Platelet-derived microparticles and coagulation activation in breast cancer patients
Summary In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patien...
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description | Summary
In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patients and 13 women with benign breast tumors were included in the study. Microparticles (MP) were examined by electron microscopy and FACS analysis using labels for annexinV (total numbers), CD61 (PMP), CD62P and CD63 (activated platelets), CD62E (endothelial cells), CD45 (leukocytes) as well as CD142 (tissue factor). Prothrombin fragment 1+2 (F1+2) and thrombin generation were measured as blood coagulation markers. Numbers of annexin V+-MP were highest in breast cancer patients with larger tumor size (T2; median = 5,637×10
6
/l; range = 2,852–8,613) and patients with distant metastases (M1; median = 6,102×10
6
/l; range = 3,350–7,445), and differed significantly from patients with insitu tumor (Tis; median = 3,220×10
6
/l; range = 2,277–4,124; p = 0.019), small tumor size (T1; median = 3,281×10
6
/l; range 2,356–4,861; p = 0.043) and women with benign breast tumor (median = 4,108×10
6
/l; range = 2,530–4,874; p = 0.040). A total of 82.3% of MP were from platelets,14.6 % from endothelial cells and 0.3% from leukocytes. Less than 10% of PMP showed degranulation markers. Larger tumor size (T2) and metastases correlated with high counts of PMP and with highest F1+2 levels. Since prothrombin levels and thrombin generation did not parallel MP levels, we speculate that MP act in the microenvironment of tumor tissue and may thus not be an exclusive parameter reflecting in-vivo procoagulant activity. |
doi_str_mv | 10.1160/TH07-10-0602 |
format | Article |
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In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patients and 13 women with benign breast tumors were included in the study. Microparticles (MP) were examined by electron microscopy and FACS analysis using labels for annexinV (total numbers), CD61 (PMP), CD62P and CD63 (activated platelets), CD62E (endothelial cells), CD45 (leukocytes) as well as CD142 (tissue factor). Prothrombin fragment 1+2 (F1+2) and thrombin generation were measured as blood coagulation markers. Numbers of annexin V+-MP were highest in breast cancer patients with larger tumor size (T2; median = 5,637×10
6
/l; range = 2,852–8,613) and patients with distant metastases (M1; median = 6,102×10
6
/l; range = 3,350–7,445), and differed significantly from patients with insitu tumor (Tis; median = 3,220×10
6
/l; range = 2,277–4,124; p = 0.019), small tumor size (T1; median = 3,281×10
6
/l; range 2,356–4,861; p = 0.043) and women with benign breast tumor (median = 4,108×10
6
/l; range = 2,530–4,874; p = 0.040). A total of 82.3% of MP were from platelets,14.6 % from endothelial cells and 0.3% from leukocytes. Less than 10% of PMP showed degranulation markers. Larger tumor size (T2) and metastases correlated with high counts of PMP and with highest F1+2 levels. Since prothrombin levels and thrombin generation did not parallel MP levels, we speculate that MP act in the microenvironment of tumor tissue and may thus not be an exclusive parameter reflecting in-vivo procoagulant activity.</description><identifier>ISSN: 0340-6245</identifier><identifier>EISSN: 2567-689X</identifier><identifier>DOI: 10.1160/TH07-10-0602</identifier><identifier>PMID: 18841290</identifier><identifier>CODEN: THHADQ</identifier><language>eng</language><publisher>Stuttgart: Schattauer Verlag für Medizin und Naturwissenschaften</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Blood Coagulation - physiology ; Blood coagulation. Blood cells ; Blood Platelets - pathology ; Blood Platelets - ultrastructure ; breast cancer ; Breast Neoplasms - complications ; Case-Control Studies ; Cellular Proteolysis and Oncology ; electron microscopy ; Endothelial Cells - pathology ; Endothelial Cells - ultrastructure ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Hematologic and hematopoietic diseases ; Humans ; Leukocytes - pathology ; Leukocytes - ultrastructure ; Medical sciences ; Microscopy, Electron ; Middle Aged ; Molecular and cellular biology ; Neoplasms - complications ; Particle Size ; Peptide Fragments - metabolism ; Platelet diseases and coagulopathies ; Platelet-derived microparticles ; Prospective Studies ; Prothrombin - metabolism ; prothrombin fragment 1+2 ; Thrombin - metabolism ; thrombin generation ; Thrombosis - complications ; Thrombosis - pathology</subject><ispartof>Thrombosis and haemostasis, 2008-10, Vol.100 (4), p.663-669</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-1004641d876a91652795adc9b409298989feee0743a3d7e578ac1099db61915a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1160/TH07-10-0602.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1160/TH07-10-0602$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,780,784,3016,27923,27924,54558,54559</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20707234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18841290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toth, Bettina</creatorcontrib><creatorcontrib>Liebhardt, Susanne</creatorcontrib><creatorcontrib>Steinig, Kerstin</creatorcontrib><creatorcontrib>Ditsch, Nina</creatorcontrib><creatorcontrib>Rank, Andreas</creatorcontrib><creatorcontrib>Bauerfeind, Ingo</creatorcontrib><creatorcontrib>Spannagl, Michael</creatorcontrib><creatorcontrib>Friese, Klaus</creatorcontrib><creatorcontrib>Reininger, Armin J.</creatorcontrib><title>Platelet-derived microparticles and coagulation activation in breast cancer patients</title><title>Thrombosis and haemostasis</title><addtitle>Thromb Haemost</addtitle><description>Summary
