Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification
This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnet...
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| Veröffentlicht in: | Journal of microelectromechanical systems 2008-04, Vol.17 (2), p.288-301 |
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| creator | LIEN, Kang-Yi LIN, Wang-Ying LEE, Yu-Fang WANG, Chih-Hao LEI, Huan-Yao LEE, Gwo-Bin |
| description | This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator can also be used as microheating chambers to perform RT-PCR. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses or bacteria, the target virus and bacteria were recognized and further separated and purified from the biosamples by a magnetic field generated by the bioseparator. The target purified virus/bacteria was then lysed to release ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) for the subsequent RT-PCR processes. Experimental results showed that the target virus/bacteria was successfully separated and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA was automatically completed by utilizing the on-chip microheaters and the micro temperature sensor. The high mixing efficiency of the two-way s-shape pump and the rapid heating/cooling rate of the microheating chambers can significantly shorten the pretreatment and diagnosis processes. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast disease diagnosis. |
| doi_str_mv | 10.1109/JMEMS.2008.916295 |
| format | Article |
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In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator can also be used as microheating chambers to perform RT-PCR. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses or bacteria, the target virus and bacteria were recognized and further separated and purified from the biosamples by a magnetic field generated by the bioseparator. The target purified virus/bacteria was then lysed to release ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) for the subsequent RT-PCR processes. Experimental results showed that the target virus/bacteria was successfully separated and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA was automatically completed by utilizing the on-chip microheaters and the micro temperature sensor. The high mixing efficiency of the two-way s-shape pump and the rapid heating/cooling rate of the microheating chambers can significantly shorten the pretreatment and diagnosis processes. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast disease diagnosis.</description><identifier>ISSN: 1057-7157</identifier><identifier>EISSN: 1941-0158</identifier><identifier>DOI: 10.1109/JMEMS.2008.916295</identifier><identifier>CODEN: JMIYET</identifier><language>eng</language><publisher>New York, NY: IEEE</publisher><subject>Applied fluid mechanics ; Applied sciences ; Biological and medical sciences ; Biotechnology ; Deoxyribonucleic acid ; DNA ; Enrichment ; Exact sciences and technology ; Fluid dynamics ; Fluidics ; Fundamental and applied biological sciences. Psychology ; Fundamental areas of phenomenology (including applications) ; Genetic engineering ; Genetic technics ; In vitro gene amplification. Pcr technique ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; magnetic bead ; Magnetic fields ; Magnetic separation ; Mechanical engineering. Machine design ; Mechanical instruments, equipment and techniques ; Methods. Procedures. Technologies ; microelectromechanical systems (MEMS) ; Microfluidics ; Micromechanical devices and systems ; Microorganisms ; Micropumps ; Physics ; Polymers ; Precision engineering, watch making ; pretreatment ; reverse-transcription polymerase chain reaction (RT-PCR) ; RNA ; Studies ; Target recognition ; Viruses ; Viruses (medical)</subject><ispartof>Journal of microelectromechanical systems, 2008-04, Vol.17 (2), p.288-301</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright The Institute of Electrical and Electronics Engineers, Inc. (IEEE) 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-c237721d0c87da76f0cff21034b826674c0fdb996da9f57419ecd26045c3ed803</citedby><cites>FETCH-LOGICAL-c481t-c237721d0c87da76f0cff21034b826674c0fdb996da9f57419ecd26045c3ed803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/4459701$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,776,780,792,27901,27902,54733</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/4459701$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20278034$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>LIEN, Kang-Yi</creatorcontrib><creatorcontrib>LIN, Wang-Ying</creatorcontrib><creatorcontrib>LEE, Yu-Fang</creatorcontrib><creatorcontrib>WANG, Chih-Hao</creatorcontrib><creatorcontrib>LEI, Huan-Yao</creatorcontrib><creatorcontrib>LEE, Gwo-Bin</creatorcontrib><title>Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification</title><title>Journal of microelectromechanical systems</title><addtitle>JMEMS</addtitle><description>This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator can also be used as microheating chambers to perform RT-PCR. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses or bacteria, the target virus and bacteria were recognized and further separated and purified from the biosamples by a magnetic field generated by the bioseparator. The target purified virus/bacteria was then lysed to release ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) for the subsequent RT-PCR processes. Experimental results showed that the target virus/bacteria was successfully separated and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA was automatically completed by utilizing the on-chip microheaters and the micro temperature sensor. The high mixing efficiency of the two-way s-shape pump and the rapid heating/cooling rate of the microheating chambers can significantly shorten the pretreatment and diagnosis processes. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast disease diagnosis.