Monoclonal Antibodies to the Apical Chloride Channel in Necturus Gallbladder Inhibit the Chloride Conductance
Monoclonal antibodies raised by injecting Necturus gallbladder cells into mice were tested for their ability to inhibit the apical chloride conductance induced by elevation of cellular cAMP. Five of these monoclonal antibodies bound to the apical cells, as shown by indirect immunofluorescence micros...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (19), p.7649-7652 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Finn, Arthur L. Tsai, Lih-Min Falk, Ronald J. |
| description | Monoclonal antibodies raised by injecting Necturus gallbladder cells into mice were tested for their ability to inhibit the apical chloride conductance induced by elevation of cellular cAMP. Five of these monoclonal antibodies bound to the apical cells, as shown by indirect immunofluorescence microscopy, and inhibited the chloride conductance; one antibody that bound only to subepithelial smooth muscle, by indirect immunofluorescence microscopy, showed no inhibition of chloride transport. The channel or a closely related molecule is present in the membrane whether or not the pathway is open, since, in addition to inhibiting the conductance of the open channel, the antibody also bound to the membrane in the resting state and prevented subsequent opening of the channel. The antibody was shown to recognize, by ELISA, epitopes from the Necturus gallbladder and small intestine. Finally, by Western blot analysis of Necturus gallbladder homogenates, the antibody was shown to recognize two protein bands of Mr 219,000 and Mr 69,000. This antibody should permit isolation and characterization of this important ion channel. |
| doi_str_mv | 10.1073/pnas.86.19.7649 |
| format | Article |
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Five of these monoclonal antibodies bound to the apical cells, as shown by indirect immunofluorescence microscopy, and inhibited the chloride conductance; one antibody that bound only to subepithelial smooth muscle, by indirect immunofluorescence microscopy, showed no inhibition of chloride transport. The channel or a closely related molecule is present in the membrane whether or not the pathway is open, since, in addition to inhibiting the conductance of the open channel, the antibody also bound to the membrane in the resting state and prevented subsequent opening of the channel. The antibody was shown to recognize, by ELISA, epitopes from the Necturus gallbladder and small intestine. Finally, by Western blot analysis of Necturus gallbladder homogenates, the antibody was shown to recognize two protein bands of Mr 219,000 and Mr 69,000. This antibody should permit isolation and characterization of this important ion channel.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.19.7649</identifier><identifier>PMID: 2477847</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antibodies, Monoclonal ; Antigens ; Antigens, Surface - analysis ; Biological and medical sciences ; Cell physiology ; chloride ; Chloride Channels ; Chlorides ; Chlorides - immunology ; Electric Conductivity ; Enzyme-Linked Immunosorbent Assay ; Epithelial cells ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gallbladder ; Gallbladder - physiology ; Ion Channels - physiology ; Membrane and intracellular transports ; Membrane Proteins - immunology ; Membrane Proteins - physiology ; Microscopy ; Molecular and cellular biology ; Monoclonal antibodies ; Necturus ; Room temperature ; Smooth muscle</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-10, Vol.86 (19), p.7649-7652</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-42e31bdf9b39d28528b498a6057f3808dbefd76a6d11b5eca86a265bf6f1cb973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/34706$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/34706$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19351496$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2477847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Finn, Arthur L.</creatorcontrib><creatorcontrib>Tsai, Lih-Min</creatorcontrib><creatorcontrib>Falk, Ronald J.</creatorcontrib><title>Monoclonal Antibodies to the Apical Chloride Channel in Necturus Gallbladder Inhibit the Chloride Conductance</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Monoclonal antibodies raised by injecting Necturus gallbladder cells into mice were tested for their ability to inhibit the apical chloride conductance induced by elevation of cellular cAMP. Five of these monoclonal antibodies bound to the apical cells, as shown by indirect immunofluorescence microscopy, and inhibited the chloride conductance; one antibody that bound only to subepithelial smooth muscle, by indirect immunofluorescence microscopy, showed no inhibition of chloride transport. The channel or a closely related molecule is present in the membrane whether or not the pathway is open, since, in addition to inhibiting the conductance of the open channel, the antibody also bound to the membrane in the resting state and prevented subsequent opening of the channel. The antibody was shown to recognize, by ELISA, epitopes from the Necturus gallbladder and small intestine. Finally, by Western blot analysis of Necturus gallbladder homogenates, the antibody was shown to recognize two protein bands of Mr 219,000 and Mr 69,000. This antibody should permit isolation and characterization of this important ion channel.