In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patients and 13 women with benign breast tumors were included in the study. Microparticles (MP) were examined by electron microscopy and FACS analysis using labels for annexinV (total numbers), CD61 (PMP), CD62P and CD63 (activated platelets), CD62E (endothelial cells), CD45 (leukocytes) as well as CD142 (tissue factor). Prothrombin fragment 1+2 (F1+2) and thrombin generation were measured as blood coagulation markers. Numbers of annexin V+-MP were highest in breast cancer patients with larger tumor size (T2; median = 5,637×10
6
/l; range = 2,852–8,613) and patients with distant metastases (M1; median = 6,102×10
6
/l; range = 3,350–7,445), and differed significantly from patients with insitu tumor (Tis; median = 3,220×10
6
/l; range = 2,277–4,124; p = 0.019), small tumor size (T1; median = 3,281×10
6
/l; range 2,356–4,861; p = 0.043) and women with benign breast tumor (median = 4,108×10
6
/l; range = 2,530–4,874; p = 0.040). A total of 82.3% of MP were from platelets,14.6 % from endothelial cells and 0.3% from leukocytes. Less than 10% of PMP showed degranulation markers. Larger tumor size (T2) and metastases correlated with high counts of PMP and with highest F1+2 levels. Since prothrombin levels and thrombin generation did not parallel MP levels, we speculate that MP act in the microenvironment of tumor tissue and may thus not be an exclusive parameter reflecting in-vivo procoagulant activity.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Blood Coagulation - physiology</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - pathology</subject><subject>Blood Platelets - ultrastructure</subject><subject>breast cancer</subject><subject>Breast Neoplasms - complications</subject><subject>Case-Control Studies</subject><subject>Cellular Proteolysis and Oncology</subject><subject>electron microscopy</subject><subject>Endothelial Cells - pathology</subject><subject>Endothelial Cells - ultrastructure</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukocytes - pathology</subject><subject>Leukocytes - ultrastructure</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Molecular and cellular biology</subject><subject>Neoplasms - complications</subject><subject>Particle Size</subject><subject>Peptide Fragments - metabolism</subject><subject>Platelet diseases and coagulopathies</subject><subject>Platelet-derived microparticles</subject><subject>Prospective Studies</subject><subject>Prothrombin - metabolism</subject><subject>prothrombin fragment 1+2</subject><subject>Thrombin - metabolism</subject><subject>thrombin generation</subject><subject>Thrombosis - complications</subject><subject>Thrombosis - pathology</subject><issn>0340-6245</issn><issn>2567-689X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNq9kc-L1TAQx4Mo7tvVm2fpRS9rNemPpDkui7rCgh6e4C1M03k2j7apmXTF_96UPlZBvInkkEz4MJ_MN4w9E_y1EJK_2d9wlQuec8mLB2xX1FLlstFfHrIdLyuey6Kqz9g50ZFzIStdP2ZnomkqUWi-Y_tPA0QcMOYdBneHXTY6G_wMITo7IGUwdZn18HVJnPNTBja6u-3opqwNCBQzC5PFkM3pHqdIT9ijAwyET0_7Bfv87u3--ia__fj-w_XVbW5lzWN6NK9kJbpGSdBC1oXSNXRWtxXXhW7SOiAiV1UJZaewVg1YwbXuWim0qKG8YC-3vnPw3xakaEZHFocBJvQLGanTvCUXCXy1gWk0ooAHMwc3QvhhBDdrimZNcS3WFBP-_NR3aUfsfsGn2BLw4gQAWRgOIc3v6J4ruOKqKKvEXW5c7B2OaI5-CVNK5G_a40aT7SFGWDDct4x98GPrKVnSj5gecPQUYa2tn-KauoFg-_SFxhEtaFShzAjTQja4OSZJUTeGev_d9HEckqz7hzKa0ToY_hAmzfQfNL_N9ROX6QUW</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Toth, Bettina</creator><creator>Liebhardt, Susanne</creator><creator>Steinig, Kerstin</creator><creator>Ditsch, Nina</creator><creator>Rank, Andreas</creator><creator>Bauerfeind, Ingo</creator><creator>Spannagl, Michael</creator><creator>Friese, Klaus</creator><creator>Reininger, Armin J.