Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies
Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embr...
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| Veröffentlicht in: | Canadian journal of forest research 1998-10, Vol.28 (10), p.1536-1545 |
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| creator | Hogberg, K.A Ekberg, I Norell, L Arnold, S. von |
| description | Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessity when dealing with a large number of genotypes. Proliferation and maturation were identified as major constraints. Clear differences among families were seen for proliferation and for maturation. For plant regeneration, differences among cell lines within families were considerable while no significant differences were found among families. Significant differences among male parents were obtained for proliferation and maturation. Our calculations show that by using an improved standard protocol, at least one third of the genotypes can be propagated via somatic embryogenesis. No clear relationship was found between embryogenic characters of selected parents based on progenies and corresponding parental breeding values for growth and phenology characters. Three years can be gained by using somatic embryogenesis instead of cuttings in a breeding programme. Maintained propagation ability by including cryopreservation in the breeding programme will provide a very useful link between breeding and mass propagation. The results are encouraging, but further improvement of the standard protocol is necessary. |
| doi_str_mv | 10.1139/x98-137 |
| format | Article |
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Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessity when dealing with a large number of genotypes. Proliferation and maturation were identified as major constraints. Clear differences among families were seen for proliferation and for maturation. For plant regeneration, differences among cell lines within families were considerable while no significant differences were found among families. Significant differences among male parents were obtained for proliferation and maturation. Our calculations show that by using an improved standard protocol, at least one third of the genotypes can be propagated via somatic embryogenesis. No clear relationship was found between embryogenic characters of selected parents based on progenies and corresponding parental breeding values for growth and phenology characters. Three years can be gained by using somatic embryogenesis instead of cuttings in a breeding programme. Maintained propagation ability by including cryopreservation in the breeding programme will provide a very useful link between breeding and mass propagation. The results are encouraging, but further improvement of the standard protocol is necessary.</description><identifier>ISSN: 0045-5067</identifier><identifier>EISSN: 1208-6037</identifier><identifier>DOI: 10.1139/x98-137</identifier><identifier>CODEN: CJFRAR</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>agricultural programs and projects ; Biological and medical sciences ; Biotechnology ; Breeding ; Calculations ; Case studies ; cryopreservation ; Cuttings ; embryo (plant) ; Embryology ; Embryonic growth stage ; Embryos ; Eukaryotic cell cultures ; Forestry ; Fundamental and applied biological sciences. Psychology ; genotype ; Genotypes ; height ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; maturation ; Methods. Procedures. Technologies ; micropropagation ; phenology ; Picea abies ; Pine trees ; Plant breeding ; Plant cells and fungal cells ; plant characteristics ; Plant production ; Plant propagation ; Plants (botany) ; regenerative ability ; seedlings ; seeds ; somatic embryogenesis ; Spruce ; Thawing ; tissue culture ; tree breeding ; Trees ; Vegetation ; yields</subject><ispartof>Canadian journal of forest research, 1998-10, Vol.28 (10), p.1536-1545</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright National Research Council of Canada Oct 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3517-c069f22476febd7e5bb50142812e93e0d3d794be26f793a95fa0fe4c76fe35ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1655432$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-28217$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Hogberg, K.A</creatorcontrib><creatorcontrib>Ekberg, I</creatorcontrib><creatorcontrib>Norell, L</creatorcontrib><creatorcontrib>Arnold, S. von</creatorcontrib><title>Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies</title><title>Canadian journal of forest research</title><addtitle>Revue canadienne de recherche forestière</addtitle><description>Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessity when dealing with a large number of genotypes. Proliferation and maturation were identified as major constraints. Clear differences among families were seen for proliferation and for maturation. For plant regeneration, differences among cell lines within families were considerable while no significant differences were found among families. Significant differences among male parents were obtained for proliferation and maturation. Our calculations show that by using an improved standard protocol, at least one third of the genotypes can be propagated via somatic embryogenesis. No clear relationship was found between embryogenic characters of selected parents based on progenies and corresponding parental breeding values for growth and phenology characters. Three years can be gained by using somatic embryogenesis instead of cuttings in a breeding programme. Maintained propagation ability by including cryopreservation in the breeding programme will provide a very useful link between breeding and mass propagation. The results are encouraging, but further improvement of the standard protocol is necessary.