Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus
The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open readin...
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Veröffentlicht in: | Journal of microbiology and biotechnology 2006-01, Vol.16 (1), p.32-36 |
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creator | Hwang, S.H. (Seoul National University, Seoul, Republic of Korea) Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) Lee, B.J. (Seoul National University, Seoul, Republic of Korea) Park, Y.S. (Seoul National University, Seoul, Republic of Korea) Choi, C.Y. (Seoul National University, Seoul, Republic of Korea) Han, J. (Inje University, Busan, Republic of Korea) Joo, H. (Inje University, Busan, Republic of Korea), E-mail: phyjoo@inje.ac.kr |
description | The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open reading frames (ORFs) from four microbial species, the ORF from Caulobacter crescentus showed an epoxide hydrolase activity. The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides. |
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(Seoul National University, Seoul, Republic of Korea) ; Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) ; Lee, B.J. (Seoul National University, Seoul, Republic of Korea) ; Park, Y.S. (Seoul National University, Seoul, Republic of Korea) ; Choi, C.Y. (Seoul National University, Seoul, Republic of Korea) ; Han, J. (Inje University, Busan, Republic of Korea) ; Joo, H. (Inje University, Busan, Republic of Korea), E-mail: phyjoo@inje.ac.kr</creator><creatorcontrib>Hwang, S.H. (Seoul National University, Seoul, Republic of Korea) ; Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) ; Lee, B.J. (Seoul National University, Seoul, Republic of Korea) ; Park, Y.S. (Seoul National University, Seoul, Republic of Korea) ; Choi, C.Y. (Seoul National University, Seoul, Republic of Korea) ; Han, J. (Inje University, Busan, Republic of Korea) ; Joo, H. 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The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; Caulobacter crescentus ; COMPOSE EPOXY ; COMPUESTOS EPOXY ; Epoxide hydrolase ; EPOXY COMPOUNDS ; Fundamental and applied biological sciences. 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The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Caulobacter crescentus</subject><subject>COMPOSE EPOXY</subject><subject>COMPUESTOS EPOXY</subject><subject>Epoxide hydrolase</subject><subject>EPOXY COMPOUNDS</subject><subject>Fundamental and applied biological sciences. 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(Seoul National University, Seoul, Republic of Korea)</creator><creator>Choi, C.Y. (Seoul National University, Seoul, Republic of Korea)</creator><creator>Han, J. (Inje University, Busan, Republic of Korea)</creator><creator>Joo, H. (Inje University, Busan, Republic of Korea), E-mail: phyjoo@inje.ac.kr</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>200601</creationdate><title>Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus</title><author>Hwang, S.H. (Seoul National University, Seoul, Republic of Korea) ; Hyun, H.J. (Seoul National University, Seoul, Republic of Korea) ; Lee, B.J. (Seoul National University, Seoul, Republic of Korea) ; Park, Y.S. (Seoul National University, Seoul, Republic of Korea) ; Choi, C.Y. (Seoul National University, Seoul, Republic of Korea) ; Han, J. (Inje University, Busan, Republic of Korea) ; Joo, H. 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(Seoul National University, Seoul, Republic of Korea)</au><au>Park, Y.S. (Seoul National University, Seoul, Republic of Korea)</au><au>Choi, C.Y. (Seoul National University, Seoul, Republic of Korea)</au><au>Han, J. (Inje University, Busan, Republic of Korea)</au><au>Joo, H. (Inje University, Busan, Republic of Korea), E-mail: phyjoo@inje.ac.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><date>2006-01</date><risdate>2006</risdate><volume>16</volume><issue>1</issue><spage>32</spage><epage>36</epage><pages>32-36</pages><issn>1017-7825</issn><abstract>The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open reading frames (ORFs) from four microbial species, the ORF from Caulobacter crescentus showed an epoxide hydrolase activity. The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><tpages>5</tpages></addata></record> |
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subjects | Biological and medical sciences Biotechnology Caulobacter crescentus COMPOSE EPOXY COMPUESTOS EPOXY Epoxide hydrolase EPOXY COMPOUNDS Fundamental and applied biological sciences. Psychology indene oxide kinetic resolution styrene oxide |
title | Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus |
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