Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus

The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open readin...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006-01, Vol.16 (1), p.32-36
Hauptverfasser:	Hwang, S.H. (Seoul National University, Seoul, Republic of Korea), Hyun, H.J. (Seoul National University, Seoul, Republic of Korea), Lee, B.J. (Seoul National University, Seoul, Republic of Korea), Park, Y.S. (Seoul National University, Seoul, Republic of Korea), Choi, C.Y. (Seoul National University, Seoul, Republic of Korea), Han, J. (Inje University, Busan, Republic of Korea), Joo, H. (Inje University, Busan, Republic of Korea), E-mail: phyjoo@inje.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Caulobacter crescentus COMPOSE EPOXY COMPUESTOS EPOXY Epoxide hydrolase EPOXY COMPOUNDS Fundamental and applied biological sciences. Psychology indene oxide kinetic resolution styrene oxide
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Zusammenfassung:	The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open reading frames (ORFs) from four microbial species, the ORF from Caulobacter crescentus showed an epoxide hydrolase activity. The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides.
ISSN:	1017-7825