ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1)
The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambdagt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female...
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| Veröffentlicht in: | Archives of andrology 1999, Vol.42 (3), p.127-136 |
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| creator | WU, Y.W. CHEN, D.H. MIAO, S. Y. WANG, L.F. ZONG, S. D. KOIDE, S.S. |
| description | The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambdagt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female mice were inoculated intramuscularly with the plasmid DNA and the expression of HSD-1 was determined. HSD-1 mRNAs were detected in myocytes and endomysial connective tissue cells of the quadriceps muscle by in situ hybridization. Spleen of inoculated animals contained an increased number of cytotoxic T lymphocytes, phagocytes, and plasma cells. Fertility of the treated animals was not affected. Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response. |
| doi_str_mv | 10.1080/014850199262788 |
| format | Article |
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Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0148-5016</identifier><identifier>EISSN: 1362-3095</identifier><identifier>EISSN: 1521-0375</identifier><identifier>DOI: 10.1080/014850199262788</identifier><identifier>PMID: 10407643</identifier><identifier>CODEN: ARANDR</identifier><language>eng</language><publisher>Philadelphia, PA: Informa UK Ltd</publisher><subject>Animals ; Antigens, Surface ; Biological and medical sciences ; Connective Tissue - metabolism ; Cytotoxic t Lymphocytes;Dna Vaccine;Fertility;Sperm Component ; Female ; Fertility ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Transfer Techniques ; Genetics of the immune response ; Humans ; Immunobiology ; In Situ Hybridization ; Male ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Mice ; Molecular Sequence Data ; Muscle, Skeletal - metabolism ; Phagocytes - cytology ; Plasmids - immunology ; Spermatozoa - metabolism ; Spleen - immunology ; T-Lymphocytes, Cytotoxic - cytology ; T-Lymphocytes, Cytotoxic - immunology ; Transcription, Genetic</subject><ispartof>Archives of andrology, 1999, Vol.42 (3), p.127-136</ispartof><rights>1999 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-2204b88eff50a9a1d8fb290c625192e6ce0ecb9fafc7782c1e81a8007a61cd7a3</citedby><cites>FETCH-LOGICAL-c493t-2204b88eff50a9a1d8fb290c625192e6ce0ecb9fafc7782c1e81a8007a61cd7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/014850199262788$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/014850199262788$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1883955$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10407643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WU, Y.W.</creatorcontrib><creatorcontrib>CHEN, D.H.</creatorcontrib><creatorcontrib>MIAO, S. Y.</creatorcontrib><creatorcontrib>WANG, L.F.</creatorcontrib><creatorcontrib>ZONG, S. D.</creatorcontrib><creatorcontrib>KOIDE, S.S.</creatorcontrib><title>ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1)</title><title>Archives of andrology</title><addtitle>Arch Androl</addtitle><description>The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambdagt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female mice were inoculated intramuscularly with the plasmid DNA and the expression of HSD-1 was determined. HSD-1 mRNAs were detected in myocytes and endomysial connective tissue cells of the quadriceps muscle by in situ hybridization. Spleen of inoculated animals contained an increased number of cytotoxic T lymphocytes, phagocytes, and plasma cells. Fertility of the treated animals was not affected. Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response.</description><subject>Animals</subject><subject>Antigens, Surface</subject><subject>Biological and medical sciences</subject><subject>Connective Tissue - metabolism</subject><subject>Cytotoxic t Lymphocytes;Dna Vaccine;Fertility;Sperm Component</subject><subject>Female</subject><subject>Fertility</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Transfer Techniques</subject><subject>Genetics of the immune response</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Phagocytes - cytology</subject><subject>Plasmids - immunology</subject><subject>Spermatozoa - metabolism</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes, Cytotoxic - cytology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Transcription, Genetic</subject><issn>0955-3002</issn><issn>0148-5016</issn><issn>1362-3095</issn><issn>1521-0375</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPAjEURhujUXys3ZkujNHFyG3n1bpDGGESZiAMLFxNSmkjZmCwhRj_vVUwPhI3t3dxvi-3B6FzArcEGDSBBCwEwjmNaMzYHmoQP6KeDzzcRw03Q7cDPULH1j6D28Bnh-iIQABxFPgNNEr6aTsdp3kXt3KcZtkkT_AoKYaDvEjw_SMe9ltFlnZwJ2_hJG8POp8o7k0yxxfDZJTh4WgwTtIcX_eKjkduTtGBFpVVZ7v3BE0eknG75_UH3bTd6nsy4P7aoxSCKWNK6xAEF2TG9JRykBENCacqkgqUnHIttIxjRiVRjAgGEIuIyFks_BN0te1dmfplo-y6XMytVFUllqre2DLijFPgkQObW1Ca2lqjdLky84UwbyWB8kNj-UejS1zsqjfThZr94LfeHHC5A4SVotJGLOXcfnOuw7l32N0Wmy91bRbitTbVrFyLt6o2Xxn__yP4r_CTEtX6SQqjyud6Y5bO7b8feAf9S5WW</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>WU, Y.W.</creator><creator>CHEN, D.H.</creator><creator>MIAO, S. Y.</creator><creator>WANG, L.F.</creator><creator>ZONG, S. D.</creator><creator>KOIDE, S.S.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1)</title><author>WU, Y.W. ; CHEN, D.H. ; MIAO, S. Y. ; WANG, L.F. ; ZONG, S. D. ; KOIDE, S.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-2204b88eff50a9a1d8fb290c625192e6ce0ecb9fafc7782c1e81a8007a61cd7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antigens, Surface</topic><topic>Biological and medical sciences</topic><topic>Connective Tissue - metabolism</topic><topic>Cytotoxic t Lymphocytes;Dna Vaccine;Fertility;Sperm Component</topic><topic>Female</topic><topic>Fertility</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Transfer Techniques</topic><topic>Genetics of the immune response</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - immunology</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Phagocytes - cytology</topic><topic>Plasmids - immunology</topic><topic>Spermatozoa - metabolism</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes, Cytotoxic - cytology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WU, Y.W.</creatorcontrib><creatorcontrib>CHEN, D.H.</creatorcontrib><creatorcontrib>MIAO, S. Y.</creatorcontrib><creatorcontrib>WANG, L.F.</creatorcontrib><creatorcontrib>ZONG, S. D.</creatorcontrib><creatorcontrib>KOIDE, S.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WU, Y.W.</au><au>CHEN, D.H.</au><au>MIAO, S. Y.</au><au>WANG, L.F.</au><au>ZONG, S. D.</au><au>KOIDE, S.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1)</atitle><jtitle>Archives of andrology</jtitle><addtitle>Arch Androl</addtitle><date>1999</date><risdate>1999</risdate><volume>42</volume><issue>3</issue><spage>127</spage><epage>136</epage><pages>127-136</pages><issn>0955-3002</issn><issn>0148-5016</issn><eissn>1362-3095</eissn><eissn>1521-0375</eissn><coden>ARANDR</coden><abstract>The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambdagt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female mice were inoculated intramuscularly with the plasmid DNA and the expression of HSD-1 was determined. HSD-1 mRNAs were detected in myocytes and endomysial connective tissue cells of the quadriceps muscle by in situ hybridization. Spleen of inoculated animals contained an increased number of cytotoxic T lymphocytes, phagocytes, and plasma cells. Fertility of the treated animals was not affected. Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response.</abstract><cop>Philadelphia, PA</cop><pub>Informa UK Ltd</pub><pmid>10407643</pmid><doi>10.1080/014850199262788</doi><tpages>10</tpages></addata></record> |
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| ispartof | Archives of andrology, 1999, Vol.42 (3), p.127-136 |
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| source | Taylor & Francis Online; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Animals Antigens, Surface Biological and medical sciences Connective Tissue - metabolism Cytotoxic t Lymphocytes Dna Vaccine Fertility Sperm Component Female Fertility Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Transfer Techniques Genetics of the immune response Humans Immunobiology In Situ Hybridization Male Membrane Proteins - biosynthesis Membrane Proteins - genetics Membrane Proteins - immunology Mice Molecular Sequence Data Muscle, Skeletal - metabolism Phagocytes - cytology Plasmids - immunology Spermatozoa - metabolism Spleen - immunology T-Lymphocytes, Cytotoxic - cytology T-Lymphocytes, Cytotoxic - immunology Transcription, Genetic |
| title | ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1) |
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