Biotransformation of the Fungicide Chlorothalonil by Bacterial Glutathione S-Transferase

A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, an isolated bacterium strain from soil. We determined the nucleotide sequences and found an open reading frame for glutathione S-transferase (GST). The drug-hypersensitive...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2004-10, Vol.14 (5), p.938-943
Hauptverfasser:	Kim, Y.M. (Kyungpook National University, Daegu, Republic of Korea), E-mail: ikrhee@knu.ac.kr, Park, K.B. (Kyungpook National University, Daegu, Republic of Korea), Choi, J.H. (Kyungpook National University, Daegu, Republic of Korea), Kim, J.E. (Kyungpook National University, Daegu, Republic of Korea), Rhee, I.K. (Kyungpook National University, Daegu, Republic of Korea)
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology CHLOROTHALONIL CLOROTALONIL Fundamental and applied biological sciences. Psychology GLUTATHION GLUTATHIONE GLUTATION Ochrobactrum anthropi SH35B TRANSFERASAS TRANSFERASE TRANSFERASES
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Zusammenfassung:	A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, an isolated bacterium strain from soil. We determined the nucleotide sequences and found an open reading frame for glutathione S-transferase (GST). The drug-hypersensitive Escherichia coli KAM3 cells transformed with a plasmid carrying the GST gene can grow in the presence of chlorothalonil. The GST of O. anthropi SH35B was expressed in E. coli and purified by affinity chromatography.
ISSN:	1017-7825