Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase
Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carba...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2004-08, Vol.14 (4), p.845-851 |
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| creator | Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea) Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea) Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea) Kim, H.J. (Chung Ang University, Seoul, Republic of Korea) Choi, J.H. (Chung Ang University, Seoul, Republic of Korea) Kim, J.K. (Chung Ang University, Seoul, Republic of Korea) Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr |
| description | Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE. |
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(Chung Ang University, Seoul, Republic of Korea) ; Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea) ; Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) ; Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea) ; Choi, J.H. (Chung Ang University, Seoul, Republic of Korea) ; Kim, J.K. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr</creator><creatorcontrib>Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea) ; Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea) ; Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) ; Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea) ; Choi, J.H. (Chung Ang University, Seoul, Republic of Korea) ; Kim, J.K. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr</creatorcontrib><description>Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>AMMONIA ; ammonia concentration ; AMMONIAC ; AMONIACO ; Biological and medical sciences ; Biotechnology ; ERITROPOIETINA ; ERYTHROPOIETIN ; ERYTHROPOIETINE ; Fundamental and applied biological sciences. Psychology ; sialylation ; urea cycle enzymes</subject><ispartof>Journal of microbiology and biotechnology, 2004-08, Vol.14 (4), p.845-851</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16064687$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea)</creatorcontrib><creatorcontrib>Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, H.J. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Choi, J.H. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.K. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr</creatorcontrib><title>Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase</title><title>Journal of microbiology and biotechnology</title><description>Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE.</description><subject>AMMONIA</subject><subject>ammonia concentration</subject><subject>AMMONIAC</subject><subject>AMONIACO</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ERITROPOIETINA</subject><subject>ERYTHROPOIETIN</subject><subject>ERYTHROPOIETINE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>sialylation</subject><subject>urea cycle enzymes</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpFj09Lw0AQxXNQsFY_gjAXQcHC7DZJN0cJ1RYLLVrPZZJMTCTZDburEL-Z3871D8gc3uX33pt3FE0EisVsoWRyEp0694qYCqnSSfS51A3pknvWHkwNSzv6xprBtOxbDTtrqrfSt0ZDbU0PedNqdgwr6p1nC9t3suNVvtpeQ85d56AYYa39vytE-obh2TJBPpYdw1J_jD27G8jJFtSbsYNdY9zQkGd4GnXAPYWKNZCuYGt1679LYW9Ju_LHM3YBOIuOa-ocn__pNNrfLff5arbZ3q_z282sTmSYnBBmSjEqLJApkxhjoTAWJQrmOC6lKmQhlcwwqyqZzMsAKRFuXmNF9XwaXf7GDhTauzp8UbbuMNi2D9MPIsU0TtUicBe_XE3mQC82MA-PEjFBIQXi_AsdoXls</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea)</creator><creator>Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea)</creator><creator>Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea)</creator><creator>Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea)</creator><creator>Kim, H.J. (Chung Ang University, Seoul, Republic of Korea)</creator><creator>Choi, J.H. (Chung Ang University, Seoul, Republic of Korea)</creator><creator>Kim, J.K. (Chung Ang University, Seoul, Republic of Korea)</creator><creator>Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>200408</creationdate><title>Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase</title><author>Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea) ; Chang, K.H. (Samsung Fine Chemicals Co., LTD. RnD Center, Seoul, Republic of Korea) ; Kim, J.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) ; Lee, Y.J. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea) ; Choi, J.H. (Chung Ang University, Seoul, Republic of Korea) ; Kim, J.K. (Chung Ang University, Seoul, Republic of Korea) ; Kim, H.J. (Chung Ang University, Seoul, Republic of Korea), E-mail: hongjink@cau.ac.kr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f527-75a0988e080b0ea92040b8041c01ee44c28b2b282909dd253cea9818183f0daf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>AMMONIA</topic><topic>ammonia concentration</topic><topic>AMMONIAC</topic><topic>AMONIACO</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>ERITROPOIETINA</topic><topic>ERYTHROPOIETIN</topic><topic>ERYTHROPOIETINE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>sialylation</topic><topic>urea cycle enzymes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, N.Y. (Chung Ang University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Chang, K.H. (Samsung Fine Chemicals Co., LTD. 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To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><tpages>7</tpages></addata></record> |
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| ispartof | Journal of microbiology and biotechnology, 2004-08, Vol.14 (4), p.845-851 |
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| subjects | AMMONIA ammonia concentration AMMONIAC AMONIACO Biological and medical sciences Biotechnology ERITROPOIETINA ERYTHROPOIETIN ERYTHROPOIETINE Fundamental and applied biological sciences. Psychology sialylation urea cycle enzymes |
| title | Enhancement of Erythropoietin Production from Chinese Hamster Ovary(CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase I and Ornithine Transcarbamylase |
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