Green-Tissue-Specific Expression of a Reconstructed cry1C Gene Encoding the Active Fragment of Bacillus thuringiensis δ-Endotoxin in Haploid Tobacco Plants Conferring Resistance to Spodoptera litura
The DNA sequence of a truncated cry1C gene encoding the active fragment of Bacillus thuringiensis (Bt) δ-endotoxin was fully reconstructed by introduction of silent mutations. Each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into hap...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-08, Vol.63 (8), p.1433-1444 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | CHRISTOV, Nikolai Kirilov IMAISHI, Hiromasa OHKAWA, Hideo |
| description | The DNA sequence of a truncated cry1C gene encoding the active fragment of Bacillus thuringiensis (Bt) δ-endotoxin was fully reconstructed by introduction of silent mutations. Each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into haploid tobacco plants under the control of the rbcS promoter. To facilitate selection of transgenic tobacco plants with high insecticidal activity, a fusion gene encoding both rat CYP1A1 cytochrome P450 and yeast NADPH-P450 oxidoreductase was cotransformed with the wild type cry1C gene. The synthetic gene elevated the levels of Cry1C protein and the mRNA in transgenic tobacco plants as well as mortality in Spodoptera litura larvae. The Cry1C protein was accumulated mainly in the leaf tissues of the transgenic tobacco plants. The results reported here imply that the green-tissue-specific expression of the synthetic cry1C gene is useful for the control of S. litura which was rather resistant to the other types of Bt toxins. |
| doi_str_mv | 10.1271/bbb.63.1433 |
| format | Article |
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Each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into haploid tobacco plants under the control of the rbcS promoter. To facilitate selection of transgenic tobacco plants with high insecticidal activity, a fusion gene encoding both rat CYP1A1 cytochrome P450 and yeast NADPH-P450 oxidoreductase was cotransformed with the wild type cry1C gene. The synthetic gene elevated the levels of Cry1C protein and the mRNA in transgenic tobacco plants as well as mortality in Spodoptera litura larvae. The Cry1C protein was accumulated mainly in the leaf tissues of the transgenic tobacco plants. The results reported here imply that the green-tissue-specific expression of the synthetic cry1C gene is useful for the control of S. litura which was rather resistant to the other types of Bt toxins.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.63.1433</identifier><identifier>PMID: 10501003</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; Animals ; Bacillus thuringiensis ; Bacillus thuringiensis Toxins ; Bacterial Proteins - chemistry ; Bacterial Toxins ; Base Sequence ; Biological and medical sciences ; Biotechnology ; cry1C ; cry1C gene ; Cry1C protein ; Cry1C toxin ; Cryptochromes ; Cytochrome P-450 CYP1A1 - genetics ; d-endotoxin ; Drosophila Proteins ; Drug Resistance ; Endotoxins - chemistry ; Eye Proteins ; Flavoproteins - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic Code ; Genetic engineering ; Genetic technics ; Haploidy ; Hemolysin Proteins ; insect resistance ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana tabacum ; Pest Control, Biological ; Photoreceptor Cells, Invertebrate ; Plants, Genetically Modified ; Plants, Toxic ; Protein Isoforms - genetics ; Rats ; Receptors, G-Protein-Coupled ; Spodoptera ; Spodoptera - pathogenicity ; Spodoptera litura ; Transformation, Genetic ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1999-08, Vol.63 (8), p.1433-1444</ispartof><rights>1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-85642890c3818f72c9e07293e1dcd849940ae66877a4bea28be923a469c647773</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1987272$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10501003$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHRISTOV, Nikolai