Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm

An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter....

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Veröffentlicht in:Journal of microbiology and biotechnology 2005-06, Vol.15 (3), p.466-471
Hauptverfasser: Chen, Yin (Chinese Academy of Agricultural Sciences, Zhenjiang, China), Zhu, Zhongze (Chinese Academy of Agricultural Sciences, Zhenjiang, China), Lin, Xu' Ai (Chinese Academy of Agricultural Sciences, Zhenjiang, China), Yi, Yongzhu (Chinese Academy of Agricultural Sciences, Zhenjiang, China), Zhang, Zhifang (Chinese Academy of Agricultural Sciences, Zhenjiang, China), E-mail: zhifangzhang@yahoo.com, Shen, Guifang (Chinese Academy of Agricultural Sciences, Beijing, China)
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container_title Journal of microbiology and biotechnology
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creator Chen, Yin (Chinese Academy of Agricultural Sciences, Zhenjiang, China)
Zhu, Zhongze (Chinese Academy of Agricultural Sciences, Zhenjiang, China)
Lin, Xu' Ai (Chinese Academy of Agricultural Sciences, Zhenjiang, China)
Yi, Yongzhu (Chinese Academy of Agricultural Sciences, Zhenjiang, China)
Zhang, Zhifang (Chinese Academy of Agricultural Sciences, Zhenjiang, China), E-mail: zhifangzhang@yahoo.com
Shen, Guifang (Chinese Academy of Agricultural Sciences, Beijing, China)
description An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early 5∨th instar larvae of silkworm were infected with the recombinant virus.
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subjects appA
BACULOVIRIDAE
Biological and medical sciences
Biotechnology
ESCHERICHIA COLI
FITASA
Fundamental and applied biological sciences. Psychology
GUSANO DE SEDA
PHYTASE
SILKWORMS
VER A SOIE
title Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm
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