Novel Bacterial Mucinase, Glycosulfatase, Is Associated with Bacterial Vaginosis
The modifications to the vaginal habitat accompanying a change to vaginal flora in bacterial vaginosis (BV) are poorly understood. In this study enzymes involved in mucin degradation were measured, including a novel glycosulfatase assay. Women attending an emergency walk-in sexually transmitted dise...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2005-11, Vol.43 (11), p.5504-5508 |
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| creator | Roberton, Anthony M Wiggins, Rebecca Horner, Patrick J Greenwood, Rosemary Crowley, Theresa Fernandes, Arnold Berry, Monica Corfield, Anthony P |
| description | The modifications to the vaginal habitat accompanying a change to vaginal flora in bacterial vaginosis (BV) are poorly understood. In this study enzymes involved in mucin degradation were measured, including a novel glycosulfatase assay. Women attending an emergency walk-in sexually transmitted disease clinic were studied. One high vaginal swab (HVS) was used to prepare a gram-stained smear to determine BV status, using Ison and Hay's criteria, and a separate swab was used for the purposes of the assays. The median glycosulfatase activity was 8.5 (range, -1.2 to 31.9) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to 0.5 (range, -0.7 to 9.4) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P = |
| doi_str_mv | 10.1128/JCM.43.11.5504-5508.2005 |
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In this study enzymes involved in mucin degradation were measured, including a novel glycosulfatase assay. Women attending an emergency walk-in sexually transmitted disease clinic were studied. One high vaginal swab (HVS) was used to prepare a gram-stained smear to determine BV status, using Ison and Hay's criteria, and a separate swab was used for the purposes of the assays. The median glycosulfatase activity was 8.5 (range, -1.2 to 31.9) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to 0.5 (range, -0.7 to 9.4) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P = <0.001). The median glycoprotein sialidase activity was 29.2 (range, -17 to 190) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to -1.1 (range, -41 to 48) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P < 0.001). A rapid spot test for sialidase was positive in 22/24 patients with BV (sensitivity, 91.7%; 95% confidence interval [CI], 73 to 99%) and negative in 32/35 patients without BV (specificity, 91.4%; 95% CI, 76.9 to 98.2%) (P < 0.001). Glycosulfatase activity significantly correlated with both glycoprotein sialidase activity and the sialidase spot test (P = 0.006 and P < 0.001, respectively). The results are consistent with the hypothesis that the consortium of bacteria present in BV requires the ability to break down mucins in order to colonize the vagina and replace the normal lactobacilli.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.43.11.5504-5508.2005</identifier><identifier>PMID: 16272477</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Adolescent ; Adult ; Bacteria ; Bacteria - isolation & purification ; Bacteriology ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious diseases ; Lactobacillus ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Mucins - metabolism ; Neuraminidase - analysis ; Sulfatases - analysis ; Vagina - enzymology ; Vagina - microbiology ; Vaginosis, Bacterial - metabolism ; Vaginosis, Bacterial - microbiology</subject><ispartof>Journal of Clinical Microbiology, 2005-11, Vol.43 (11), p.5504-5508</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-be71c391f08d4d0b05af7a0e11c8ec2a7e012f1d3c3c82fcff9b12ba6dded2863</citedby><cites>FETCH-LOGICAL-c530t-be71c391f08d4d0b05af7a0e11c8ec2a7e012f1d3c3c82fcff9b12ba6dded2863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287821/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287821/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17256888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16272477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roberton, Anthony M</creatorcontrib><creatorcontrib>Wiggins, Rebecca</creatorcontrib><creatorcontrib>Horner, Patrick J</creatorcontrib><creatorcontrib>Greenwood, Rosemary</creatorcontrib><creatorcontrib>Crowley, Theresa</creatorcontrib><creatorcontrib>Fernandes, Arnold</creatorcontrib><creatorcontrib>Berry, Monica</creatorcontrib><creatorcontrib>Corfield, Anthony P</creatorcontrib><title>Novel Bacterial Mucinase, Glycosulfatase, Is Associated with Bacterial Vaginosis</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The modifications to the vaginal habitat accompanying a change to vaginal flora in bacterial vaginosis (BV) are poorly understood. In this study enzymes involved in mucin degradation were measured, including a novel glycosulfatase assay. Women attending an emergency walk-in sexually transmitted disease clinic were studied. One high vaginal swab (HVS) was used to prepare a gram-stained smear to determine BV status, using Ison and Hay's criteria, and a separate swab was used for the purposes of the assays. The median glycosulfatase activity was 8.5 (range, -1.2 to 31.9) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to 0.5 (range, -0.7 to 9.4) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P = <0.001). The median glycoprotein sialidase activity was 29.2 (range, -17 to 190) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to -1.1 (range, -41 to 48) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P < 0.001). A rapid spot test for sialidase was positive in 22/24 patients with BV (sensitivity, 91.7%; 95% confidence interval [CI], 73 to 99%) and negative in 32/35 patients without BV (specificity, 91.4%; 95% CI, 76.9 to 98.2%) (P < 0.001). Glycosulfatase activity significantly correlated with both glycoprotein sialidase activity and the sialidase spot test (P = 0.006 and P < 0.001, respectively). The results are consistent with the hypothesis that the consortium of bacteria present in BV requires the ability to break down mucins in order to colonize the vagina and replace the normal lactobacilli.