</description><subject>Applied fluid mechanics</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enrichment</subject><subject>Exact sciences and technology</subject><subject>Fluid dynamics</subject><subject>Fluidics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental areas of phenomenology (including applications)</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>In vitro gene amplification. Pcr technique</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>magnetic bead</subject><subject>Magnetic fields</subject><subject>Magnetic separation</subject><subject>Mechanical engineering. Machine design</subject><subject>Mechanical instruments, equipment and techniques</subject><subject>Methods. Procedures. Technologies</subject><subject>microelectromechanical systems (MEMS)</subject><subject>Microfluidics</subject><subject>Micromechanical devices and systems</subject><subject>Microorganisms</subject><subject>Micropumps</subject><subject>Physics</subject><subject>Polymers</subject><subject>Precision engineering, watch making</subject><subject>pretreatment</subject><subject>reverse-transcription polymerase chain reaction (RT-PCR)</subject><subject>RNA</subject><subject>Studies</subject><subject>Target recognition</subject><subject>Viruses</subject><subject>Viruses (medical)</subject><issn>1057-7157</issn><issn>1941-0158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNqFkk1v1DAQhiMEEqXwAxAXC4mPS5YZ24nt46q0UNQFpAVxjFxnDK6yyWI7SP33eEnVA4f2ZEt-5pHm9VtVzxFWiGDefdqcbrYrDqBXBltumgfVERqJNWCjH5Y7NKpW2KjH1ZOUrgBQSt0eVbQJLk5-mEMfHNtep0y7xM7HTD-jzdSzHyH_YpZt7W4_EPsaKUeyeUdjZu_pT3DE_BTZmU2ZfZ7dQMHVaxd6ti588MHZHKbxafXI2yHRs5vzuPp-dvrt5GN98eXD-cn6onZSY64dF0px7MFp1VvVenDecwQhLzVvWyUd-P7SmLa3xjdKoiHX8xZk4wT1GsRx9Wbx7uP0e6aUu11IjobBjjTNqTMgWg7S6HtJraGVCpQp5Os7SSGlbISCe0GOqMVifHsniMC5AcmbpqAv_0OvpjmOJcPOYElLlOULhAtUfjKlSL7bx7Cz8bqYukM7un_t6A7t6JZ2lJlXN2KbnB18tKML6XaQA1clz4P7xcIFIrp9LhsbBSj-ArqfwPQ</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>LIEN, Kang-Yi</creator><creator>LIN, Wang-Ying</creator><creator>LEE, Yu-Fang</creator><creator>WANG, Chih-Hao</creator><creator>LEI, Huan-Yao</creator><creator>LEE, Gwo-Bin</creator><general>IEEE</general><general>Institute of Electrical and Electronics Engineers</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7TM</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>F28</scope></search><sort><creationdate>20080401</creationdate><title>Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification</title><author>LIEN, Kang-Yi ; LIN, Wang-Ying ; LEE, Yu-Fang ; WANG, Chih-Hao ; LEI, Huan-Yao ; LEE, Gwo-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-c237721d0c87da76f0cff21034b826674c0fdb996da9f57419ecd26045c3ed803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Applied fluid mechanics</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Enrichment</topic><topic>Exact sciences and technology</topic><topic>Fluid dynamics</topic><topic>Fluidics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental areas of phenomenology (including applications)</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>In vitro gene amplification. Pcr technique</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>magnetic bead</topic><topic>Magnetic fields</topic><topic>Magnetic separation</topic><topic>Mechanical engineering. Machine design</topic><topic>Mechanical instruments, equipment and techniques</topic><topic>Methods. Procedures. Technologies</topic><topic>microelectromechanical systems (MEMS)</topic><topic>Microfluidics</topic><topic>Micromechanical devices and systems</topic><topic>Microorganisms</topic><topic>Micropumps</topic><topic>Physics</topic><topic>Polymers</topic><topic>Precision engineering, watch making</topic><topic>pretreatment</topic><topic>reverse-transcription polymerase chain reaction (RT-PCR)</topic><topic>RNA</topic><topic>Studies</topic><topic>Target recognition</topic><topic>Viruses</topic><topic>Viruses (medical)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIEN, Kang-Yi</creatorcontrib><creatorcontrib>LIN, Wang-Ying</creatorcontrib><creatorcontrib>LEE, Yu-Fang</creatorcontrib><creatorcontrib>WANG, Chih-Hao</creatorcontrib><creatorcontrib>LEI, Huan-Yao</creatorcontrib><creatorcontrib>LEE, Gwo-Bin</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Nucleic Acids Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><jtitle>Journal of microelectromechanical systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIEN, Kang-Yi</au><au>LIN, Wang-Ying</au><au>LEE, Yu-Fang</au><au>WANG, Chih-Hao</au><au>LEI, Huan-Yao</au><au>LEE, Gwo-Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification</atitle><jtitle>Journal of microelectromechanical systems</jtitle><stitle>JMEMS</stitle><date>2008-04-01</date><risdate>2008</risdate><volume>17</volume><issue>2</issue><spage>288</spage><epage>301</epage><pages>288-301</pages><issn>1057-7157</issn><eissn>1941-0158</eissn><coden>JMIYET</coden><abstract>This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator can also be used as microheating chambers to perform RT-PCR. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses or bacteria, the target virus and bacteria were recognized and further separated and purified from the biosamples by a magnetic field generated by the bioseparator. The target purified virus/bacteria was then lysed to release ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) for the subsequent RT-PCR processes. Experimental results showed that the target virus/bacteria was successfully separated and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA was automatically completed by utilizing the on-chip microheaters and the micro temperature sensor. The high mixing efficiency of the two-way s-shape pump and the rapid heating/cooling rate of the microheating chambers can significantly shorten the pretreatment and diagnosis processes. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast disease diagnosis.</abstract><cop>New York, NY</cop><pub>IEEE</pub><doi>10.1109/JMEMS.2008.916295</doi><tpages>14</tpages></addata></record> |
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| subjects | Applied fluid mechanics Applied sciences Biological and medical sciences Biotechnology Deoxyribonucleic acid DNA Enrichment Exact sciences and technology Fluid dynamics Fluidics Fundamental and applied biological sciences. Psychology Fundamental areas of phenomenology (including applications) Genetic engineering Genetic technics In vitro gene amplification. Pcr technique Instruments, apparatus, components and techniques common to several branches of physics and astronomy magnetic bead Magnetic fields Magnetic separation Mechanical engineering. Machine design Mechanical instruments, equipment and techniques Methods. Procedures. Technologies microelectromechanical systems (MEMS) Microfluidics Micromechanical devices and systems Microorganisms Micropumps Physics Polymers Precision engineering, watch making pretreatment reverse-transcription polymerase chain reaction (RT-PCR) RNA Studies Target recognition Viruses Viruses (medical) |
| title | Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification |
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