Platelet deposition on stainless steel, spiral, and braided polylactide stents A comparative study
Platelets play a key role in (sub)acute thrombotic occlusion after stenting. We examined the possible differences between biodegradable polylactide (PLA) and stainless steel (SS) stents in platelet attachment and morphology after whole blood perfusion. PLA stents of different configurations (spiral/...
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| Veröffentlicht in: | Thrombosis and haemostasis 2004-12, Vol.92 (6), p.1394-1401 |
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| creator | HIETALA, Eeva-Maija MAASILTA, Paula JUUTI, Hanne NUUTINEN, Juha-Pekka HARJULA, Ari L. J SALMINEN, Ulla-Stina LASSILA, Riitta |
| description | Platelets play a key role in (sub)acute thrombotic occlusion after stenting. We examined the possible differences between biodegradable polylactide (PLA) and stainless steel (SS) stents in platelet attachment and morphology after whole blood perfusion. PLA stents of different configurations (spiral/braided) and polycaprolactone-polylactide (PCL-PLA)-coatings, or SS stents were implanted into a PVC tube (Ø 3.2 mm), with or without precoating of the tube with type-I collagen. PPACK (30 microM)-anticoagulated blood with (3)H-serotonin prelabeled platelets was perfused (flow rate: 30 ml/min, 90 s) over the stents. Platelet deposition was assessed by scintillation counting and morphology by scanning electron microscopy (SEM). To examine coagulation activation, plasma prothrombin fragments (F1 + 2) were measured before and after the perfusion. Protein deposition on PLA/SS stents was assessed at augmented shear forces mimicking coronary flow (rate: 60 ml/min, 60 s) under minimal anticoagulation (PPACK 1 microM). More platelets deposited on PLA stents than on SS stents under all study conditions (p < 0.03). Under anticoagulation (PPACK 30 microM) the generation of F1 + 2 remained unaltered. Under higher flow rate and limited anticoagulation SS stents accumulated 3.27 +/- 0.75 microg and PLA stents 5.25 +/- 1.74 microg of protein (Mean +/- SD, p |
| doi_str_mv | 10.1160/TH04-02-0124 |
| format | Article |
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J ; SALMINEN, Ulla-Stina ; LASSILA, Riitta</creator><creatorcontrib>HIETALA, Eeva-Maija ; MAASILTA, Paula ; JUUTI, Hanne ; NUUTINEN, Juha-Pekka ; HARJULA, Ari L. J ; SALMINEN, Ulla-Stina ; LASSILA, Riitta</creatorcontrib><description>Platelets play a key role in (sub)acute thrombotic occlusion after stenting. We examined the possible differences between biodegradable polylactide (PLA) and stainless steel (SS) stents in platelet attachment and morphology after whole blood perfusion. PLA stents of different configurations (spiral/braided) and polycaprolactone-polylactide (PCL-PLA)-coatings, or SS stents were implanted into a PVC tube (Ø 3.2 mm), with or without precoating of the tube with type-I collagen. PPACK (30 microM)-anticoagulated blood with (3)H-serotonin prelabeled platelets was perfused (flow rate: 30 ml/min, 90 s) over the stents. Platelet deposition was assessed by scintillation counting and morphology by scanning electron microscopy (SEM). To examine coagulation activation, plasma prothrombin fragments (F1 + 2) were measured before and after the perfusion. Protein deposition on PLA/SS stents was assessed at augmented shear forces mimicking coronary flow (rate: 60 ml/min, 60 s) under minimal anticoagulation (PPACK 1 microM). More platelets deposited on PLA stents than on SS stents under all study conditions (p < 0.03). Under anticoagulation (PPACK 30 microM) the generation of F1 + 2 remained unaltered. Under higher flow rate and limited anticoagulation SS stents accumulated 3.27 +/- 0.75 microg and PLA stents 5.25 +/- 1.74 microg of protein (Mean +/- SD, p <0.95). Among all biodegradable stents, the braided PLA stent coated with PCL-PLA-heparin accumulated the fewest platelets (p < 0.02). In SEM, signs of platelet activation on braided heparin-coated PLA stents, when compared with uncoated braided PLA/SS stents, appeared modest. In conclusion, PCL-PLAheparin coating of biodegradable stents may enhance their hemocompatibility, expressed by less platelet deposition. Nevertheless, materials, design, and coating techniques of biodegradable stents must be further developed.