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Bacteria</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Lactobacillus</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Mucins - metabolism</subject><subject>Neuraminidase - analysis</subject><subject>Sulfatases - analysis</subject><subject>Vagina - enzymology</subject><subject>Vagina - microbiology</subject><subject>Vaginosis, Bacterial - metabolism</subject><subject>Vaginosis, Bacterial - microbiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhSMEotvCX4AICU5kmbHj2LlUKqtSilpAgiJu1qxj77pK4hInrfrv8bIrupy4jMfy997YflmWI8wRmXr3aXE5L3nq50JAWaSi5gxAPMpmCLUqqgp-Ps5mALUoELk8yA5jvAbAshTiaXaAFZOslHKWff0cbm2bvycz2sFTm19OxvcU7dv8rL03IU6to_HP_jzmJzEG42m0TX7nx_We7AetfB-ij8-yJ47aaJ_v1qPs6sPp98XH4uLL2fni5KIwgsNYLK1Ew2t0oJqygSUIcpLAIhplDSNpAZnDhhtuFHPGuXqJbElV09iGqYofZcdb35tp2dnG2H4cqNU3g-9ouNeBvP73pPdrvQq3Ov2fVAyTwZudwRB-TTaOuvPR2Lal3oYp6krJSpZQ_RfEmkupUCRQbUEzhBgH6_7eBkFvctMpN13y1OtNbpui9Ca3JH2x_5oH4S6oBLzeARQNtW6g3vj4wEkmKqVU4l5tubVfre_8YDXFTl-bbm9ugl5uIUdB02pIRlffGCAHBK6YqPlvLf23kg</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Roberton, Anthony M</creator><creator>Wiggins, Rebecca</creator><creator>Horner, Patrick J</creator><creator>Greenwood, Rosemary</creator><creator>Crowley, Theresa</creator><creator>Fernandes, Arnold</creator><creator>Berry, Monica</creator><creator>Corfield, Anthony P</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051101</creationdate><title>Novel Bacterial Mucinase, Glycosulfatase, Is Associated with Bacterial Vaginosis</title><author>Roberton, Anthony M ; Wiggins, Rebecca ; Horner, Patrick J ; Greenwood, Rosemary ; Crowley, Theresa ; Fernandes, Arnold ; Berry, Monica ; Corfield, Anthony P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-be71c391f08d4d0b05af7a0e11c8ec2a7e012f1d3c3c82fcff9b12ba6dded2863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacteria</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Lactobacillus</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Mucins - metabolism</topic><topic>Neuraminidase - analysis</topic><topic>Sulfatases - analysis</topic><topic>Vagina - enzymology</topic><topic>Vagina - microbiology</topic><topic>Vaginosis, Bacterial - metabolism</topic><topic>Vaginosis, Bacterial - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roberton, Anthony M</creatorcontrib><creatorcontrib>Wiggins, Rebecca</creatorcontrib><creatorcontrib>Horner, Patrick J</creatorcontrib><creatorcontrib>Greenwood, Rosemary</creatorcontrib><creatorcontrib>Crowley, Theresa</creatorcontrib><creatorcontrib>Fernandes, Arnold</creatorcontrib><creatorcontrib>Berry, Monica</creatorcontrib><creatorcontrib>Corfield, Anthony P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roberton, Anthony M</au><au>Wiggins, Rebecca</au><au>Horner, Patrick J</au><au>Greenwood, Rosemary</au><au>Crowley, Theresa</au><au>Fernandes, Arnold</au><au>Berry, Monica</au><au>Corfield, Anthony P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel Bacterial Mucinase, Glycosulfatase, Is Associated with Bacterial Vaginosis</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>43</volume><issue>11</issue><spage>5504</spage><epage>5508</epage><pages>5504-5508</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>The modifications to the vaginal habitat accompanying a change to vaginal flora in bacterial vaginosis (BV) are poorly understood. In this study enzymes involved in mucin degradation were measured, including a novel glycosulfatase assay. Women attending an emergency walk-in sexually transmitted disease clinic were studied. One high vaginal swab (HVS) was used to prepare a gram-stained smear to determine BV status, using Ison and Hay's criteria, and a separate swab was used for the purposes of the assays. The median glycosulfatase activity was 8.5 (range, -1.2 to 31.9) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to 0.5 (range, -0.7 to 9.4) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P = <0.001). The median glycoprotein sialidase activity was 29.2 (range, -17 to 190) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients with BV compared to -1.1 (range, -41 to 48) nmol h⁻¹ 1.5 ml⁻¹ of HVS suspension in patients without BV (P < 0.001). A rapid spot test for sialidase was positive in 22/24 patients with BV (sensitivity, 91.7%; 95% confidence interval [CI], 73 to 99%) and negative in 32/35 patients without BV (specificity, 91.4%; 95% CI, 76.9 to 98.2%) (P < 0.001). Glycosulfatase activity significantly correlated with both glycoprotein sialidase activity and the sialidase spot test (P = 0.006 and P < 0.001, respectively). The results are consistent with the hypothesis that the consortium of bacteria present in BV requires the ability to break down mucins in order to colonize the vagina and replace the normal lactobacilli.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16272477</pmid><doi>10.1128/JCM.43.11.5504-5508.2005</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Bacteria Bacteria - isolation & purification Bacteriology Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Humans Infectious diseases Lactobacillus Medical sciences Microbiology Middle Aged Miscellaneous Mucins - metabolism Neuraminidase - analysis Sulfatases - analysis Vagina - enzymology Vagina - microbiology Vaginosis, Bacterial - metabolism Vaginosis, Bacterial - microbiology |
| title | Novel Bacterial Mucinase, Glycosulfatase, Is Associated with Bacterial Vaginosis |
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