Optimization of Cry3A yields in Bacillus thuringiensis by use of sporulation-dependent promoters in combination with the STAB-SD mRNA sequence

The insecticidal activity of Bacillus thuringiensis strains toxic to coleopterous insects is due to Cry3 proteins assembled into small rectangular crystals. Toxin synthesis in these strains is dependent primarily upon a promoter that is active in the stationary phase and a STAB-SD sequence that stab...

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Veröffentlicht in:	Applied and Environmental Microbiology 1998-10, Vol.64 (10), p.3932-3938
Hauptverfasser:	Park, H.W. (University of California, Riverside, CA.), Ge, B, Bauer, L.S, Federici, B.A
Format:	Artikel
Sprache:	eng
Schlagworte:	BACILLUS THURINGIENSIS Bacillus thuringiensis - genetics Bacillus thuringiensis - growth & development Bacillus thuringiensis - physiology BACILLUS THURINGIENSIS SUBSP. ISRAELENSI BACILLUS THURINGIENSIS SUBSP. MORRISONI Bacteria Bacterial Proteins - analysis Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Proteins - ultrastructure BACTERIAL TOXINS Bacterial Toxins - biosynthesis Bacterial Toxins - genetics Biological and medical sciences Biological control Biotechnology Cell Division Chrysomela scripta Control CRYSTAL PROTEINS Culture Media Endotoxins - analysis Endotoxins - biosynthesis Endotoxins - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Genes Genetic engineering Genetic technics Hemolysin Proteins INSECTICIDAL PROPERTIES INSECTICIDAS INSECTICIDE INSECTICIDES Methods. Procedures. Technologies Microscopy, Electron Modification of gene expression level Physiology and Biotechnology Phytopathology. Animal pests. Plant and forest protection Plasmids Promoter Regions, Genetic PROTEINAS PROTEINE PROTEINS Protozoa. Invertebrates Ribonucleic acid Ribosomes - metabolism RNA RNA, Bacterial - genetics RNA, Messenger - genetics Spores, Bacterial - genetics Spores, Bacterial - physiology TOXINAS BACTERIANAS TOXINE BACTERIENNE Transcription, Genetic
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