE ACCION</subject><subject>MODE D'ACTION</subject><subject>MODE OF ACTION</subject><subject>MORFOLOGIA</subject><subject>MORPHOLOGIE</subject><subject>MORPHOLOGY</subject><subject>Mutagenesis</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>nonribosomal peptide synthetase</subject><subject>Peptides, Cyclic - biosynthesis</subject><subject>Peptides, Cyclic - deficiency</subject><subject>PRODUCCION</subject><subject>PRODUCTION</subject><subject>PSEUDOMONAS</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - metabolism</subject><subject>Pseudomonas sp. MIS38</subject><subject>spoT</subject><subject>SURFACTANT</subject><subject>SURFACTANTES</subject><subject>SURFACTANTS</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt-K1DAUh4Mo7jh64QMoARHxomNOkibppSz-GVlxQb0uaZrsZOkk3aRFxpfwlc1uZ1VEEA4ETr7zS8IXhB4D2QCV8Krruk1DlCB30AoYl5VouLyLVqQBUSlewwl6kPMlIaVRw310QgkXRFK6Qj-2vQ2Td97oyceAdeix2emkzWST_740o8PTzuILG2zGyeYxhuy7wWIX083OmGI_mz9ZczCDN3jwYxztOPneYp2mXYquJPuAuwM-z3bu4z4GnXEeN_jj9jNTD9E9p4dsHx3XNfr69s2X0_fV2ad329PXZ5URjE-VoVT1pLa045aBMU65noNuONRG1byjgnPhaqCCdLUxVkhoaqJJ7TRY2Ui2Ri-W3HL1q9nmqd37bOww6GDjnFupJEjVCPp_kjEgisF15rO_yMs4p1Ce0QLnjeKclVqjlwtlUsw5WdeOye91OrRA2mudbdHZ3ugs7NNj4tztbf-LvPVXgOdHQGejB5d0MD7_5hhwWapwfOF8KM72-ltMQ99O-jDEdDvE_nX-k2XM6djqi1SoD-eUQPlJDECwnzVAwtA</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Washio, K., Hokkaido Univ., Sapporo (Japan)</creator><creator>Lim. S.P</creator><creator>Roongsawang, N</creator><creator>Morikawa, M</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2010</creationdate><title>Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38</title><author>Washio, K., Hokkaido Univ., Sapporo (Japan) ; Lim. S.P ; Roongsawang, N ; Morikawa, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c634t-c228d05e2b4e31ccf8fd41a9415c854b26446f51260b5cce671950a05fa1e7973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>(p)ppGpp</topic><topic>arthrofactin</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL CONTAMINATION</topic><topic>CONSTRUCCION</topic><topic>CONSTRUCTION</topic><topic>CONTAMINACION BIOLOGICA</topic><topic>CONTAMINATION BIOLOGIQUE</topic><topic>DNA Transposable Elements - genetics</topic><topic>Epistasis, Genetic - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial - genetics</topic><topic>Guanosine Tetraphosphate - biosynthesis</topic><topic>HIDROLASAS</topic><topic>http://www.fao.org/aos/agrovoc#c_28318</topic><topic>http://www.fao.org/aos/agrovoc#c_35060</topic><topic>http://www.fao.org/aos/agrovoc#c_3729</topic><topic>http://www.fao.org/aos/agrovoc#c_37466</topic><topic>http://www.fao.org/aos/agrovoc#c_4320</topic><topic>http://www.fao.org/aos/agrovoc#c_49903</topic><topic>http://www.fao.org/aos/agrovoc#c_5013</topic><topic>http://www.fao.org/aos/agrovoc#c_6200</topic><topic>http://www.fao.org/aos/agrovoc#c_6304</topic><topic>http://www.fao.org/aos/agrovoc#c_7529</topic><topic>HYDROLASE</topic><topic>HYDROLASES</topic><topic>LIGASAS</topic><topic>LIGASE</topic><topic>LIGASES</topic><topic>Lipopeptides - biosynthesis</topic><topic>Lipopeptides - deficiency</topic><topic>MECANISMOS DE ACCION</topic><topic>MODE D'ACTION</topic><topic>MODE OF ACTION</topic><topic>MORFOLOGIA</topic><topic>MORPHOLOGIE</topic><topic>MORPHOLOGY</topic><topic>Mutagenesis</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>nonribosomal peptide synthetase</topic><topic>Peptides, Cyclic - biosynthesis</topic><topic>Peptides, Cyclic - deficiency</topic><topic>PRODUCCION</topic><topic>PRODUCTION</topic><topic>PSEUDOMONAS</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - metabolism</topic><topic>Pseudomonas sp. 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S.P</au><au>Roongsawang, N</au><au>Morikawa, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2010</date><risdate>2010</risdate><volume>74</volume><issue>5</issue><spage>992</spage><epage>999</epage><pages>992-999</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>20460722</pmid><doi>10.1271/bbb.90860</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2010, Vol.74 (5), p.992-999 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_23147147 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | (p)ppGpp arthrofactin Biological and medical sciences BIOLOGICAL CONTAMINATION CONSTRUCCION CONSTRUCTION CONTAMINACION BIOLOGICA CONTAMINATION BIOLOGIQUE DNA Transposable Elements - genetics Epistasis, Genetic - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial - genetics Guanosine Tetraphosphate - biosynthesis HIDROLASAS http://www.fao.org/aos/agrovoc#c_28318 http://www.fao.org/aos/agrovoc#c_35060 http://www.fao.org/aos/agrovoc#c_3729 http://www.fao.org/aos/agrovoc#c_37466 http://www.fao.org/aos/agrovoc#c_4320 http://www.fao.org/aos/agrovoc#c_49903 http://www.fao.org/aos/agrovoc#c_5013 http://www.fao.org/aos/agrovoc#c_6200 http://www.fao.org/aos/agrovoc#c_6304 http://www.fao.org/aos/agrovoc#c_7529 HYDROLASE HYDROLASES LIGASAS LIGASE LIGASES Lipopeptides - biosynthesis Lipopeptides - deficiency MECANISMOS DE ACCION MODE D'ACTION MODE OF ACTION MORFOLOGIA MORPHOLOGIE MORPHOLOGY Mutagenesis MUTANT MUTANTES MUTANTS Mutation nonribosomal peptide synthetase Peptides, Cyclic - biosynthesis Peptides, Cyclic - deficiency PRODUCCION PRODUCTION PSEUDOMONAS Pseudomonas - genetics Pseudomonas - metabolism Pseudomonas sp. MIS38 spoT SURFACTANT SURFACTANTES SURFACTANTS |
| title | Identification and characterization of the genes responsible for the production of the cyclic lipopeptide arthrofactin by Pseudomonas sp. MIS38 |
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