</creator><general>Schattauer Verlag für Medizin und Naturwissenschaften</general><general>Schattauer GmbH</general><general>Schattauer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Platelet-derived microparticles and coagulation activation in breast cancer patients</title><author>Toth, Bettina ; Liebhardt, Susanne ; Steinig, Kerstin ; Ditsch, Nina ; Rank, Andreas ; Bauerfeind, Ingo ; Spannagl, Michael ; Friese, Klaus ; Reininger, Armin J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-1004641d876a91652795adc9b409298989feee0743a3d7e578ac1099db61915a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Blood Coagulation - physiology</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - pathology</topic><topic>Blood Platelets - ultrastructure</topic><topic>breast cancer</topic><topic>Breast Neoplasms - complications</topic><topic>Case-Control Studies</topic><topic>Cellular Proteolysis and Oncology</topic><topic>electron microscopy</topic><topic>Endothelial Cells - pathology</topic><topic>Endothelial Cells - ultrastructure</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukocytes - pathology</topic><topic>Leukocytes - ultrastructure</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Molecular and cellular biology</topic><topic>Neoplasms - complications</topic><topic>Particle Size</topic><topic>Peptide Fragments - metabolism</topic><topic>Platelet diseases and coagulopathies</topic><topic>Platelet-derived microparticles</topic><topic>Prospective Studies</topic><topic>Prothrombin - metabolism</topic><topic>prothrombin fragment 1+2</topic><topic>Thrombin - metabolism</topic><topic>thrombin generation</topic><topic>Thrombosis - complications</topic><topic>Thrombosis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toth, Bettina</creatorcontrib><creatorcontrib>Liebhardt, Susanne</creatorcontrib><creatorcontrib>Steinig, Kerstin</creatorcontrib><creatorcontrib>Ditsch, Nina</creatorcontrib><creatorcontrib>Rank, Andreas</creatorcontrib><creatorcontrib>Bauerfeind, Ingo</creatorcontrib><creatorcontrib>Spannagl, Michael</creatorcontrib><creatorcontrib>Friese, Klaus</creatorcontrib><creatorcontrib>Reininger, Armin J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis and haemostasis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toth, Bettina</au><au>Liebhardt, Susanne</au><au>Steinig, Kerstin</au><au>Ditsch, Nina</au><au>Rank, Andreas</au><au>Bauerfeind, Ingo</au><au>Spannagl, Michael</au><au>Friese, Klaus</au><au>Reininger, Armin J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet-derived microparticles and coagulation activation in breast cancer patients</atitle><jtitle>Thrombosis and haemostasis</jtitle><addtitle>Thromb Haemost</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>100</volume><issue>4</issue><spage>663</spage><epage>669</epage><pages>663-669</pages><issn>0340-6245</issn><eissn>2567-689X</eissn><coden>THHADQ</coden><abstract>Summary
In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patients and 13 women with benign breast tumors were included in the study. Microparticles (MP) were examined by electron microscopy and FACS analysis using labels for annexinV (total numbers), CD61 (PMP), CD62P and CD63 (activated platelets), CD62E (endothelial cells), CD45 (leukocytes) as well as CD142 (tissue factor). Prothrombin fragment 1+2 (F1+2) and thrombin generation were measured as blood coagulation markers. Numbers of annexin V+-MP were highest in breast cancer patients with larger tumor size (T2; median = 5,637×10
6
/l; range = 2,852–8,613) and patients with distant metastases (M1; median = 6,102×10
6
/l; range = 3,350–7,445), and differed significantly from patients with insitu tumor (Tis; median = 3,220×10
6
/l; range = 2,277–4,124; p = 0.019), small tumor size (T1; median = 3,281×10
6
/l; range 2,356–4,861; p = 0.043) and women with benign breast tumor (median = 4,108×10
6
/l; range = 2,530–4,874; p = 0.040). A total of 82.3% of MP were from platelets,14.6 % from endothelial cells and 0.3% from leukocytes. Less than 10% of PMP showed degranulation markers. Larger tumor size (T2) and metastases correlated with high counts of PMP and with highest F1+2 levels. Since prothrombin levels and thrombin generation did not parallel MP levels, we speculate that MP act in the microenvironment of tumor tissue and may thus not be an exclusive parameter reflecting in-vivo procoagulant activity.</abstract><cop>Stuttgart</cop><pub>Schattauer Verlag für Medizin und Naturwissenschaften</pub><pmid>18841290</pmid><doi>10.1160/TH07-10-0602</doi><tpages>7</tpages></addata></record> |
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subjects | Adult Aged Aged, 80 and over Biological and medical sciences Blood Coagulation - physiology Blood coagulation. Blood cells Blood Platelets - pathology Blood Platelets - ultrastructure breast cancer Breast Neoplasms - complications Case-Control Studies Cellular Proteolysis and Oncology electron microscopy Endothelial Cells - pathology Endothelial Cells - ultrastructure Female Flow Cytometry Fundamental and applied biological sciences. Psychology Hematologic and hematopoietic diseases Humans Leukocytes - pathology Leukocytes - ultrastructure Medical sciences Microscopy, Electron Middle Aged Molecular and cellular biology Neoplasms - complications Particle Size Peptide Fragments - metabolism Platelet diseases and coagulopathies Platelet-derived microparticles Prospective Studies Prothrombin - metabolism prothrombin fragment 1+2 Thrombin - metabolism thrombin generation Thrombosis - complications Thrombosis - pathology |
title | Platelet-derived microparticles and coagulation activation in breast cancer patients |
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