</description><identifier>ISSN: 0340-6245</identifier><identifier>EISSN: 2567-689X</identifier><identifier>DOI: 10.1160/TH04-02-0124</identifier><identifier>PMID: 15583749</identifier><identifier>CODEN: THHADQ</identifier><language>eng</language><publisher>Stuttgart: Schattauer Verlag für Medizin und Naturwissenschaften</publisher><subject>Amino Acid Chloromethyl Ketones - chemistry ; Biocompatible Materials - chemistry ; Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - cytology ; Blood Platelets - physiology ; Blood Platelets - ultrastructure ; Cell Culture Techniques - instrumentation ; Collagen - metabolism ; Collagen Type I - chemistry ; Coronary Vessels - metabolism ; Fundamental and applied biological sciences. Psychology ; Hematologic and hematopoietic diseases ; Heparin - chemistry ; Humans ; Medical sciences ; Microscopy, Electron, Scanning ; Molecular and cellular biology ; Perfusion ; Platelet Adhesiveness ; Platelet diseases and coagulopathies ; Polyesters - chemistry ; Polyvinyl Chloride - chemistry ; Prothrombin - biosynthesis ; Serotonin - metabolism ; Stainless Steel - chemistry ; Stents ; Time Factors</subject><ispartof>Thrombosis and haemostasis, 2004-12, Vol.92 (6), p.1394-1401</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16331018$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15583749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HIETALA, Eeva-Maija</creatorcontrib><creatorcontrib>MAASILTA, Paula</creatorcontrib><creatorcontrib>JUUTI, Hanne</creatorcontrib><creatorcontrib>NUUTINEN, Juha-Pekka</creatorcontrib><creatorcontrib>HARJULA, Ari L. J</creatorcontrib><creatorcontrib>SALMINEN, Ulla-Stina</creatorcontrib><creatorcontrib>LASSILA, Riitta</creatorcontrib><title>Platelet deposition on stainless steel, spiral, and braided polylactide stents A comparative study</title><title>Thrombosis and haemostasis</title><addtitle>Thromb Haemost</addtitle><description>Platelets play a key role in (sub)acute thrombotic occlusion after stenting. We examined the possible differences between biodegradable polylactide (PLA) and stainless steel (SS) stents in platelet attachment and morphology after whole blood perfusion. PLA stents of different configurations (spiral/braided) and polycaprolactone-polylactide (PCL-PLA)-coatings, or SS stents were implanted into a PVC tube (Ø 3.2 mm), with or without precoating of the tube with type-I collagen. PPACK (30 microM)-anticoagulated blood with (3)H-serotonin prelabeled platelets was perfused (flow rate: 30 ml/min, 90 s) over the stents. Platelet deposition was assessed by scintillation counting and morphology by scanning electron microscopy (SEM). To examine coagulation activation, plasma prothrombin fragments (F1 + 2) were measured before and after the perfusion. Protein deposition on PLA/SS stents was assessed at augmented shear forces mimicking coronary flow (rate: 60 ml/min, 60 s) under minimal anticoagulation (PPACK 1 microM). More platelets deposited on PLA stents than on SS stents under all study conditions (p < 0.03). Under anticoagulation (PPACK 30 microM) the generation of F1 + 2 remained unaltered. Under higher flow rate and limited anticoagulation SS stents accumulated 3.27 +/- 0.75 microg and PLA stents 5.25 +/- 1.74 microg of protein (Mean +/- SD, p <0.95). Among all biodegradable stents, the braided PLA stent coated with PCL-PLA-heparin accumulated the fewest platelets (p < 0.02). In SEM, signs of platelet activation on braided heparin-coated PLA stents, when compared with uncoated braided PLA/SS stents, appeared modest. In conclusion, PCL-PLAheparin coating of biodegradable stents may enhance their hemocompatibility, expressed by less platelet deposition. Nevertheless, materials, design, and coating techniques of biodegradable stents must be further developed.