Optimal Probe Length and Target Location for Electrochemical Detection of Selected Uropathogens at Ambient Temperature

We have previously demonstrated the clinical validity of the rapid detection of uropathogens by use of a DNA biosensor. This assay involves the hybridization of capture and detector probe pairs with bacterial 16S rRNA target molecules to form a DNA-RNA sandwich on the sensor surface. Horseradish per...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-08, Vol.46 (8), p.2707-2716
Hauptverfasser:	Mastali, Mitra, Babbitt, Jane T, Li, Yang, Landaw, Elliot M, Gau, Vincent, Churchill, Bernard M, Haake, David A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Base Sequence Biological and medical sciences Biosensing Techniques - methods Fundamental and applied biological sciences. Psychology Gram-Negative Bacteria - classification Gram-Negative Bacteria - isolation & purification Gram-Negative Bacterial Infections - diagnosis Humans Microbiology Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization - methods Oligonucleotide Probes - genetics Point-of-Care Systems RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Sequence Alignment Temperature Urinary Tract Infections - microbiology
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