Outcome of Colonization of Apis mellifera by Nosema ceranae

A multiplex PCR-based method, in which two small-subunit rRNA regions are simultaneously amplified in a single reaction, was designed for parallel detection of honeybee microsporidians (Nosema apis and Nosema ceranae). Each of two pairs of primers exclusively amplified the 16S rRNA targeted gene of...

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Veröffentlicht in:	Applied and Environmental Microbiology 2007-10, Vol.73 (20), p.6331-6338
Hauptverfasser:	Martín-Hernández, Raquel, Meana, Aránzazu, Prieto, Lourdes, Salvador, Amparo Martínez, Garrido-Bailón, Encarna, Higes, Mariano
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apis Apis mellifera Bees Bees - growth & development Bees - microbiology Biological and medical sciences Diagnostic tests DNA, Fungal - analysis DNA, Fungal - isolation & purification Fundamental and applied biological sciences. Psychology Genes Invertebrate Microbiology Microbiology Molecular Sequence Data Nosema Nosema - classification Nosema - genetics Nosema - isolation & purification Nosema - physiology Nosema apis Parasites Polymerase Chain Reaction Reproducibility of Results Ribonucleic acid RNA Sensitivity and Specificity Sequence Analysis, DNA Species Specificity Spores, Fungal - classification Spores, Fungal - genetics Spores, Fungal - isolation & purification Spores, Fungal - physiology
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