Deregulation of Aspartokinase by Single Nucleotide Exchange Leads to Global Flux Rearrangement in the Central Metabolism of Corynebacterium glutamicum

The wild-type Corynebacterium glutamicum ATTC 13032 and Corynebacterium glutamicum ATTC 13032 lysC S301Y, exhibiting a deregulated aspartokinase, were compared concerning growth, lysine production, and intracellular carbon fluxes. Both strains differ by only one single nucleotide over the whole geno...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006-08, Vol.16 (8), p.1174-1179
Hauptverfasser:	Kim, H.M. (Saarland University, Im Stadtwald, Saarbrucken, Germany), Heinzle, Elmar (Saarland University, Im Stadtwald, Saarbrucken, Germany), Wittmann, Christoph (Saarland University, Im Stadtwald, Saarbrucken, Germany), E-mail: c.wittmann@mx.uni-saarland.de
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology ESPECTROMETRIA DE MASAS Fundamental and applied biological sciences. Psychology isotopomer modeling LISINA lysC LYSINE MASS SPECTROMETRY metabolic flux analysis SPECTROMETRIE DE MASSE
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Zusammenfassung:	The wild-type Corynebacterium glutamicum ATTC 13032 and Corynebacterium glutamicum ATTC 13032 lysC S301Y, exhibiting a deregulated aspartokinase, were compared concerning growth, lysine production, and intracellular carbon fluxes. Both strains differ by only one single nucleotide over the whole genome. In comparison to the wild-type, the mutant showed significant production of lysine with a molar yield of 0.087 mol (mol glucose-¹) whereas the biomass yield was reduced. The deregulation of aspartokinase further led to a global rearrangement of carbon flux throughout the whole central metabolism.
ISSN:	1017-7825