</description><subject>agricultural programs and projects</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Breeding</subject><subject>Calculations</subject><subject>Case studies</subject><subject>cryopreservation</subject><subject>Cuttings</subject><subject>embryo (plant)</subject><subject>Embryology</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Eukaryotic cell cultures</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genotype</subject><subject>Genotypes</subject><subject>height</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>maturation</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>phenology</subject><subject>Picea abies</subject><subject>Pine trees</subject><subject>Plant breeding</subject><subject>Plant cells and fungal cells</subject><subject>plant characteristics</subject><subject>Plant production</subject><subject>Plant propagation</subject><subject>Plants (botany)</subject><subject>regenerative ability</subject><subject>seedlings</subject><subject>seeds</subject><subject>somatic embryogenesis</subject><subject>Spruce</subject><subject>Thawing</subject><subject>tissue culture</subject><subject>tree breeding</subject><subject>Trees</subject><subject>Vegetation</subject><subject>yields</subject><issn>0045-5067</issn><issn>1208-6037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp90V1rFDEUBuAgCq5V_AkGkQqV0XxMJhPvSq1aKChovY1J5mSaspOsyQx1_70psygIepME8uQ95ByEnlLymlKu3vxUfUO5vIc2lJG-6QiX99GGkFY0gnTyIXpUyg0hhHecbND3izjDmM0cUsTJ45KmenYYJpv3aYQIJRQcIjZ4zgDY1mUIccS7nOqzaYK39cqZArjMy7DHt2G-xp-DA4ONDVAeowfebAs8OexH6Or9-dezj83lpw8XZ6eXjeOCysaRTnnGWtl5sIMEYa0gtGU9ZaA4kIEPUrUWWOel4kYJb4iH1t15LozlR-jVmltuYbdYvcthMnmvkwn6Xfh2qlMe9bJo1jMqqz5edf3GjwXKrKdQHGy3JkJaima1g6JnvMLnf8GbtORYf6IZJ4KpXrUVvVyRy6mUDP53dUr03VB0HYqukVW-OMSZ4szWZxNdKH94J0TLWWUnK4vZZShgsrv-T-bxv_EB6d3gK3y2Qm-SNmOuha--MEJ5bbTsOen4L7eDsD8</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Hogberg, K.A</creator><creator>Ekberg, I</creator><creator>Norell, L</creator><creator>Arnold, S. von</creator><general>NRC Research Press</general><general>National Research Council of Canada</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8AF</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>U9A</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DF2</scope></search><sort><creationdate>19981001</creationdate><title>Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies</title><author>Hogberg, K.A ; Ekberg, I ; Norell, L ; Arnold, S. von</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3517-c069f22476febd7e5bb50142812e93e0d3d794be26f793a95fa0fe4c76fe35ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>agricultural programs and projects</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Breeding</topic><topic>Calculations</topic><topic>Case studies</topic><topic>cryopreservation</topic><topic>Cuttings</topic><topic>embryo (plant)</topic><topic>Embryology</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Eukaryotic cell cultures</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genotype</topic><topic>Genotypes</topic><topic>height</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>maturation</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>phenology</topic><topic>Picea abies</topic><topic>Pine trees</topic><topic>Plant breeding</topic><topic>Plant cells and fungal cells</topic><topic>plant characteristics</topic><topic>Plant production</topic><topic>Plant propagation</topic><topic>Plants (botany)</topic><topic>regenerative ability</topic><topic>seedlings</topic><topic>seeds</topic><topic>somatic embryogenesis</topic><topic>Spruce</topic><topic>Thawing</topic><topic>tissue culture</topic><topic>tree breeding</topic><topic>Trees</topic><topic>Vegetation</topic><topic>yields</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hogberg, K.A</creatorcontrib><creatorcontrib>Ekberg, I</creatorcontrib><creatorcontrib>Norell, L</creatorcontrib><creatorcontrib>Arnold, S. von</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Uppsala universitet</collection><jtitle>Canadian journal of forest research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hogberg, K.A</au><au>Ekberg, I</au><au>Norell, L</au><au>Arnold, S. von</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies</atitle><jtitle>Canadian journal of forest research</jtitle><addtitle>Revue canadienne de recherche forestière</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>28</volume><issue>10</issue><spage>1536</spage><epage>1545</epage><pages>1536-1545</pages><issn>0045-5067</issn><eissn>1208-6037</eissn><coden>CJFRAR</coden><abstract>Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessity when dealing with a large number of genotypes. Proliferation and maturation were identified as major constraints. Clear differences among families were seen for proliferation and for maturation. For plant regeneration, differences among cell lines within families were considerable while no significant differences were found among families. Significant differences among male parents were obtained for proliferation and maturation. Our calculations show that by using an improved standard protocol, at least one third of the genotypes can be propagated via somatic embryogenesis. No clear relationship was found between embryogenic characters of selected parents based on progenies and corresponding parental breeding values for growth and phenology characters. Three years can be gained by using somatic embryogenesis instead of cuttings in a breeding programme. Maintained propagation ability by including cryopreservation in the breeding programme will provide a very useful link between breeding and mass propagation. The results are encouraging, but further improvement of the standard protocol is necessary.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><doi>10.1139/x98-137</doi><tpages>10</tpages></addata></record> |
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| ispartof | Canadian journal of forest research, 1998-10, Vol.28 (10), p.1536-1545 |
| issn | 0045-5067 1208-6037 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | agricultural programs and projects Biological and medical sciences Biotechnology Breeding Calculations Case studies cryopreservation Cuttings embryo (plant) Embryology Embryonic growth stage Embryos Eukaryotic cell cultures Forestry Fundamental and applied biological sciences. Psychology genotype Genotypes height In vitro propagation: entire plant regeneration from tissues and cell cultures maturation Methods. Procedures. Technologies micropropagation phenology Picea abies Pine trees Plant breeding Plant cells and fungal cells plant characteristics Plant production Plant propagation Plants (botany) regenerative ability seedlings seeds somatic embryogenesis Spruce Thawing tissue culture tree breeding Trees Vegetation yields |
| title | Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies |
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