Affinity partitioning of restriction endonucleases : Application to the purification of EcoR I and EcoR V

Partitioning of restriction endonucleases between two liquid aqueous phases can be strongly influenced by group-specific ligands included in the two-phase system. Three restriction endonucleases, namely EcoR I, EcoR V and BamH I, were partitioned within an aqueous dextran—polyethylene glycol (PEG) s...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of Chromatography A 1991-02, Vol.538 (2), p.311-321
Hauptverfasser:	Vlatakis, G., Bouriotis, V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Affinity Deoxyribonuclease BamHI - isolation & purification Deoxyribonuclease EcoRI - isolation & purification Deoxyribonuclease EcoRI - metabolism Deoxyribonucleases, Type II Site-Specific - isolation & purification Deoxyribonucleases, Type II Site-Specific - metabolism DNA - metabolism Enzymes and enzyme inhibitors Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Sodium Chloride Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!