Identification of the sprU Gene Encoding an Additional sprT Homologous Trypsin-Type Protease in Streptomyces griseus
Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology (79% identity, 88% similarity) with the SGT protease, which has been reported as a bacteria...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2005-10, Vol.15 (5), p.1125-1129 |
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| creator | Yang, H.Y. (Myongji University, Yongin, Republic of Korea) Choi, S.S. (Myongji University, Yongin, Republic of Korea) Chi, W.J. (Myongji University, Yongin, Republic of Korea) Kim, J.H. (Myongji University, Yongin, Republic of Korea) Kang, D.K. (Easy Bio System Inc., Cheonan, Republic of Korea) Chun, J.S. (Chungbuk National University, Cheongju, Republic of Korea) Kang, S.S. (Chungbuk National University, Cheongju, Republic of Korea) Hong, S.K. (Myongji University, Yongin, Republic of Korea), E-mail: skhong@mju.ac.kr |
| description | Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology (79% identity, 88% similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. |
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(Myongji University, Yongin, Republic of Korea) ; Choi, S.S. (Myongji University, Yongin, Republic of Korea) ; Chi, W.J. (Myongji University, Yongin, Republic of Korea) ; Kim, J.H. (Myongji University, Yongin, Republic of Korea) ; Kang, D.K. (Easy Bio System Inc., Cheonan, Republic of Korea) ; Chun, J.S. (Chungbuk National University, Cheongju, Republic of Korea) ; Kang, S.S. (Chungbuk National University, Cheongju, Republic of Korea) ; Hong, S.K. (Myongji University, Yongin, Republic of Korea), E-mail: skhong@mju.ac.kr</creator><creatorcontrib>Yang, H.Y. (Myongji University, Yongin, Republic of Korea) ; Choi, S.S. (Myongji University, Yongin, Republic of Korea) ; Chi, W.J. (Myongji University, Yongin, Republic of Korea) ; Kim, J.H. (Myongji University, Yongin, Republic of Korea) ; Kang, D.K. (Easy Bio System Inc., Cheonan, Republic of Korea) ; Chun, J.S. (Chungbuk National University, Cheongju, Republic of Korea) ; Kang, S.S. 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The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>bacterial trypsin ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. 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The SprU protein shows a high homology (79% identity, 88% similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13.</description><subject>bacterial trypsin</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>SprT</subject><subject>SprU</subject><subject>STREPTOMYCES GRISEUS</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotkDFrwzAUhD200DTtTyho6WiwJFuyxxDSJDTQ0jqzeZGeUhVbMpIy-N_XIeWGG-7jOO4uW9CCylzWrHrIHmP8LQpBWS0WWdprdMkaqyBZ74g3JP0giWM4ki06JBunvLbuTMCRldb2SkF_BVqy84Pv_dlfImnDNEbr8nYakXwGnxAiEuvIdwo4Jj9MCiM5BxvxEp-yewN9xOd_X2bHt0273uWHj-1-vTrkhhUy5chRcN5URvJTJSWvuSw0L5ksmUImlBHUCK5KRRU2qgZowICq9EmL6jSLL7PXW-8IUUFvAjhlYzcGO0CYOioZl5KymXu5cQZ8B9eR3fsXmz8qaMXn_A9D3mHe</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Yang, H.Y. (Myongji University, Yongin, Republic of Korea)</creator><creator>Choi, S.S. (Myongji University, Yongin, Republic of Korea)</creator><creator>Chi, W.J. (Myongji University, Yongin, Republic of Korea)</creator><creator>Kim, J.H. 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(Easy Bio System Inc., Cheonan, Republic of Korea) ; Chun, J.S. (Chungbuk National University, Cheongju, Republic of Korea) ; Kang, S.S. (Chungbuk National University, Cheongju, Republic of Korea) ; Hong, S.K. (Myongji University, Yongin, Republic of Korea), E-mail: skhong@mju.ac.kr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f207t-e3e63395f73b57738370d342742ce26cf61f63c4c1ce9c8aa9afac5dbd65b5b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>bacterial trypsin</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>SprT</topic><topic>SprU</topic><topic>STREPTOMYCES GRISEUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, H.Y. (Myongji University, Yongin, Republic of Korea)</creatorcontrib><creatorcontrib>Choi, S.S. 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(Myongji University, Yongin, Republic of Korea)</au><au>Chi, W.J. (Myongji University, Yongin, Republic of Korea)</au><au>Kim, J.H. (Myongji University, Yongin, Republic of Korea)</au><au>Kang, D.K. (Easy Bio System Inc., Cheonan, Republic of Korea)</au><au>Chun, J.S. (Chungbuk National University, Cheongju, Republic of Korea)</au><au>Kang, S.S. (Chungbuk National University, Cheongju, Republic of Korea)</au><au>Hong, S.K. 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The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><tpages>5</tpages></addata></record> |
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| subjects | bacterial trypsin Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology SprT SprU STREPTOMYCES GRISEUS |
| title | Identification of the sprU Gene Encoding an Additional sprT Homologous Trypsin-Type Protease in Streptomyces griseus |
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