Density gradients in packed columns : I. Effects of density gradients on retention and separation speed
Density gradients in packed capillary columns operating under the extreme pressure drops typical for solvating gas chromatography were investigated by on-column spectroscopic measurements and compared to a theoretical model. Laser-induced fluorescence was used to follow the elution of various analyt...
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| Veröffentlicht in: | Journal of chromatography 2009-07, Vol.1216 (29), p.5588-5593 |
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| container_end_page | 5593 |
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| container_issue | 29 |
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| container_title | Journal of chromatography |
| container_volume | 1216 |
| creator | Baker, L. Robert Stark, Marisa A Orton, Andrew W Horn, Brent A Goates, Steven R |
| description | Density gradients in packed capillary columns operating under the extreme pressure drops typical for solvating gas chromatography were investigated by on-column spectroscopic measurements and compared to a theoretical model. Laser-induced fluorescence was used to follow the elution of various analytes, and Raman spectroscopy was used to measure the density of the mobile phase, each with respect to column position. Mobile phase linear velocity initially increases gradually, and then rises rapidly near the column outlet. High flow rates near the column outlet are offset by a loss of mobile phase solvating power which ultimately limits the speed of separation. These results represent an extreme case for illuminating factors affecting supercritical fluid separation techniques in general. |
| doi_str_mv | 10.1016/j.chroma.2009.05.042 |
| format | Article |
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Robert</creatorcontrib><creatorcontrib>Stark, Marisa A</creatorcontrib><creatorcontrib>Orton, Andrew W</creatorcontrib><creatorcontrib>Horn, Brent A</creatorcontrib><creatorcontrib>Goates, Steven R</creatorcontrib><title>Density gradients in packed columns : I. Effects of density gradients on retention and separation speed</title><title>Journal of chromatography</title><addtitle>J Chromatogr A</addtitle><description>Density gradients in packed capillary columns operating under the extreme pressure drops typical for solvating gas chromatography were investigated by on-column spectroscopic measurements and compared to a theoretical model. Laser-induced fluorescence was used to follow the elution of various analytes, and Raman spectroscopy was used to measure the density of the mobile phase, each with respect to column position. Mobile phase linear velocity initially increases gradually, and then rises rapidly near the column outlet. 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These results represent an extreme case for illuminating factors affecting supercritical fluid separation techniques in general.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Gas chromatographic methods</subject><subject>Other chromatographic methods</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNplkE1PAjEQhhujEUT_gdFePO46bXe3rTeDqCQkHpQzKe0UF9mPtHDg39sIevE0z2SemWReQq4Z5AxYdb_O7WfoGpNzAJ1DmUPBT8iQKSkyIaU6JUMAzjJdSTEgFzGuAZgEyc_JgOlCy1LBkKyesI31dk9Xwbga222kdUt7Y7_QUdttdk0b6QOd5nTiPdo07jx1_3a6lgbcJqwTmdbRiL0J5qeNPaK7JGfebCJeHeuIzJ8nH-PXbPb2Mh0_zjLPK7HNClZpvbRcO_RclU4oLQqlrGVYofPC-YKhM4VEhkYBovaoUScwqgRbiBG5Odztd8sG3aIPdWPCfvH7cRLujoKJ1mx8MK2t45_HWSUV5yJ5twfPm25hViE583cOTKTsC8mAi29OeHH9</recordid><startdate>20090717</startdate><enddate>20090717</enddate><creator>Baker, L. 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Robert</creatorcontrib><creatorcontrib>Stark, Marisa A</creatorcontrib><creatorcontrib>Orton, Andrew W</creatorcontrib><creatorcontrib>Horn, Brent A</creatorcontrib><creatorcontrib>Goates, Steven R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><jtitle>Journal of chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baker, L. Robert</au><au>Stark, Marisa A</au><au>Orton, Andrew W</au><au>Horn, Brent A</au><au>Goates, Steven R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Density gradients in packed columns : I. Effects of density gradients on retention and separation speed</atitle><jtitle>Journal of chromatography</jtitle><addtitle>J Chromatogr A</addtitle><date>2009-07-17</date><risdate>2009</risdate><volume>1216</volume><issue>29</issue><spage>5588</spage><epage>5593</epage><pages>5588-5593</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><coden>JOCRAM</coden><abstract>Density gradients in packed capillary columns operating under the extreme pressure drops typical for solvating gas chromatography were investigated by on-column spectroscopic measurements and compared to a theoretical model. Laser-induced fluorescence was used to follow the elution of various analytes, and Raman spectroscopy was used to measure the density of the mobile phase, each with respect to column position. Mobile phase linear velocity initially increases gradually, and then rises rapidly near the column outlet. 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| ispartof | Journal of chromatography, 2009-07, Vol.1216 (29), p.5588-5593 |
| issn | 0021-9673 1873-3778 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_21678223 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Gas chromatographic methods Other chromatographic methods |
| title | Density gradients in packed columns : I. Effects of density gradients on retention and separation speed |
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