Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms
A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric pro...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2006-02, Vol.72 (2), p.1180-1189 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1189 |
|---|---|
| container_issue | 2 |
| container_start_page | 1180 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 72 |
| creator | Mazzeo, Maria Fiorella Sorrentino, Alida Gaita, Marcello Cacace, Giuseppina Di Stasio, Michele Facchiano, Angelo Comi, Giuseppe Malorni, Antonio Siciliano, Rosa Anna |
| description | A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria. |
| doi_str_mv | 10.1128/aem.72.2.1180-1189.2006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_17492583</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>985978541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c549t-7aea3f3827b2a99cdda42202f61d8fe8fb3d9671c9a48ff1aef849ad631803763</originalsourceid><addsrcrecordid>eNpdkk1vEzEQhi0EoqHwF6hBgtsGf-x67QtS6AdUSsSh7dma7NqJq107tTe05cRPx9tEBLiMbfmZ1zPzGqETSqaUMvkJTD-t2ZTlkyRFDmrKCBHP0IQSJYuKc_EcTQhRqmCsJEfoVUq3hJCSCPkSHVFRCipENUG_FjBE91DMUnJpMC2eQzIRn5kU4mZwwePL4N1PGLfFtesNDhZfdG61HvACUsJXG9MMMfRmiI_YhoiHtcFnLjXR9c4_5T2lhNAWX0L0Bi9cE0OIK_Au9ek1emGhS-bNfj1GNxfn16ffivn3r5ens3nRVKUaihoMcMslq5cMlGraFkrGCLOCttIaaZe8VaKmjYJSWkvBWFkqaAXPA-K14Mfo8053s132pm2MHyJ0epPLhPioAzj97413a70KPzTliqlKZYGPe4EY7rYmDbrPXZquA2_CNmmqaiEVrTL4_j_wNmyjz81pRipVy5KOavUOyrNIKRr7pxJK9Gixnp0vdM0006PFY1B6tDhnvv27kUPe3tMMfNgDkBrobATfuHTg6lKxSvLMvdtx6-zmvYtGQ-p1_lmHZzNzsmMsBA2rmHVurhihnFBSMaEo_w1PLccg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205978419</pqid></control><display><type>article</type><title>Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Mazzeo, Maria Fiorella ; Sorrentino, Alida ; Gaita, Marcello ; Cacace, Giuseppina ; Di Stasio, Michele ; Facchiano, Angelo ; Comi, Giuseppe ; Malorni, Antonio ; Siciliano, Rosa Anna</creator><creatorcontrib>Mazzeo, Maria Fiorella ; Sorrentino, Alida ; Gaita, Marcello ; Cacace, Giuseppina ; Di Stasio, Michele ; Facchiano, Angelo ; Comi, Giuseppe ; Malorni, Antonio ; Siciliano, Rosa Anna</creatorcontrib><description>A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.72.2.1180-1189.2006</identifier><identifier>PMID: 16461665</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacteria - chemistry ; Bacteria - classification ; Bacteria - isolation & purification ; Bacteria - pathogenicity ; bacterial contamination ; Bacterial Proteins - isolation & purification ; Bacteriological Techniques - standards ; Biological and medical sciences ; Databases, Factual ; Escherichia coli ; Escherichia coli O157 - chemistry ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157:H7 ; food contamination ; Food industries ; Food Microbiology ; food pathogens ; Fundamental and applied biological sciences. Psychology ; ionization ; Lasers ; Mass spectrometry ; matrix-assisted laser desorption-time of flight mass spectrometry ; Microbiology ; pathogen identification ; Pathogens ; Sorption ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</subject><ispartof>Applied and Environmental Microbiology, 2006-02, Vol.72 (2), p.1180-1189</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Feb 2006</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-7aea3f3827b2a99cdda42202f61d8fe8fb3d9671c9a48ff1aef849ad631803763</citedby><cites>FETCH-LOGICAL-c549t-7aea3f3827b2a99cdda42202f61d8fe8fb3d9671c9a48ff1aef849ad631803763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1392959/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1392959/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17492583$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16461665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazzeo, Maria Fiorella</creatorcontrib><creatorcontrib>Sorrentino, Alida</creatorcontrib><creatorcontrib>Gaita, Marcello</creatorcontrib><creatorcontrib>Cacace, Giuseppina</creatorcontrib><creatorcontrib>Di Stasio, Michele</creatorcontrib><creatorcontrib>Facchiano, Angelo</creatorcontrib><creatorcontrib>Comi, Giuseppe</creatorcontrib><creatorcontrib>Malorni, Antonio</creatorcontrib><creatorcontrib>Siciliano, Rosa Anna</creatorcontrib><title>Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.</description><subject>Bacteria</subject><subject>Bacteria - chemistry</subject><subject>Bacteria - classification</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - pathogenicity</subject><subject>bacterial contamination</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacteriological Techniques - standards</subject><subject>Biological and medical sciences</subject><subject>Databases, Factual</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - chemistry</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157:H7</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ionization</subject><subject>Lasers</subject><subject>Mass spectrometry</subject><subject>matrix-assisted laser desorption-time of flight mass spectrometry</subject><subject>Microbiology</subject><subject>pathogen