Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm
An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter....
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Veröffentlicht in: | Journal of microbiology and biotechnology 2005-06, Vol.15 (3), p.466-471 |
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container_title | Journal of microbiology and biotechnology |
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creator | Chen, Yin (Chinese Academy of Agricultural Sciences, Zhenjiang, China) Zhu, Zhongze (Chinese Academy of Agricultural Sciences, Zhenjiang, China) Lin, Xu' Ai (Chinese Academy of Agricultural Sciences, Zhenjiang, China) Yi, Yongzhu (Chinese Academy of Agricultural Sciences, Zhenjiang, China) Zhang, Zhifang (Chinese Academy of Agricultural Sciences, Zhenjiang, China), E-mail: zhifangzhang@yahoo.com Shen, Guifang (Chinese Academy of Agricultural Sciences, Beijing, China) |
description | An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early 5∨th instar larvae of silkworm were infected with the recombinant virus. |
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The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early 5∨th instar larvae of silkworm were infected with the recombinant virus.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>appA ; BACULOVIRIDAE ; Biological and medical sciences ; Biotechnology ; ESCHERICHIA COLI ; FITASA ; Fundamental and applied biological sciences. 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The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early 5∨th instar larvae of silkworm were infected with the recombinant virus.</description><subject>appA</subject><subject>BACULOVIRIDAE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ESCHERICHIA COLI</subject><subject>FITASA</subject><subject>Fundamental and applied biological sciences. 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subjects | appA BACULOVIRIDAE Biological and medical sciences Biotechnology ESCHERICHIA COLI FITASA Fundamental and applied biological sciences. Psychology GUSANO DE SEDA PHYTASE SILKWORMS VER A SOIE |
title | Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm |
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