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>chloride</subject><subject>Chloride Channels</subject><subject>Chlorides</subject><subject>Chlorides - immunology</subject><subject>Electric Conductivity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epithelial cells</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gallbladder</subject><subject>Gallbladder - physiology</subject><subject>Ion Channels - physiology</subject><subject>Membrane and intracellular transports</subject><subject>Membrane Proteins - immunology</subject><subject>Membrane Proteins - physiology</subject><subject>Microscopy</subject><subject>Molecular and cellular biology</subject><subject>Monoclonal antibodies</subject><subject>Necturus</subject><subject>Room temperature</subject><subject>Smooth muscle</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP0zAUhSMEGsrAGgkJlA2wSsd2HD8WLKoKhpEG2MDa8ivUI9cutoPg3-PQ0GE2sPKVznfuw6dpnkKwhoD2F4cg85qRNeRrSjC_16wg4LCrJbjfrABAtGMY4YfNo5xvAAB8YOCsOUOYUobpqtl_iCFqH4P07SYUp6JxNrcltmVn283B6Spsdz4mZ2wtZAjWty60H60uU5pyeym9V14aY1N7FXZOufLbe2uKwUy6yKDt4-bBKH22T5b3vPny7u3n7fvu-tPl1XZz3ekBodJhZHuozMhVzw1iA2IKcyYJGOjYM8CMsqOhRBIDoRqsloxIRAY1khFqxWl_3rw59j1Mam-NtqEk6cUhub1MP0WUTtxVgtuJr_G7QJxBhKv_1eJP8dtkcxF7l7X1XgYbpywoRwPgjP0XhANmFNB5o4sjqFPMOdnxtAwEYk5SzEkKRgTkYk6yOp7_fcOJX6Kr-stFl7mGNKb6wS7ftuX9ADEnlXuxcPOAP_KdQa__CYhx8r7YH6WSz47kTS4xndAeU0D6X1_qyno</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Finn, Arthur L.</creator><creator>Tsai, Lih-Min</creator><creator>Falk, Ronald J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891001</creationdate><title>Monoclonal Antibodies to the Apical Chloride Channel in Necturus Gallbladder Inhibit the Chloride Conductance</title><author>Finn, Arthur L. ; Tsai, Lih-Min ; Falk, Ronald J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-42e31bdf9b39d28528b498a6057f3808dbefd76a6d11b5eca86a265bf6f1cb973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>chloride</topic><topic>Chloride Channels</topic><topic>Chlorides</topic><topic>Chlorides - immunology</topic><topic>Electric Conductivity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelial cells</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gallbladder</topic><topic>Gallbladder - physiology</topic><topic>Ion Channels - physiology</topic><topic>Membrane and intracellular transports</topic><topic>Membrane Proteins - immunology</topic><topic>Membrane Proteins - physiology</topic><topic>Microscopy</topic><topic>Molecular and cellular biology</topic><topic>Monoclonal antibodies</topic><topic>Necturus</topic><topic>Room temperature</topic><topic>Smooth muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finn, Arthur L.</creatorcontrib><creatorcontrib>Tsai, Lih-Min</creatorcontrib><creatorcontrib>Falk, Ronald J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finn, Arthur L.</au><au>Tsai, Lih-Min</au><au>Falk, Ronald J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Antibodies to the Apical Chloride Channel in Necturus Gallbladder Inhibit the Chloride Conductance</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>86</volume><issue>19</issue><spage>7649</spage><epage>7652</epage><pages>7649-7652</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Monoclonal antibodies raised by injecting Necturus gallbladder cells into mice were tested for their ability to inhibit the apical chloride conductance induced by elevation of cellular cAMP. Five of these monoclonal antibodies bound to the apical cells, as shown by indirect immunofluorescence microscopy, and inhibited the chloride conductance; one antibody that bound only to subepithelial smooth muscle, by indirect immunofluorescence microscopy, showed no inhibition of chloride transport. The channel or a closely related molecule is present in the membrane whether or not the pathway is open, since, in addition to inhibiting the conductance of the open channel, the antibody also bound to the membrane in the resting state and prevented subsequent opening of the channel. The antibody was shown to recognize, by ELISA, epitopes from the Necturus gallbladder and small intestine. Finally, by Western blot analysis of Necturus gallbladder homogenates, the antibody was shown to recognize two protein bands of Mr 219,000 and Mr 69,000. This antibody should permit isolation and characterization of this important ion channel.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2477847</pmid><doi>10.1073/pnas.86.19.7649</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Antibodies, Monoclonal Antigens Antigens, Surface - analysis Biological and medical sciences Cell physiology chloride Chloride Channels Chlorides Chlorides - immunology Electric Conductivity Enzyme-Linked Immunosorbent Assay Epithelial cells Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gallbladder Gallbladder - physiology Ion Channels - physiology Membrane and intracellular transports Membrane Proteins - immunology Membrane Proteins - physiology Microscopy Molecular and cellular biology Monoclonal antibodies Necturus Room temperature Smooth muscle |
| title | Monoclonal Antibodies to the Apical Chloride Channel in Necturus Gallbladder Inhibit the Chloride Conductance |
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