identification</subject><subject>Pathogens</subject><subject>Sorption</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkk1vEzEQhi0EoqHwF6hBgtsGf-x67QtS6AdUSsSh7dma7NqJq107tTe05cRPx9tEBLiMbfmZ1zPzGqETSqaUMvkJTD-t2ZTlkyRFDmrKCBHP0IQSJYuKc_EcTQhRqmCsJEfoVUq3hJCSCPkSHVFRCipENUG_FjBE91DMUnJpMC2eQzIRn5kU4mZwwePL4N1PGLfFtesNDhZfdG61HvACUsJXG9MMMfRmiI_YhoiHtcFnLjXR9c4_5T2lhNAWX0L0Bi9cE0OIK_Au9ek1emGhS-bNfj1GNxfn16ffivn3r5ens3nRVKUaihoMcMslq5cMlGraFkrGCLOCttIaaZe8VaKmjYJSWkvBWFkqaAXPA-K14Mfo8053s132pm2MHyJ0epPLhPioAzj97413a70KPzTliqlKZYGPe4EY7rYmDbrPXZquA2_CNmmqaiEVrTL4_j_wNmyjz81pRipVy5KOavUOyrNIKRr7pxJK9Gixnp0vdM0006PFY1B6tDhnvv27kUPe3tMMfNgDkBrobATfuHTg6lKxSvLMvdtx6-zmvYtGQ-p1_lmHZzNzsmMsBA2rmHVurhihnFBSMaEo_w1PLccg</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Mazzeo, Maria Fiorella</creator><creator>Sorrentino, Alida</creator><creator>Gaita, Marcello</creator><creator>Cacace, Giuseppina</creator><creator>Di Stasio, Michele</creator><creator>Facchiano, Angelo</creator><creator>Comi, Giuseppe</creator><creator>Malorni, Antonio</creator><creator>Siciliano, Rosa Anna</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20060201</creationdate><title>Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms</title><author>Mazzeo, Maria Fiorella ; Sorrentino, Alida ; Gaita, Marcello ; Cacace, Giuseppina ; Di Stasio, Michele ; Facchiano, Angelo ; Comi, Giuseppe ; Malorni, Antonio ; Siciliano, Rosa Anna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-7aea3f3827b2a99cdda42202f61d8fe8fb3d9671c9a48ff1aef849ad631803763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteria</topic><topic>Bacteria - chemistry</topic><topic>Bacteria - classification</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - pathogenicity</topic><topic>bacterial contamination</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacteriological Techniques - standards</topic><topic>Biological and medical sciences</topic><topic>Databases, Factual</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157 - chemistry</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157:H7</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ionization</topic><topic>Lasers</topic><topic>Mass spectrometry</topic><topic>matrix-assisted laser desorption-time of flight mass spectrometry</topic><topic>Microbiology</topic><topic>pathogen identification</topic><topic>Pathogens</topic><topic>Sorption</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazzeo, Maria Fiorella</creatorcontrib><creatorcontrib>Sorrentino, Alida</creatorcontrib><creatorcontrib>Gaita, Marcello</creatorcontrib><creatorcontrib>Cacace, Giuseppina</creatorcontrib><creatorcontrib>Di Stasio, Michele</creatorcontrib><creatorcontrib>Facchiano, Angelo</creatorcontrib><creatorcontrib>Comi, Giuseppe</creatorcontrib><creatorcontrib>Malorni, Antonio</creatorcontrib><creatorcontrib>Siciliano, Rosa Anna</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazzeo, Maria Fiorella</au><au>Sorrentino, Alida</au><au>Gaita, Marcello</au><au>Cacace, Giuseppina</au><au>Di Stasio, Michele</au><au>Facchiano, Angelo</au><au>Comi, Giuseppe</au><au>Malorni, Antonio</au><au>Siciliano, Rosa Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>72</volume><issue>2</issue><spage>1180</spage><epage>1189</epage><pages>1180-1189</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16461665</pmid><doi>10.1128/aem.72.2.1180-1189.2006</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2006-02, Vol.72 (2), p.1180-1189 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_17492583 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteria Bacteria - chemistry Bacteria - classification Bacteria - isolation & purification Bacteria - pathogenicity bacterial contamination Bacterial Proteins - isolation & purification Bacteriological Techniques - standards Biological and medical sciences Databases, Factual Escherichia coli Escherichia coli O157 - chemistry Escherichia coli O157 - isolation & purification Escherichia coli O157:H7 food contamination Food industries Food Microbiology food pathogens Fundamental and applied biological sciences. Psychology ionization Lasers Mass spectrometry matrix-assisted laser desorption-time of flight mass spectrometry Microbiology pathogen identification Pathogens Sorption Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards |
| title | Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T20%3A48%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Matrix-Assisted%20Laser%20Desorption%20Ionization-Time%20of%20Flight%20Mass%20Spectrometry%20for%20the%20Discrimination%20of%20Food-Borne%20Microorganisms&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Mazzeo,%20Maria%20Fiorella&rft.date=2006-02-01&rft.volume=72&rft.issue=2&rft.spage=1180&rft.epage=1189&rft.pages=1180-1189&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/aem.72.2.1180-1189.2006&rft_dat=%3Cproquest_pasca%3E985978541%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205978419&rft_id=info:pmid/16461665&rfr_iscdi=true |