Purification and analysis of a 5 kDa component of enamel matrix derivative
High performance liquid chromatography (HPLC) methods were used to analyse a 5 kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain ®, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC)...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-10, Vol.857 (2), p.210-218 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Mumulidu, Alexandra Hildebrand, Bergisa Fabi, Beata Hammarström, Lars Cochran, David L. Dard, Michel Lemoult, Stephanie |
| description | High performance liquid chromatography (HPLC) methods were used to analyse a 5
kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain
®, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100
cm
×
5
cm column (Bio-Gel P-30 Fine, 280
nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220
nm). The fractions containing only the 5
kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200
nm), revealing four peaks of the 5
kDa component. From 1200
mg of EMD (of which 9% is the 5
kDa component), approximately 65
mg of lyophilised 5
kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis. |
| doi_str_mv | 10.1016/j.jchromb.2007.07.017 |
| format | Article |
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kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain
®, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100
cm
×
5
cm column (Bio-Gel P-30 Fine, 280
nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220
nm). The fractions containing only the 5
kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200
nm), revealing four peaks of the 5
kDa component. From 1200
mg of EMD (of which 9% is the 5
kDa component), approximately 65
mg of lyophilised 5
kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2007.07.017</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Emdogain ; Enamel matrix derivative ; Fractionation ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Medical sciences ; Method validation ; Pharmacology. Drug treatments ; Protein purification ; Qualitative analysis ; Quantitative analysis ; Reversed-phase HPLC ; Size-exclusion HPLC</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-10, Vol.857 (2), p.210-218</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2007.07.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19100958$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Mumulidu, Alexandra</creatorcontrib><creatorcontrib>Hildebrand, Bergisa</creatorcontrib><creatorcontrib>Fabi, Beata</creatorcontrib><creatorcontrib>Hammarström, Lars</creatorcontrib><creatorcontrib>Cochran, David L.</creatorcontrib><creatorcontrib>Dard, Michel</creatorcontrib><creatorcontrib>Lemoult, Stephanie</creatorcontrib><title>Purification and analysis of a 5 kDa component of enamel matrix derivative</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><description>High performance liquid chromatography (HPLC) methods were used to analyse a 5
kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain
®, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100
cm
×
5
cm column (Bio-Gel P-30 Fine, 280
nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220
nm). The fractions containing only the 5
kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200
nm), revealing four peaks of the 5
kDa component. From 1200
mg of EMD (of which 9% is the 5
kDa component), approximately 65
mg of lyophilised 5
kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Emdogain</subject><subject>Enamel matrix derivative</subject><subject>Fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Method validation</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein purification</subject><subject>Qualitative analysis</subject><subject>Quantitative analysis</subject><subject>Reversed-phase HPLC</subject><subject>Size-exclusion HPLC</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNotkE1LxEAMhgdRcF39CcJcPLYmnbbTnkRW1w8W9KDgbUinU5zaj2WmLu6_d8ouJCQkT0LyMnaNECNgftvGrf52Y1_FCYCMZ0N5whZYSBEJmX-dhjyTEEEiknN24X0LgQApFuz1_dfZxmqa7DhwGurg1O299XxsOPGM_zwQ12O_HQczTHPRDNSbjvc0OfvHa-PsLkzvzCU7a6jz5uoYl-xz_fixeo42b08vq_tNZJIUp6gsJOZYplUOWVNTjVWVikpAoXVhJAhIM0QhZV5msjK5DE0JZSKK8FyZCRJLdnPYuyWvqWscDdp6tXW2J7dXWOIMFoG7O3AmHLOzximvrRm0qa0zelL1aBWCmiVUrTpKqGYJ1WwoxT-4ymXq</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Mumulidu, Alexandra</creator><creator>Hildebrand, Bergisa</creator><creator>Fabi, Beata</creator><creator>Hammarström, Lars</creator><creator>Cochran, David L.</creator><creator>Dard, Michel</creator><creator>Lemoult, Stephanie</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope></search><sort><creationdate>20071001</creationdate><title>Purification and analysis of a 5 kDa component of enamel matrix derivative</title><author>Mumulidu, Alexandra ; Hildebrand, Bergisa ; Fabi, Beata ; Hammarström, Lars ; Cochran, David L. ; Dard, Michel ; Lemoult, Stephanie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e241t-98716194b605fdad1bb43b308cc8e703045113776957be6743b709238200953a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Emdogain</topic><topic>Enamel matrix derivative</topic><topic>Fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Method validation</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein purification</topic><topic>Qualitative analysis</topic><topic>Quantitative analysis</topic><topic>Reversed-phase HPLC</topic><topic>Size-exclusion HPLC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mumulidu, Alexandra</creatorcontrib><creatorcontrib>Hildebrand, Bergisa</creatorcontrib><creatorcontrib>Fabi, Beata</creatorcontrib><creatorcontrib>Hammarström, Lars</creatorcontrib><creatorcontrib>Cochran, David L.</creatorcontrib><creatorcontrib>Dard, Michel</creatorcontrib><creatorcontrib>Lemoult, Stephanie</creatorcontrib><collection>Pascal-Francis</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mumulidu, Alexandra</au><au>Hildebrand, Bergisa</au><au>Fabi, Beata</au><au>Hammarström, Lars</au><au>Cochran, David L.</au><au>Dard, Michel</au><au>Lemoult, Stephanie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and analysis of a 5 kDa component of enamel matrix derivative</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><date>2007-10-01</date><risdate>2007</risdate><volume>857</volume><issue>2</issue><spage>210</spage><epage>218</epage><pages>210-218</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>High performance liquid chromatography (HPLC) methods were used to analyse a 5
kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain
®, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100
cm
×
5
cm column (Bio-Gel P-30 Fine, 280
nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220
nm). The fractions containing only the 5
kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200
nm), revealing four peaks of the 5
kDa component. From 1200
mg of EMD (of which 9% is the 5
kDa component), approximately 65
mg of lyophilised 5
kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.jchromb.2007.07.017</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-10, Vol.857 (2), p.210-218 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_pascalfrancis_primary_19100958 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Emdogain Enamel matrix derivative Fractionation Fundamental and applied biological sciences. Psychology General pharmacology Medical sciences Method validation Pharmacology. Drug treatments Protein purification Qualitative analysis Quantitative analysis Reversed-phase HPLC Size-exclusion HPLC |
| title | Purification and analysis of a 5 kDa component of enamel matrix derivative |
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