Under-Expression of CD24 in Pre-Eclamptic Placental Tissues Determined by Quantitative Real-Time RT-PCR
Background: Pre-eclampsia is a pregnancy-related disorder present in about 5–7% of all pregnancies. CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues. Methods: We collected placental tissues from pre-eclamptic (n = 16...
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| Veröffentlicht in: | Fetal diagnosis and therapy 2008-01, Vol.23 (4), p.263-266 |
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| creator | Nagy, Bálint Berkes, Enikő Rigó, Barbara Bán, Zoltán Papp, Zoltán Hupuczi, Petronella |
| description | Background: Pre-eclampsia is a pregnancy-related disorder present in about 5–7% of all pregnancies. CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues. Methods: We collected placental tissues from pre-eclamptic (n = 16) and healthy pregnancies (n = 16). We used the quantitative real-time PCR method with a primer-probe system for determination of CD24 gene expression. Results: We measured CD24 concentrations of 18.94 ± 26.86 ng/µl in the pre-eclamptic and 53.85 ± 92.05 ng/µl in the healthy placental tissues (p = 0.03). Conclusions: The quantitative real-time PCR method is suitable to determine CD24 expression in placental tissues. We suppose the low expression of CD24 may cause the enhanced immune reaction and could play a role in the abnormal development of placenta in pre-eclampsia. |
| doi_str_mv | 10.1159/000123612 |
| format | Article |
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CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues. Methods: We collected placental tissues from pre-eclamptic (n = 16) and healthy pregnancies (n = 16). We used the quantitative real-time PCR method with a primer-probe system for determination of CD24 gene expression. Results: We measured CD24 concentrations of 18.94 ± 26.86 ng/µl in the pre-eclamptic and 53.85 ± 92.05 ng/µl in the healthy placental tissues (p = 0.03). Conclusions: The quantitative real-time PCR method is suitable to determine CD24 expression in placental tissues. We suppose the low expression of CD24 may cause the enhanced immune reaction and could play a role in the abnormal development of placenta in pre-eclampsia.</description><identifier>ISSN: 1015-3837</identifier><identifier>EISSN: 1421-9964</identifier><identifier>DOI: 10.1159/000123612</identifier><identifier>PMID: 18417991</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adult ; Biological and medical sciences ; Case-Control Studies ; CD24 Antigen - genetics ; Delivery. Postpartum. Lactation ; Diseases of mother, fetus and pregnancy ; Female ; Gene Expression ; General aspects ; Gynecology. Andrology. Obstetrics ; HELLP Syndrome - genetics ; HELLP Syndrome - immunology ; Humans ; Medical sciences ; Placenta - immunology ; Pre-Eclampsia - genetics ; Pre-Eclampsia - immunology ; Pregnancy ; Pregnancy. Fetus. 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CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues. Methods: We collected placental tissues from pre-eclamptic (n = 16) and healthy pregnancies (n = 16). We used the quantitative real-time PCR method with a primer-probe system for determination of CD24 gene expression. Results: We measured CD24 concentrations of 18.94 ± 26.86 ng/µl in the pre-eclamptic and 53.85 ± 92.05 ng/µl in the healthy placental tissues (p = 0.03). Conclusions: The quantitative real-time PCR method is suitable to determine CD24 expression in placental tissues. We suppose the low expression of CD24 may cause the enhanced immune reaction and could play a role in the abnormal development of placenta in pre-eclampsia.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>CD24 Antigen - genetics</subject><subject>Delivery. Postpartum. 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Postpartum. Lactation</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Female</topic><topic>Gene Expression</topic><topic>General aspects</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>HELLP Syndrome - genetics</topic><topic>HELLP Syndrome - immunology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Placenta - immunology</topic><topic>Pre-Eclampsia - genetics</topic><topic>Pre-Eclampsia - immunology</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagy, Bálint</creatorcontrib><creatorcontrib>Berkes, Enikő</creatorcontrib><creatorcontrib>Rigó, Barbara</creatorcontrib><creatorcontrib>Bán, Zoltán</creatorcontrib><creatorcontrib>Papp, Zoltán</creatorcontrib><creatorcontrib>Hupuczi, Petronella</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Fetal diagnosis and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagy, Bálint</au><au>Berkes, Enikő</au><au>Rigó, Barbara</au><au>Bán, Zoltán</au><au>Papp, Zoltán</au><au>Hupuczi, Petronella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Under-Expression of CD24 in Pre-Eclamptic Placental Tissues Determined by Quantitative Real-Time RT-PCR</atitle><jtitle>Fetal diagnosis and therapy</jtitle><addtitle>Fetal Diagn Ther</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>23</volume><issue>4</issue><spage>263</spage><epage>266</epage><pages>263-266</pages><issn>1015-3837</issn><eissn>1421-9964</eissn><abstract>Background: Pre-eclampsia is a pregnancy-related disorder present in about 5–7% of all pregnancies. CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues. Methods: We collected placental tissues from pre-eclamptic (n = 16) and healthy pregnancies (n = 16). We used the quantitative real-time PCR method with a primer-probe system for determination of CD24 gene expression. Results: We measured CD24 concentrations of 18.94 ± 26.86 ng/µl in the pre-eclamptic and 53.85 ± 92.05 ng/µl in the healthy placental tissues (p = 0.03). Conclusions: The quantitative real-time PCR method is suitable to determine CD24 expression in placental tissues. We suppose the low expression of CD24 may cause the enhanced immune reaction and could play a role in the abnormal development of placenta in pre-eclampsia.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>18417991</pmid><doi>10.1159/000123612</doi><tpages>4</tpages></addata></record> |
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| ispartof | Fetal diagnosis and therapy, 2008-01, Vol.23 (4), p.263-266 |
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| language | eng |
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| source | Karger Journal Archive Collection; MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Case-Control Studies CD24 Antigen - genetics Delivery. Postpartum. Lactation Diseases of mother, fetus and pregnancy Female Gene Expression General aspects Gynecology. Andrology. Obstetrics HELLP Syndrome - genetics HELLP Syndrome - immunology Humans Medical sciences Placenta - immunology Pre-Eclampsia - genetics Pre-Eclampsia - immunology Pregnancy Pregnancy. Fetus. Placenta Reverse Transcriptase Polymerase Chain Reaction RNA - genetics RNA - metabolism |
| title | Under-Expression of CD24 in Pre-Eclamptic Placental Tissues Determined by Quantitative Real-Time RT-PCR |
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