Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis
Meristem maintenance and differentiation is regulated by intercellular communication through receptor-like kinases (RLKs) in plants, but the underlying molecular mechanisms of RLK signaling remain largely unknown. A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK),...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-12, Vol.68 (12), p.2598-2606 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | HATTAN, Junichiro KANAMOTO, Hirosuke TAKEMURA, Miho YOKOTA, Akiho KOHCHI, Takayuki |
| description | Meristem maintenance and differentiation is regulated by intercellular communication through receptor-like kinases (RLKs) in plants, but the underlying molecular mechanisms of RLK signaling remain largely unknown. A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK), which is a typical meristematic RLK with leucine-rich repeats in Arabidopsis, was identified using a yeast two-hybrid assay and named IRK-interacting protein (IRKI). IRKI is a novel but highly conserved protein found in higher plants. The interaction between IRK and IRKI was confirmed by an in vitro pull-down assay and supported by their simultaneous expression in actively dividing cells in meristems. In the root tip, IRKI expression and localization visualized by green fluorescence protein (GFP) were observed in the quiescent center, initial cells, and immature stele cells. IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport. |
| doi_str_mv | 10.1271/bbb.68.2598 |
| format | Article |
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A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK), which is a typical meristematic RLK with leucine-rich repeats in Arabidopsis, was identified using a yeast two-hybrid assay and named IRK-interacting protein (IRKI). IRKI is a novel but highly conserved protein found in higher plants. The interaction between IRK and IRKI was confirmed by an in vitro pull-down assay and supported by their simultaneous expression in actively dividing cells in meristems. In the root tip, IRKI expression and localization visualized by green fluorescence protein (GFP) were observed in the quiescent center, initial cells, and immature stele cells. IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.68.2598</identifier><identifier>PMID: 15618632</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; Arabidopsis - cytology ; Arabidopsis Proteins - analysis ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; auxin ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell Proliferation ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant - drug effects ; Green Fluorescent Proteins ; Indoleacetic Acids - pharmacology ; initial cell ; meristem ; Meristem - chemistry ; Meristem - cytology ; Molecular Sequence Data ; Plant Roots - chemistry ; Protein Binding ; Protein Kinases - genetics ; Protein Kinases - metabolism ; receptor-like kinase ; Sequence Alignment ; Signal Transduction ; Transfection ; Two-Hybrid System Techniques</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2004-12, Vol.68 (12), p.2598-2606</ispartof><rights>2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2004</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-e0b56be21aa1a2d9d45deb1b5607920c0be0a5fb616433ecfee9a5349b2242a73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16571765$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15618632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HATTAN, Junichiro</creatorcontrib><creatorcontrib>KANAMOTO, Hirosuke</creatorcontrib><creatorcontrib>TAKEMURA, Miho</creatorcontrib><creatorcontrib>YOKOTA, Akiho</creatorcontrib><creatorcontrib>KOHCHI, Takayuki</creatorcontrib><title>Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Meristem maintenance and differentiation is regulated by intercellular communication through receptor-like kinases (RLKs) in plants, but the underlying molecular mechanisms of RLK signaling remain largely unknown. A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK), which is a typical meristematic RLK with leucine-rich repeats in Arabidopsis, was identified using a yeast two-hybrid assay and named IRK-interacting protein (IRKI). IRKI is a novel but highly conserved protein found in higher plants. The interaction between IRK and IRKI was confirmed by an in vitro pull-down assay and supported by their simultaneous expression in actively dividing cells in meristems. In the root tip, IRKI expression and localization visualized by green fluorescence protein (GFP) were observed in the quiescent center, initial cells, and immature stele cells. IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport.