Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase
Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The tr...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2005-02, Vol.15 (1), p.99-104 |
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| creator | Kim, M.J. (Pusan National University, Busan, Republic of Korea) Park, H.E. (Pusan National University, Busan, Republic of Korea) Sung, H.K. (Pusan National University, Busan, Republic of Korea) Park, T.H. (Pusan National University, Busan, Republic of Korea) Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr |
| description | Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O-α-D-galactosyl-(1→2)-β-D-fructofuranoside. |
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(Pusan National University, Busan, Republic of Korea) ; Park, H.E. (Pusan National University, Busan, Republic of Korea) ; Sung, H.K. (Pusan National University, Busan, Republic of Korea) ; Park, T.H. (Pusan National University, Busan, Republic of Korea) ; Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr</creator><creatorcontrib>Kim, M.J. (Pusan National University, Busan, Republic of Korea) ; Park, H.E. (Pusan National University, Busan, Republic of Korea) ; Sung, H.K. (Pusan National University, Busan, Republic of Korea) ; Park, T.H. (Pusan National University, Busan, Republic of Korea) ; Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr</creatorcontrib><description>Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O-α-D-galactosyl-(1→2)-β-D-fructofuranoside.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; hetero-oligosaccharide ; Levansucrase ; SACCHAROSE ; SUCROSA ; SUCROSE ; transfructosylation</subject><ispartof>Journal of microbiology and biotechnology, 2005-02, Vol.15 (1), p.99-104</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16589460$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, M.J. (Pusan National University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Park, H.E. (Pusan National University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Sung, H.K. (Pusan National University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Park, T.H. (Pusan National University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr</creatorcontrib><title>Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase</title><title>Journal of microbiology and biotechnology</title><description>Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O-α-D-galactosyl-(1→2)-β-D-fructofuranoside.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hetero-oligosaccharide</subject><subject>Levansucrase</subject><subject>SACCHAROSE</subject><subject>SUCROSA</subject><subject>SUCROSE</subject><subject>transfructosylation</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNot0E1LxDAQBuAcFFxXf4KQi8dCJm2T9LgUv7CLIOu5TPOhkX4sSSvUX290PQ0Dz_vCzBnZAAOZScXLC3IZ4ydjArgSG9Lu9Oynke6t_sDRx4FOjh4CjtGFRc9TXHv8AzXO2K_f1tBupXuvw9Shnm3wy0B7tF8p7KYwpCBtfre46IDRXpFzh3201_9zS97u7w71Y9a8PDzVuyZznMk54-hKrAyTHJgE1zHhrIAclGFKKKk6zSqlDC9YUs4WADznlbLSatOhEfmW3J56jxg19i5doH1sj8EPGNYWRKmqQrDkbk7O4dTie0jm-ZWndzAooRT5DxeyWqc</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Kim, M.J. (Pusan National University, Busan, Republic of Korea)</creator><creator>Park, H.E. (Pusan National University, Busan, Republic of Korea)</creator><creator>Sung, H.K. (Pusan National University, Busan, Republic of Korea)</creator><creator>Park, T.H. (Pusan National University, Busan, Republic of Korea)</creator><creator>Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>20050201</creationdate><title>Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase</title><author>Kim, M.J. (Pusan National University, Busan, Republic of Korea) ; Park, H.E. (Pusan National University, Busan, Republic of Korea) ; Sung, H.K. (Pusan National University, Busan, Republic of Korea) ; Park, T.H. (Pusan National University, Busan, Republic of Korea) ; Cha, J.H. 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(Pusan National University, Busan, Republic of Korea), E-mail: jhcha@pusan.ac.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><date>2005-02-01</date><risdate>2005</risdate><volume>15</volume><issue>1</issue><spage>99</spage><epage>104</epage><pages>99-104</pages><issn>1017-7825</issn><abstract>Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O-α-D-galactosyl-(1→2)-β-D-fructofuranoside.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><tpages>6</tpages></addata></record> |
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| ispartof | Journal of microbiology and biotechnology, 2005-02, Vol.15 (1), p.99-104 |
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| subjects | Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology hetero-oligosaccharide Levansucrase SACCHAROSE SUCROSA SUCROSE transfructosylation |
| title | Action Mechanism of Transfructosylation Catalyzed by Microbacterium laevaniformans Levansucrase |
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