Alu-Primed Polymerase Chain Reaction for Regional Assignment of 110 Yeast Artificial Chromosome Clones from the Human X Chromosome: Identification of Clones Associated with a Disease Locus

Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints. Polymerase chain reaction using Alu primers was applied to YAC clones in order to generate probes, to i...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (14), p.6157-6161
Hauptverfasser:	Nelson, David L., Ballabio, Andrea, Victoria, Maureen F., Pieretti, Maura, Bies, Roger D., Gibbs, Richard A., Maley, Julie A., Chinault, A. Craig, Webster, Thomas D., Caskey, C. Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	550400 - Genetics Base Sequence BASIC BIOLOGICAL SCIENCES Biological and medical sciences Blotting, Southern Cell lines CHROMOSOMAL ABERRATIONS Chromosome Mapping CHROMOSOMES Chromosomes, Fungal Classical genetics, quantitative genetics, hybrids CLONING Cloning, Molecular Deoxyribonucleases, Type II Site-Specific DISEASES DNA DNA HYBRIDIZATION DNA POLYMERASES DNA probes DNA-CLONING ENZYMES EUMYCOTA Fundamental and applied biological sciences. Psychology FUNGI Gene Library Genetic Diseases, Inborn - genetics GENETIC MAPPING Genetic vectors Genetics of eukaryotes. Biological and molecular evolution HEREDITARY DISEASES HETEROCHROMOSOMES Human HUMAN X CHROMOSOME Humans Hybrid cells HYBRIDIZATION Libraries Lowe syndrome MAPPING MICROORGANISMS Molecular Sequence Data MUTATIONS Nucleotide Mapping NUCLEOTIDYLTRANSFERASES Oculocerebrorenal syndrome Oligonucleotide Probes ORGANIC COMPOUNDS PHOSPHORUS-GROUP TRANSFERASES PLANTS Polymerase chain reaction Polymerase Chain Reaction - methods POLYMERASES PROTEINS Restriction Mapping Saccharomyces cerevisiae - genetics TRANSFERASES TRANSLOCATION X CHROMOSOME YEASTS
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