</description><subject>Amino Acid Sequence</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis Proteins - analysis</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>auxin</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Proliferation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Green Fluorescent Proteins</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>initial cell</subject><subject>meristem</subject><subject>Meristem - chemistry</subject><subject>Meristem - cytology</subject><subject>Molecular Sequence Data</subject><subject>Plant Roots - chemistry</subject><subject>Protein Binding</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>receptor-like kinase</subject><subject>Sequence Alignment</subject><subject>Signal Transduction</subject><subject>Transfection</subject><subject>Two-Hybrid System Techniques</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0UGL1DAUB_AiijuunrxLQNaLdMxrm7Q5DsO6O-yKMui5vKSvbpZOUpMWHT-Kn9YMM7ogngL__PJ45J9lL4EvoajhndZ6KZtlIVTzKFtAWdW5VFX9OFtwBTJvKgFn2bMY7zlPgYCn2RkICY0si0X264MfyMwDBra-w4BmomB_4mS9Y75n0x2x9X7y44BxZw3buHSfkHVf2afgJ7J_2ZYMjZMP7MY6jMQ22xt2-WMMFCN1LLkD2rh-8Cky5AwxdB3bej-x1WgNxcOkVUBtOz9GG59nT3ocIr04nefZl_eXn9fX-e3Hq816dZsbAc2UE9dCaioAEbDoVFeJjjSkkNeq4IZr4ih6LUFWZUmmJ1IoykrpoqgKrMvz7M1x7hj8t5ni1O5sWnAY0JGfYytrUKJUKsHX_8B7PweXdmuhqlQDwEtI6u1RmeBjDNS3Y7A7DPsWeHsorE2FtbJpD4Ul_eo0c9Y76h7sqaEELk4Ao8GhD-iMjQ9OihpqKZKTR2dd78MOv_swdO2E-_Tffx6V_9vgNxoZsoo</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>HATTAN, Junichiro</creator><creator>KANAMOTO, Hirosuke</creator><creator>TAKEMURA, Miho</creator><creator>YOKOTA, Akiho</creator><creator>KOHCHI, Takayuki</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis</title><author>HATTAN, Junichiro ; KANAMOTO, Hirosuke ; TAKEMURA, Miho ; YOKOTA, Akiho ; KOHCHI, Takayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-e0b56be21aa1a2d9d45deb1b5607920c0be0a5fb616433ecfee9a5349b2242a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis Proteins - analysis</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>auxin</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Proliferation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Green Fluorescent Proteins</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>initial cell</topic><topic>meristem</topic><topic>Meristem - chemistry</topic><topic>Meristem - cytology</topic><topic>Molecular Sequence Data</topic><topic>Plant Roots - chemistry</topic><topic>Protein Binding</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>receptor-like kinase</topic><topic>Sequence Alignment</topic><topic>Signal Transduction</topic><topic>Transfection</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HATTAN, Junichiro</creatorcontrib><creatorcontrib>KANAMOTO, Hirosuke</creatorcontrib><creatorcontrib>TAKEMURA, Miho</creatorcontrib><creatorcontrib>YOKOTA, Akiho</creatorcontrib><creatorcontrib>KOHCHI, Takayuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HATTAN, Junichiro</au><au>KANAMOTO, Hirosuke</au><au>TAKEMURA, Miho</au><au>YOKOTA, Akiho</au><au>KOHCHI, Takayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>68</volume><issue>12</issue><spage>2598</spage><epage>2606</epage><pages>2598-2606</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Meristem maintenance and differentiation is regulated by intercellular communication through receptor-like kinases (RLKs) in plants, but the underlying molecular mechanisms of RLK signaling remain largely unknown. A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK), which is a typical meristematic RLK with leucine-rich repeats in Arabidopsis, was identified using a yeast two-hybrid assay and named IRK-interacting protein (IRKI). IRKI is a novel but highly conserved protein found in higher plants. The interaction between IRK and IRKI was confirmed by an in vitro pull-down assay and supported by their simultaneous expression in actively dividing cells in meristems. In the root tip, IRKI expression and localization visualized by green fluorescence protein (GFP) were observed in the quiescent center, initial cells, and immature stele cells. IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15618632</pmid><doi>10.1271/bbb.68.2598</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Arabidopsis - cytology Arabidopsis Proteins - analysis Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana auxin Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Cell Proliferation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - drug effects Green Fluorescent Proteins Indoleacetic Acids - pharmacology initial cell meristem Meristem - chemistry Meristem - cytology Molecular Sequence Data Plant Roots - chemistry Protein Binding Protein Kinases - genetics Protein Kinases - metabolism receptor-like kinase Sequence Alignment Signal Transduction Transfection Two-Hybrid System Techniques |
| title | Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis |
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