Transient expression of the uidA gene in Pinus pinea cotyledons: a study of heterologous promoter sequences

Transfer and expression of the beta-glucuronidase gene (uidA) in cultured cotyledons of stone pine (Pinus pinea L.) was obtained by microprojectile bombardment. Conditions for optimum transient expression were established by using plasmid pBI121 delivered by 1.0 micrometer-diameter gold particles, i...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant cell, tissue and organ culture tissue and organ culture, 1999, Vol.56 (2), p.69-78
Hauptverfasser:	Humara, J.M, Lopez, M, Ordas, R.J
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-glucuronidase Biological and medical sciences Biotechnology cotyledons developmental stages enzyme activity Fundamental and applied biological sciences. Psychology gene expression Gene transfer genes Genetic engineering Genetic technics genetic transformation histochemistry in vitro culture localization Methods. Procedures. Technologies Pinus pinea promoter regions regenerative ability Synthetic digonucleotides and genes. Sequencing transgenic plants
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!