</description><subject>Amino Acid Chloromethyl Ketones - chemistry</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - cytology</subject><subject>Blood Platelets - physiology</subject><subject>Blood Platelets - ultrastructure</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Collagen - metabolism</subject><subject>Collagen Type I - chemistry</subject><subject>Coronary Vessels - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Heparin - chemistry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molecular and cellular biology</subject><subject>Perfusion</subject><subject>Platelet Adhesiveness</subject><subject>Platelet diseases and coagulopathies</subject><subject>Polyesters - chemistry</subject><subject>Polyvinyl Chloride - chemistry</subject><subject>Prothrombin - biosynthesis</subject><subject>Serotonin - metabolism</subject><subject>Stainless Steel - chemistry</subject><subject>Stents</subject><subject>Time Factors</subject><issn>0340-6245</issn><issn>2567-689X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqtkc-L1TAQx4so7nP15ll60dNWk-Znj8uirrCghxW8hWma0kja1EyqvP_e1PdwwbMQmMzwYb7wmap6SclbSiV5d39LeEPahtCWP6oOrZCqkbr79rg6EMZJI1suLqpniN8JoZJ34ml1QYXQTPHuUPVfAmQXXK4Ht0b02celLg8z-CU4xPJzLlzVuPoEpcIy1H0CP7ihXmM4BrC5NDu2ZKyvaxvnFRJk_3MfbsPxefVkhIDuxbleVl8_vL-_uW3uPn_8dHN911guZW44AGsZ6TR0Qmo1cjWOXdcpplvlWksEc6MarO1HgJYoEEqrQeoeup5ppym7rN6c9q4p_tgcZjN7tC4EWFzc0EhFBeOyLeDVCbQpIiY3mjX5GdLRUGJ2p2Z3akhrdqcFf3Xeu_WzGx7gs8QCvD4DgBbCmGCxHh84yRglVBfOnzi0E-QMm0t_oTylOPflAGiKYTOBm2O5wd7buPxxayDZqVg1HnFzRmlhZlg2tMmv2bCOC4NT_GWmPIeSZf9jFq7Oegj_5rHfMSXQ0Q</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>HIETALA, Eeva-Maija</creator><creator>MAASILTA, Paula</creator><creator>JUUTI, Hanne</creator><creator>NUUTINEN, Juha-Pekka</creator><creator>HARJULA, Ari L. J</creator><creator>SALMINEN, Ulla-Stina</creator><creator>LASSILA, Riitta</creator><general>Schattauer Verlag für Medizin und Naturwissenschaften</general><general>Schattauer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Platelet deposition on stainless steel, spiral, and braided polylactide stents A comparative study</title><author>HIETALA, Eeva-Maija ; MAASILTA, Paula ; JUUTI, Hanne ; NUUTINEN, Juha-Pekka ; HARJULA, Ari L. J ; SALMINEN, Ulla-Stina ; LASSILA, Riitta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-4aa323098a95687f47ff99973827e2c053ef7dccbfaa207a5787d68ba9b38e813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Chloromethyl Ketones - chemistry</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - cytology</topic><topic>Blood Platelets - physiology</topic><topic>Blood Platelets - ultrastructure</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Collagen - metabolism</topic><topic>Collagen Type I - chemistry</topic><topic>Coronary Vessels - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Heparin - chemistry</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molecular and cellular biology</topic><topic>Perfusion</topic><topic>Platelet Adhesiveness</topic><topic>Platelet diseases and coagulopathies</topic><topic>Polyesters - chemistry</topic><topic>Polyvinyl Chloride - chemistry</topic><topic>Prothrombin - biosynthesis</topic><topic>Serotonin - metabolism</topic><topic>Stainless Steel - chemistry</topic><topic>Stents</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HIETALA, Eeva-Maija</creatorcontrib><creatorcontrib>MAASILTA, Paula</creatorcontrib><creatorcontrib>JUUTI, Hanne</creatorcontrib><creatorcontrib>NUUTINEN, Juha-Pekka</creatorcontrib><creatorcontrib>HARJULA, Ari L. J</creatorcontrib><creatorcontrib>SALMINEN, Ulla-Stina</creatorcontrib><creatorcontrib>LASSILA, Riitta</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis and haemostasis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIETALA, Eeva-Maija</au><au>MAASILTA, Paula</au><au>JUUTI, Hanne</au><au>NUUTINEN, Juha-Pekka</au><au>HARJULA, Ari L. J</au><au>SALMINEN, Ulla-Stina</au><au>LASSILA, Riitta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet deposition on stainless steel, spiral, and braided polylactide stents A comparative study</atitle><jtitle>Thrombosis and haemostasis</jtitle><addtitle>Thromb Haemost</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>92</volume><issue>6</issue><spage>1394</spage><epage>1401</epage><pages>1394-1401</pages><issn>0340-6245</issn><eissn>2567-689X</eissn><coden>THHADQ</coden><abstract>Platelets play a key role in (sub)acute thrombotic occlusion after stenting. We examined the possible differences between biodegradable polylactide (PLA) and stainless steel (SS) stents in platelet attachment and morphology after whole blood perfusion. PLA stents of different configurations (spiral/braided) and polycaprolactone-polylactide (PCL-PLA)-coatings, or SS stents were implanted into a PVC tube (Ø 3.2 mm), with or without precoating of the tube with type-I collagen. PPACK (30 microM)-anticoagulated blood with (3)H-serotonin prelabeled platelets was perfused (flow rate: 30 ml/min, 90 s) over the stents. Platelet deposition was assessed by scintillation counting and morphology by scanning electron microscopy (SEM). To examine coagulation activation, plasma prothrombin fragments (F1 + 2) were measured before and after the perfusion. Protein deposition on PLA/SS stents was assessed at augmented shear forces mimicking coronary flow (rate: 60 ml/min, 60 s) under minimal anticoagulation (PPACK 1 microM). More platelets deposited on PLA stents than on SS stents under all study conditions (p < 0.03). Under anticoagulation (PPACK 30 microM) the generation of F1 + 2 remained unaltered. Under higher flow rate and limited anticoagulation SS stents accumulated 3.27 +/- 0.75 microg and PLA stents 5.25 +/- 1.74 microg of protein (Mean +/- SD, p <0.95). Among all biodegradable stents, the braided PLA stent coated with PCL-PLA-heparin accumulated the fewest platelets (p < 0.02). In SEM, signs of platelet activation on braided heparin-coated PLA stents, when compared with uncoated braided PLA/SS stents, appeared modest. In conclusion, PCL-PLAheparin coating of biodegradable stents may enhance their hemocompatibility, expressed by less platelet deposition. Nevertheless, materials, design, and coating techniques of biodegradable stents must be further developed.</abstract><cop>Stuttgart</cop><pub>Schattauer Verlag für Medizin und Naturwissenschaften</pub><pmid>15583749</pmid><doi>10.1160/TH04-02-0124</doi><tpages>8</tpages></addata></record> |
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| ispartof | Thrombosis and haemostasis, 2004-12, Vol.92 (6), p.1394-1401 |
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| subjects | Amino Acid Chloromethyl Ketones - chemistry Biocompatible Materials - chemistry Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - cytology Blood Platelets - physiology Blood Platelets - ultrastructure Cell Culture Techniques - instrumentation Collagen - metabolism Collagen Type I - chemistry Coronary Vessels - metabolism Fundamental and applied biological sciences. Psychology Hematologic and hematopoietic diseases Heparin - chemistry Humans Medical sciences Microscopy, Electron, Scanning Molecular and cellular biology Perfusion Platelet Adhesiveness Platelet diseases and coagulopathies Polyesters - chemistry Polyvinyl Chloride - chemistry Prothrombin - biosynthesis Serotonin - metabolism Stainless Steel - chemistry Stents Time Factors |
| title | Platelet deposition on stainless steel, spiral, and braided polylactide stents A comparative study |
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