Kirilov</creatorcontrib><creatorcontrib>IMAISHI, Hiromasa</creatorcontrib><creatorcontrib>OHKAWA, Hideo</creatorcontrib><title>Green-Tissue-Specific Expression of a Reconstructed cry1C Gene Encoding the Active Fragment of Bacillus thuringiensis δ-Endotoxin in Haploid Tobacco Plants Conferring Resistance to Spodoptera litura</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The DNA sequence of a truncated cry1C gene encoding the active fragment of Bacillus thuringiensis (Bt) δ-endotoxin was fully reconstructed by introduction of silent mutations. Each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into haploid tobacco plants under the control of the rbcS promoter. To facilitate selection of transgenic tobacco plants with high insecticidal activity, a fusion gene encoding both rat CYP1A1 cytochrome P450 and yeast NADPH-P450 oxidoreductase was cotransformed with the wild type cry1C gene. The synthetic gene elevated the levels of Cry1C protein and the mRNA in transgenic tobacco plants as well as mortality in Spodoptera litura larvae. The Cry1C protein was accumulated mainly in the leaf tissues of the transgenic tobacco plants. The results reported here imply that the green-tissue-specific expression of the synthetic cry1C gene is useful for the control of S. litura which was rather resistant to the other types of Bt toxins.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis Toxins</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Toxins</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cry1C</subject><subject>cry1C gene</subject><subject>Cry1C protein</subject><subject>Cry1C toxin</subject><subject>Cryptochromes</subject><subject>Cytochrome P-450 CYP1A1 - genetics</subject><subject>d-endotoxin</subject><subject>Drosophila Proteins</subject><subject>Drug Resistance</subject><subject>Endotoxins - chemistry</subject><subject>Eye Proteins</subject><subject>Flavoproteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Code</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Haploidy</subject><subject>Hemolysin Proteins</subject><subject>insect resistance</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Pest Control, Biological</subject><subject>Photoreceptor Cells, Invertebrate</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>Protein Isoforms - genetics</subject><subject>Rats</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Spodoptera</subject><subject>Spodoptera - pathogenicity</subject><subject>Spodoptera litura</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkcGKFDEQhoMo7rh68i45iBfpMemkk85xHWZnhQXFHc9NOl29RjJJm6R15718B2_7TJthRvQgFBRUfX_xUz9CLylZ0lrSd33fLwVbUs7YI7SgjMtKKC4fowVRVFQtb-gZepbSN0LKoKFP0RklDaGEsAX6vYkAvtralGaobiYwdrQGr--mCCnZ4HEYscafwQSfcpxNhgGbuKcrvAEPeO1NGKy_xfkr4AuT7Q_Al1Hf7sDng_S9Nta5OZX9HAtnwSeb8P2vau2HkMOd9bjUlZ5csAPehl4bE_Anp31OeBX8CPGgKw6KLmtvAOeAb6YwhClD1NjZPEf9HD0ZtUvw4tTP0ZfL9XZ1VV1_3HxYXVxXhtdNrtpG8LpVxLCWtqOsjQIia8WADmZouVKcaBCilVLzHnTd9qBqprlQRnApJTtHb453pxi-z5Byt7PJgCt-Icypo5LLRhFWwLdH0MSQUoSxm6Ld6bjvKOkOuXUlt06w7pBboV-dzs79DoZ_2GNQBXh9AnQy2o2xfMKmv5xqZS3rgokjZv0Y4k7_DNENXdZ7F-IfDfufgQcWobaQ</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>CHRISTOV, Nikolai Kirilov</creator><creator>IMAISHI, Hiromasa</creator><creator>OHKAWA, Hideo</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19990801</creationdate><title>Green-Tissue-Specific Expression of a Reconstructed cry1C Gene Encoding the Active Fragment of Bacillus thuringiensis δ-Endotoxin in Haploid Tobacco Plants Conferring Resistance to Spodoptera litura</title><author>CHRISTOV, Nikolai Kirilov ; IMAISHI, Hiromasa ; OHKAWA, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-85642890c3818f72c9e07293e1dcd849940ae66877a4bea28be923a469c647773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis Toxins</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Toxins</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cry1C</topic><topic>cry1C gene</topic><topic>Cry1C protein</topic><topic>Cry1C toxin</topic><topic>Cryptochromes</topic><topic>Cytochrome P-450 CYP1A1 - genetics</topic><topic>d-endotoxin</topic><topic>Drosophila Proteins</topic><topic>Drug Resistance</topic><topic>Endotoxins - chemistry</topic><topic>Eye Proteins</topic><topic>Flavoproteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Code</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Haploidy</topic><topic>Hemolysin Proteins</topic><topic>insect resistance</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana tabacum</topic><topic>Pest Control, Biological</topic><topic>Photoreceptor Cells, Invertebrate</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>Protein Isoforms - genetics</topic><topic>Rats</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Spodoptera</topic><topic>Spodoptera - pathogenicity</topic><topic>Spodoptera litura</topic><topic>Transformation, Genetic</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHRISTOV, Nikolai Kirilov</creatorcontrib><creatorcontrib>IMAISHI, Hiromasa</creatorcontrib><creatorcontrib>OHKAWA, Hideo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHRISTOV, Nikolai Kirilov</au><au>IMAISHI, Hiromasa</au><au>OHKAWA, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green-Tissue-Specific Expression of a Reconstructed cry1C Gene Encoding the Active Fragment of Bacillus thuringiensis δ-Endotoxin in Haploid Tobacco Plants Conferring Resistance to Spodoptera litura</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>63</volume><issue>8</issue><spage>1433</spage><epage>1444</epage><pages>1433-1444</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The DNA sequence of a truncated cry1C gene encoding the active fragment of Bacillus thuringiensis (Bt) δ-endotoxin was fully reconstructed by introduction of silent mutations. Each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into haploid tobacco plants under the control of the rbcS promoter. To facilitate selection of transgenic tobacco plants with high insecticidal activity, a fusion gene encoding both rat CYP1A1 cytochrome P450 and yeast NADPH-P450 oxidoreductase was cotransformed with the wild type cry1C gene. The synthetic gene elevated the levels of Cry1C protein and the mRNA in transgenic tobacco plants as well as mortality in Spodoptera litura larvae. The Cry1C protein was accumulated mainly in the leaf tissues of the transgenic tobacco plants. The results reported here imply that the green-tissue-specific expression of the synthetic cry1C gene is useful for the control of S. litura which was rather resistant to the other types of Bt toxins.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>10501003</pmid><doi>10.1271/bbb.63.1433</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1999-08, Vol.63 (8), p.1433-1444 |
| issn | 0916-8451 1347-6947 |
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| recordid | cdi_pascalfrancis_primary_1987272 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Animals Bacillus thuringiensis Bacillus thuringiensis Toxins Bacterial Proteins - chemistry Bacterial Toxins Base Sequence Biological and medical sciences Biotechnology cry1C cry1C gene Cry1C protein Cry1C toxin Cryptochromes Cytochrome P-450 CYP1A1 - genetics d-endotoxin Drosophila Proteins Drug Resistance Endotoxins - chemistry Eye Proteins Flavoproteins - genetics Fundamental and applied biological sciences. Psychology Genetic Code Genetic engineering Genetic technics Haploidy Hemolysin Proteins insect resistance Methods. Procedures. Technologies Molecular Sequence Data Nicotiana - genetics Nicotiana tabacum Pest Control, Biological Photoreceptor Cells, Invertebrate Plants, Genetically Modified Plants, Toxic Protein Isoforms - genetics Rats Receptors, G-Protein-Coupled Spodoptera Spodoptera - pathogenicity Spodoptera litura Transformation, Genetic Transgenic animals and transgenic plants Transgenic plants |
| title | Green-Tissue-Specific Expression of a Reconstructed cry1C Gene Encoding the Active Fragment of Bacillus thuringiensis δ-Endotoxin in Haploid Tobacco Plants Conferring Resistance to Spodoptera litura |
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